BBa_K1795000 1 BBa_K1795000 E. Coli Codon Optimized dCAS9, solo 2015-09-16T11:00:00Z 2015-09-17T09:39:49Z The basic template for the sgRNA sequence was taken from the supplementary information of Qi LS, Larson MH, Gilbert LA, et al. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013;152(5):1173-1183. doi:10.1016/j.cell.2013.02.022. This sgRNA sequence was designed to target the Promoter Bba_R0010 with the N20 sequence atgttgtgtggaattgtgag. The part is under the control of the promoter Bba_J23100. If this gRNA is expressed in a cell with a form of the dCas9 protein, the protein will be targeted to the N20 sequence and repress gene expression. Expression of this part is repressed in the presence of LacI and CAP protein. false false _2220_ 27446 21426 9 false Ensure the promoter targeted by this part is not used within this part. false Andrew D Halleran, Joseph L Maniaci BBa_K1795000_sequence 1 atggacaaaaaatacagtatcggcctggcgatcggcaccaattcagttggctgggcagttattactgatgaatacaaggtcccctccaaaaagtttaaagtcctgggcaacaccgatcgtcacagcatcaaaaaaaatctgatcggagccttgttattcgacagtggtgagacggcagaagccacccgtttgaagcgtactgcccgccgtcgctacacacgtcgtaagaaccgtatctgttacttgcaggaaattttctcaaatgaaatggcgaaagtggacgacagcttcttccaccgccttgaagagtcattcttagttgaggaagacaaaaaacacgaacgtcatcctatctttggcaatatcgtcgatgaagtcgcgtatcacgaaaagtacccgaccatttatcacttgcgtaaaaaactggtcgactcgaccgacaaagcagacctgcgtctgatttatttagcattggcccatatgattaaatttcgcggccacttcttaatcgaaggcgacttgaatccggacaactcggatgtggataaattgttcattcagctggtccagacgtataatcagctttttgaggaaaacccgatcaacgcatcgggcgttgatgccaaggcgattttatccgcacgcctgtcgaaaagccgccgtctggaaaatctcatcgcacaactgccgggcgaaaagaaaaacggtctgttcgggaacttgattgcactgtccctgggcctgaccccaaactttaaaagcaatttcgacttggccgaagatgcgaagctgcagctgagtaaagacacctatgatgacgacttagataatttgttggcgcagattggtgatcaatacgccgacctctttctcgcagcgaaaaatctgagtgatgcgatcttattatctgatatcctgcgcgtcaacaccgaaattaccaaagcaccgctgtccgccagtatgatcaagcgctacgacgagcatcatcaagatctgacccttctcaaagcgttagtgcgccagcagctgccggaaaaatacaaagagatcttcttcgaccagagtaaaaacggatatgcaggttacattgatggcggcgcatcacaggaagaattttataaatttattaaaccaattttagagaaaatggatggtactgaagaactccttgtcaaactgaaccgcgaagatcttctgcgcaaacaacgcacgtttgacaacggttccattccgcatcaaattcatttgggagaactccatgctattctgcgtcgtcaggaagatttctatccgtttttgaaggataatcgtgagaagattgagaaaatcctgacatttcgtatcccttactatgtcggtcctctggcgcgcggcaacagccgctttgcatggatgactcgtaaaagcgaggaaacgattaccccgtggaatttcgaagaagtagtggacaaaggagcgtcggcgcagagttttattgaacgcatgaccaatttcgataaaaatctgccgaacgaaaaagtactgccaaaacactctctgctgtatgaatacttcaccgtgtacaatgaactcactaaggtcaaatatgtaacagaaggtatgcgtaaacccgcgtttttaagcggggaacaaaaaaaggccatcgtagaccttctcttcaaaacgaaccgcaaggtaaccgtgaaacagttaaaagaggactatttcaaaaaaatcgaatgttttgacagcgtggagatctcaggtgtggaagatcgcttcaacgcttcactcggaacttaccatgatctgttaaagatcattaaagacaaggattttcttgataacgaagagaacgaagacattttagaggatatcgtcctgacactgactttgtttgaagatcgcgaaatgattgaagaacgcctgaaaacctatgcgcatctgtttgatgacaaagtcatgaaacagctgaaacgtcgtcgctatactggctggggccgtctgagtcgcaaactcattaacggtattcgtgacaaacaaagtggtaaaacgattctggattttcttaaaagcgacggatttgcgaatcgtaacttcatgcaattaatccacgacgattcgctgacatttaaagaggacattcaaaaagctcaggtgagtggccagggtgacagcttgcatgagcatattgcgaacttagctggtagccctgccattaaaaaaggcattttgcagactgtaaaagtggtggacgaactggttaaggtgatgggccgccacaaaccagaaaatatcgttattgaaatggcacgcgaaaaccagactacgcaaaaaggtcagaaaaactctcgtgaacgtatgaaacgcatcgaagaaggaatcaaagaactgggtagccagatcctgaaagaacacccggtggaaaacacgcaactgcagaacgaaaaactgtatctctattatttacaaaatggtcgcgacatgtatgtggatcaagaattggatattaatcgcttgtccgattatgacgtcgatgcgattgttccgcagtccttcctgaaagatgacagcattgataacaaagttttaactcgctcggacaaaaaccgcgggaaatctgataacgtcccgagcgaggaggtagtgaaaaagatgaaaaactattggcgccagctgctgaacgcgaaactcatcacccagcgtaaatttgataaccttacaaaagcagaacgtggtggtttgtccgagctggataaggcgggcttcatcaaacgccagctcgtagagacgcgccaaatcactaaacatgtggcgcaaatcctggatagccgtatgaatacgaagtacgatgagaatgacaaactgattcgcgaagtcaaagtgatcaccctgaaatctaagttggtgagcgattttcgcaaagactttcagttttataaggttcgcgagattaacaactatcatcatgcgcacgatgcctacttaaatgccgtggtgggcaccgctctcatcaaaaaatacccaaaattggagtcagagtttgtttacggtgattataaagtttatgatgttcgtaaaatgatcgcaaaaagcgagcaggagatcggaaaagcaacagccaagtattttttttatagcaatattatgaattttttcaaaacagaaatcaccttagcgaatggcgaaatccgtaagcgccctcttattgaaacaaatggggaaaccggcgaaattgtctgggataagggccgcgattttgccactgtgcgtaaagtgctgtctatgccacaggttaatatcgtgaaaaaaaccgaggtacagaccggtgggtttagtaaagaaagcattctgcccaagcgcaacagcgataaactgattgcgcgcaaaaaagattgggatccgaaaaaatatggtggcttcgattcgcctactgttgcgtattcggtcctcgtagtggctaaggtagaaaaaggtaaatctaaaaaattaaaaagcgtaaaagaattactgggaattacgattatggaacgtagctcgtttgagaaaaacccgattgacttcctggaagcgaaaggatataaagaagttaaaaaagatctgatcattaagctgccgaaatactcgctctttgaactcgaaaacggtcgtaaacgcatgctggcatcagcaggcgagctgcagaaaggcaacgagcttgcgttgccctccaaatacgtcaatttcttgtatctggctagtcattacgaaaaattgaaaggatccccggaagacaacgaacaaaaacaactgttcgtggaacagcacaaacattatcttgacgagatcattgaacagatctctgaattttccaaacgtgttattctggccgatgccaacctggacaaggttctttctgcgtacaacaaacatcgcgacaaaccaatccgcgagcaagctgaaaatattattcatttatttacactgacaaacctgggtgcgccggcggcgtttaaatatttcgatacaaccatcgatcgcaagcgttacacctctaccaaagaagtgctggatgccaccctgattcatcaaagcattacgggtctgtacgaaacgcgcattgatctgagtcagctgggcggggactaagtcgac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z