LOCUS BBa_K1795001 4476 bp DNA linear 23-Dec-2024 DEFINITION E. Coli Codon Optimized fdCAS9 under R0010 ACCESSION BBa_K1795001 VERSION BBa_K1795001.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..4339 /label=BBa_K1795000 stem_loop 4359..4402 /label=stem_loop misc_feature 4348..4427 /label=BBa_B0010 terminator 4348..4427 /label=BBa_B0010 stem_loop 4443..4462 /label=T7 TE misc_feature 4469..4469 /label=stop polyA_site 4463..4476 /label=polya misc_feature 4436..4476 /label=BBa_B0012 terminator 4436..4476 /label=BBa_B0012 terminator 4348..4476 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg acaaaaaata 241 cagtatcggc ctggcgatcg gcaccaattc agttggctgg gcagttatta ctgatgaata 301 caaggtcccc tccaaaaagt ttaaagtcct gggcaacacc gatcgtcaca gcatcaaaaa 361 aaatctgatc ggagccttgt tattcgacag tggtgagacg gcagaagcca cccgtttgaa 421 gcgtactgcc cgccgtcgct acacacgtcg taagaaccgt atctgttact tgcaggaaat 481 tttctcaaat gaaatggcga aagtggacga cagcttcttc caccgccttg aagagtcatt 541 cttagttgag gaagacaaaa aacacgaacg tcatcctatc tttggcaata tcgtcgatga 601 agtcgcgtat cacgaaaagt acccgaccat ttatcacttg cgtaaaaaac tggtcgactc 661 gaccgacaaa gcagacctgc gtctgattta tttagcattg gcccatatga ttaaatttcg 721 cggccacttc ttaatcgaag gcgacttgaa tccggacaac tcggatgtgg ataaattgtt 781 cattcagctg gtccagacgt ataatcagct ttttgaggaa aacccgatca acgcatcggg 841 cgttgatgcc aaggcgattt tatccgcacg cctgtcgaaa agccgccgtc tggaaaatct 901 catcgcacaa ctgccgggcg aaaagaaaaa cggtctgttc gggaacttga ttgcactgtc 961 cctgggcctg accccaaact ttaaaagcaa tttcgacttg gccgaagatg cgaagctgca 1021 gctgagtaaa gacacctatg atgacgactt agataatttg ttggcgcaga ttggtgatca 1081 atacgccgac ctctttctcg cagcgaaaaa tctgagtgat gcgatcttat tatctgatat 1141 cctgcgcgtc aacaccgaaa ttaccaaagc accgctgtcc gccagtatga tcaagcgcta 1201 cgacgagcat catcaagatc tgacccttct caaagcgtta gtgcgccagc agctgccgga 1261 aaaatacaaa gagatcttct tcgaccagag taaaaacgga tatgcaggtt acattgatgg 1321 cggcgcatca caggaagaat tttataaatt tattaaacca attttagaga aaatggatgg 1381 tactgaagaa ctccttgtca aactgaaccg cgaagatctt ctgcgcaaac aacgcacgtt 1441 tgacaacggt tccattccgc atcaaattca tttgggagaa ctccatgcta ttctgcgtcg 1501 tcaggaagat ttctatccgt ttttgaagga taatcgtgag aagattgaga aaatcctgac 1561 atttcgtatc ccttactatg tcggtcctct ggcgcgcggc aacagccgct ttgcatggat 1621 gactcgtaaa agcgaggaaa cgattacccc gtggaatttc gaagaagtag tggacaaagg 1681 agcgtcggcg cagagtttta ttgaacgcat gaccaatttc gataaaaatc tgccgaacga 1741 aaaagtactg ccaaaacact ctctgctgta tgaatacttc accgtgtaca atgaactcac 1801 taaggtcaaa tatgtaacag aaggtatgcg taaacccgcg tttttaagcg gggaacaaaa 1861 aaaggccatc gtagaccttc tcttcaaaac gaaccgcaag gtaaccgtga aacagttaaa 1921 agaggactat ttcaaaaaaa tcgaatgttt tgacagcgtg gagatctcag gtgtggaaga 1981 tcgcttcaac gcttcactcg gaacttacca tgatctgtta aagatcatta aagacaagga 2041 ttttcttgat aacgaagaga acgaagacat tttagaggat atcgtcctga cactgacttt 2101 gtttgaagat cgcgaaatga ttgaagaacg cctgaaaacc tatgcgcatc tgtttgatga 2161 caaagtcatg aaacagctga aacgtcgtcg ctatactggc tggggccgtc tgagtcgcaa 2221 actcattaac ggtattcgtg acaaacaaag tggtaaaacg attctggatt ttcttaaaag 2281 cgacggattt gcgaatcgta acttcatgca attaatccac gacgattcgc tgacatttaa 2341 agaggacatt caaaaagctc aggtgagtgg ccagggtgac agcttgcatg agcatattgc 2401 gaacttagct ggtagccctg ccattaaaaa aggcattttg cagactgtaa aagtggtgga 2461 cgaactggtt aaggtgatgg gccgccacaa accagaaaat atcgttattg aaatggcacg 2521 cgaaaaccag actacgcaaa aaggtcagaa aaactctcgt gaacgtatga aacgcatcga 2581 agaaggaatc aaagaactgg gtagccagat cctgaaagaa cacccggtgg aaaacacgca 2641 actgcagaac gaaaaactgt atctctatta tttacaaaat ggtcgcgaca tgtatgtgga 2701 tcaagaattg gatattaatc gcttgtccga ttatgacgtc gatgcgattg ttccgcagtc 2761 cttcctgaaa gatgacagca ttgataacaa agttttaact cgctcggaca aaaaccgcgg 2821 gaaatctgat aacgtcccga gcgaggaggt agtgaaaaag atgaaaaact attggcgcca 2881 gctgctgaac gcgaaactca tcacccagcg taaatttgat aaccttacaa aagcagaacg 2941 tggtggtttg tccgagctgg ataaggcggg cttcatcaaa cgccagctcg tagagacgcg 3001 ccaaatcact aaacatgtgg cgcaaatcct ggatagccgt atgaatacga agtacgatga 3061 gaatgacaaa ctgattcgcg aagtcaaagt gatcaccctg aaatctaagt tggtgagcga 3121 ttttcgcaaa gactttcagt tttataaggt tcgcgagatt aacaactatc atcatgcgca 3181 cgatgcctac ttaaatgccg tggtgggcac cgctctcatc aaaaaatacc caaaattgga 3241 gtcagagttt gtttacggtg attataaagt ttatgatgtt cgtaaaatga tcgcaaaaag 3301 cgagcaggag atcggaaaag caacagccaa gtattttttt tatagcaata ttatgaattt 3361 tttcaaaaca gaaatcacct tagcgaatgg cgaaatccgt aagcgccctc ttattgaaac 3421 aaatggggaa accggcgaaa ttgtctggga taagggccgc gattttgcca ctgtgcgtaa 3481 agtgctgtct atgccacagg ttaatatcgt gaaaaaaacc gaggtacaga ccggtgggtt 3541 tagtaaagaa agcattctgc ccaagcgcaa cagcgataaa ctgattgcgc gcaaaaaaga 3601 ttgggatccg aaaaaatatg gtggcttcga ttcgcctact gttgcgtatt cggtcctcgt 3661 agtggctaag gtagaaaaag gtaaatctaa aaaattaaaa agcgtaaaag aattactggg 3721 aattacgatt atggaacgta gctcgtttga gaaaaacccg attgacttcc tggaagcgaa 3781 aggatataaa gaagttaaaa aagatctgat cattaagctg ccgaaatact cgctctttga 3841 actcgaaaac ggtcgtaaac gcatgctggc atcagcaggc gagctgcaga aaggcaacga 3901 gcttgcgttg ccctccaaat acgtcaattt cttgtatctg gctagtcatt acgaaaaatt 3961 gaaaggatcc ccggaagaca acgaacaaaa acaactgttc gtggaacagc acaaacatta 4021 tcttgacgag atcattgaac agatctctga attttccaaa cgtgttattc tggccgatgc 4081 caacctggac aaggttcttt ctgcgtacaa caaacatcgc gacaaaccaa tccgcgagca 4141 agctgaaaat attattcatt tatttacact gacaaacctg ggtgcgccgg cggcgtttaa 4201 atatttcgat acaaccatcg atcgcaagcg ttacacctct accaaagaag tgctggatgc 4261 caccctgatt catcaaagca ttacgggtct gtacgaaacg cgcattgatc tgagtcagct 4321 gggcggggac taagtcgact actagagcca ggcatcaaat aaaacgaaag gctcagtcga 4381 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 4441 ctggctcacc ttcgggtggg cctttctgcg tttata //