LOCUS BBa_K1795000 4113 bp DNA linear 22-Nov-2024 DEFINITION E. Coli Codon Optimized dCAS9, solo ACCESSION BBa_K1795000 VERSION BBa_K1795000.1 FEATURES Location/Qualifiers ORIGIN 1 atggacaaaa aatacagtat cggcctggcg atcggcacca attcagttgg ctgggcagtt 61 attactgatg aatacaaggt cccctccaaa aagtttaaag tcctgggcaa caccgatcgt 121 cacagcatca aaaaaaatct gatcggagcc ttgttattcg acagtggtga gacggcagaa 181 gccacccgtt tgaagcgtac tgcccgccgt cgctacacac gtcgtaagaa ccgtatctgt 241 tacttgcagg aaattttctc aaatgaaatg gcgaaagtgg acgacagctt cttccaccgc 301 cttgaagagt cattcttagt tgaggaagac aaaaaacacg aacgtcatcc tatctttggc 361 aatatcgtcg atgaagtcgc gtatcacgaa aagtacccga ccatttatca cttgcgtaaa 421 aaactggtcg actcgaccga caaagcagac ctgcgtctga tttatttagc attggcccat 481 atgattaaat ttcgcggcca cttcttaatc gaaggcgact tgaatccgga caactcggat 541 gtggataaat tgttcattca gctggtccag acgtataatc agctttttga ggaaaacccg 601 atcaacgcat cgggcgttga tgccaaggcg attttatccg cacgcctgtc gaaaagccgc 661 cgtctggaaa atctcatcgc acaactgccg ggcgaaaaga aaaacggtct gttcgggaac 721 ttgattgcac tgtccctggg cctgacccca aactttaaaa gcaatttcga cttggccgaa 781 gatgcgaagc tgcagctgag taaagacacc tatgatgacg acttagataa tttgttggcg 841 cagattggtg atcaatacgc cgacctcttt ctcgcagcga aaaatctgag tgatgcgatc 901 ttattatctg atatcctgcg cgtcaacacc gaaattacca aagcaccgct gtccgccagt 961 atgatcaagc gctacgacga gcatcatcaa gatctgaccc ttctcaaagc gttagtgcgc 1021 cagcagctgc cggaaaaata caaagagatc ttcttcgacc agagtaaaaa cggatatgca 1081 ggttacattg atggcggcgc atcacaggaa gaattttata aatttattaa accaatttta 1141 gagaaaatgg atggtactga agaactcctt gtcaaactga accgcgaaga tcttctgcgc 1201 aaacaacgca cgtttgacaa cggttccatt ccgcatcaaa ttcatttggg agaactccat 1261 gctattctgc gtcgtcagga agatttctat ccgtttttga aggataatcg tgagaagatt 1321 gagaaaatcc tgacatttcg tatcccttac tatgtcggtc ctctggcgcg cggcaacagc 1381 cgctttgcat ggatgactcg taaaagcgag gaaacgatta ccccgtggaa tttcgaagaa 1441 gtagtggaca aaggagcgtc ggcgcagagt tttattgaac gcatgaccaa tttcgataaa 1501 aatctgccga acgaaaaagt actgccaaaa cactctctgc tgtatgaata cttcaccgtg 1561 tacaatgaac tcactaaggt caaatatgta acagaaggta tgcgtaaacc cgcgttttta 1621 agcggggaac aaaaaaaggc catcgtagac cttctcttca aaacgaaccg caaggtaacc 1681 gtgaaacagt taaaagagga ctatttcaaa aaaatcgaat gttttgacag cgtggagatc 1741 tcaggtgtgg aagatcgctt caacgcttca ctcggaactt accatgatct gttaaagatc 1801 attaaagaca aggattttct tgataacgaa gagaacgaag acattttaga ggatatcgtc 1861 ctgacactga ctttgtttga agatcgcgaa atgattgaag aacgcctgaa aacctatgcg 1921 catctgtttg atgacaaagt catgaaacag ctgaaacgtc gtcgctatac tggctggggc 1981 cgtctgagtc gcaaactcat taacggtatt cgtgacaaac aaagtggtaa aacgattctg 2041 gattttctta aaagcgacgg atttgcgaat cgtaacttca tgcaattaat ccacgacgat 2101 tcgctgacat ttaaagagga cattcaaaaa gctcaggtga gtggccaggg tgacagcttg 2161 catgagcata ttgcgaactt agctggtagc cctgccatta aaaaaggcat tttgcagact 2221 gtaaaagtgg tggacgaact ggttaaggtg atgggccgcc acaaaccaga aaatatcgtt 2281 attgaaatgg cacgcgaaaa ccagactacg caaaaaggtc agaaaaactc tcgtgaacgt 2341 atgaaacgca tcgaagaagg aatcaaagaa ctgggtagcc agatcctgaa agaacacccg 2401 gtggaaaaca cgcaactgca gaacgaaaaa ctgtatctct attatttaca aaatggtcgc 2461 gacatgtatg tggatcaaga attggatatt aatcgcttgt ccgattatga cgtcgatgcg 2521 attgttccgc agtccttcct gaaagatgac agcattgata acaaagtttt aactcgctcg 2581 gacaaaaacc gcgggaaatc tgataacgtc ccgagcgagg aggtagtgaa aaagatgaaa 2641 aactattggc gccagctgct gaacgcgaaa ctcatcaccc agcgtaaatt tgataacctt 2701 acaaaagcag aacgtggtgg tttgtccgag ctggataagg cgggcttcat caaacgccag 2761 ctcgtagaga cgcgccaaat cactaaacat gtggcgcaaa tcctggatag ccgtatgaat 2821 acgaagtacg atgagaatga caaactgatt cgcgaagtca aagtgatcac cctgaaatct 2881 aagttggtga gcgattttcg caaagacttt cagttttata aggttcgcga gattaacaac 2941 tatcatcatg cgcacgatgc ctacttaaat gccgtggtgg gcaccgctct catcaaaaaa 3001 tacccaaaat tggagtcaga gtttgtttac ggtgattata aagtttatga tgttcgtaaa 3061 atgatcgcaa aaagcgagca ggagatcgga aaagcaacag ccaagtattt tttttatagc 3121 aatattatga attttttcaa aacagaaatc accttagcga atggcgaaat ccgtaagcgc 3181 cctcttattg aaacaaatgg ggaaaccggc gaaattgtct gggataaggg ccgcgatttt 3241 gccactgtgc gtaaagtgct gtctatgcca caggttaata tcgtgaaaaa aaccgaggta 3301 cagaccggtg ggtttagtaa agaaagcatt ctgcccaagc gcaacagcga taaactgatt 3361 gcgcgcaaaa aagattggga tccgaaaaaa tatggtggct tcgattcgcc tactgttgcg 3421 tattcggtcc tcgtagtggc taaggtagaa aaaggtaaat ctaaaaaatt aaaaagcgta 3481 aaagaattac tgggaattac gattatggaa cgtagctcgt ttgagaaaaa cccgattgac 3541 ttcctggaag cgaaaggata taaagaagtt aaaaaagatc tgatcattaa gctgccgaaa 3601 tactcgctct ttgaactcga aaacggtcgt aaacgcatgc tggcatcagc aggcgagctg 3661 cagaaaggca acgagcttgc gttgccctcc aaatacgtca atttcttgta tctggctagt 3721 cattacgaaa aattgaaagg atccccggaa gacaacgaac aaaaacaact gttcgtggaa 3781 cagcacaaac attatcttga cgagatcatt gaacagatct ctgaattttc caaacgtgtt 3841 attctggccg atgccaacct ggacaaggtt ctttctgcgt acaacaaaca tcgcgacaaa 3901 ccaatccgcg agcaagctga aaatattatt catttattta cactgacaaa cctgggtgcg 3961 ccggcggcgt ttaaatattt cgatacaacc atcgatcgca agcgttacac ctctaccaaa 4021 gaagtgctgg atgccaccct gattcatcaa agcattacgg gtctgtacga aacgcgcatt 4081 gatctgagtc agctgggcgg ggactaagtc gac //