BBa_K1795000
1
BBa_K1795000
E. Coli Codon Optimized dCAS9, solo
2015-09-16T11:00:00Z
2015-09-17T09:39:49Z
The basic template for the sgRNA sequence was taken from the supplementary information of Qi LS, Larson MH, Gilbert LA, et al. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013;152(5):1173-1183. doi:10.1016/j.cell.2013.02.022.
This sgRNA sequence was designed to target the Promoter Bba_R0010 with the N20 sequence atgttgtgtggaattgtgag. The part is under the control of the promoter Bba_J23100. If this gRNA is expressed in a cell with a form of the dCas9 protein, the protein will be targeted to the N20 sequence and repress gene expression. Expression of this part is repressed in the presence of LacI and CAP protein.
false
false
_2220_
27446
21426
9
false
Ensure the promoter targeted by this part is not used within this part.
false
Andrew D Halleran, Joseph L Maniaci
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961224
1
-35
range1961224
1
137
142
annotation1961227
1
start
range1961227
1
173
173
annotation1961225
1
-10
range1961225
1
161
166
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_K1795001
1
BBa_K1795001
E. Coli Codon Optimized fdCAS9 under R0010
2015-09-16T11:00:00Z
2015-09-17T09:39:24Z
Thanks to team iGEM13_SJTU-BioX-Shanghai for original, non-codon optimized dCas9 sequence.
This sequence codes for dCas9 and is codon optimized for E. Coli. It is under the promoter R0010 and has RBS B0034 and DT B0015.
false
false
_2220_
27446
27446
9
false
false
Andrew D Halleran, Joseph L Maniaci
component2467996
1
BBa_B0034
component2467997
1
BBa_K1795000
component2467988
1
BBa_R0010
component2468004
1
BBa_B0015
annotation2467988
1
BBa_R0010
range2467988
1
1
200
annotation2467997
1
BBa_K1795000
range2467997
1
227
4339
annotation2467996
1
BBa_B0034
range2467996
1
209
220
annotation2468004
1
BBa_B0015
range2468004
1
4348
4476
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1795001_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggacaaaaaatacagtatcggcctggcgatcggcaccaattcagttggctgggcagttattactgatgaatacaaggtcccctccaaaaagtttaaagtcctgggcaacaccgatcgtcacagcatcaaaaaaaatctgatcggagccttgttattcgacagtggtgagacggcagaagccacccgtttgaagcgtactgcccgccgtcgctacacacgtcgtaagaaccgtatctgttacttgcaggaaattttctcaaatgaaatggcgaaagtggacgacagcttcttccaccgccttgaagagtcattcttagttgaggaagacaaaaaacacgaacgtcatcctatctttggcaatatcgtcgatgaagtcgcgtatcacgaaaagtacccgaccatttatcacttgcgtaaaaaactggtcgactcgaccgacaaagcagacctgcgtctgatttatttagcattggcccatatgattaaatttcgcggccacttcttaatcgaaggcgacttgaatccggacaactcggatgtggataaattgttcattcagctggtccagacgtataatcagctttttgaggaaaacccgatcaacgcatcgggcgttgatgccaaggcgattttatccgcacgcctgtcgaaaagccgccgtctggaaaatctcatcgcacaactgccgggcgaaaagaaaaacggtctgttcgggaacttgattgcactgtccctgggcctgaccccaaactttaaaagcaatttcgacttggccgaagatgcgaagctgcagctgagtaaagacacctatgatgacgacttagataatttgttggcgcagattggtgatcaatacgccgacctctttctcgcagcgaaaaatctgagtgatgcgatcttattatctgatatcctgcgcgtcaacaccgaaattaccaaagcaccgctgtccgccagtatgatcaagcgctacgacgagcatcatcaagatctgacccttctcaaagcgttagtgcgccagcagctgccggaaaaatacaaagagatcttcttcgaccagagtaaaaacggatatgcaggttacattgatggcggcgcatcacaggaagaattttataaatttattaaaccaattttagagaaaatggatggtactgaagaactccttgtcaaactgaaccgcgaagatcttctgcgcaaacaacgcacgtttgacaacggttccattccgcatcaaattcatttgggagaactccatgctattctgcgtcgtcaggaagatttctatccgtttttgaaggataatcgtgagaagattgagaaaatcctgacatttcgtatcccttactatgtcggtcctctggcgcgcggcaacagccgctttgcatggatgactcgtaaaagcgaggaaacgattaccccgtggaatttcgaagaagtagtggacaaaggagcgtcggcgcagagttttattgaacgcatgaccaatttcgataaaaatctgccgaacgaaaaagtactgccaaaacactctctgctgtatgaatacttcaccgtgtacaatgaactcactaaggtcaaatatgtaacagaaggtatgcgtaaacccgcgtttttaagcggggaacaaaaaaaggccatcgtagaccttctcttcaaaacgaaccgcaaggtaaccgtgaaacagttaaaagaggactatttcaaaaaaatcgaatgttttgacagcgtggagatctcaggtgtggaagatcgcttcaacgcttcactcggaacttaccatgatctgttaaagatcattaaagacaaggattttcttgataacgaagagaacgaagacattttagaggatatcgtcctgacactgactttgtttgaagatcgcgaaatgattgaagaacgcctgaaaacctatgcgcatctgtttgatgacaaagtcatgaaacagctgaaacgtcgtcgctatactggctggggccgtctgagtcgcaaactcattaacggtattcgtgacaaacaaagtggtaaaacgattctggattttcttaaaagcgacggatttgcgaatcgtaacttcatgcaattaatccacgacgattcgctgacatttaaagaggacattcaaaaagctcaggtgagtggccagggtgacagcttgcatgagcatattgcgaacttagctggtagccctgccattaaaaaaggcattttgcagactgtaaaagtggtggacgaactggttaaggtgatgggccgccacaaaccagaaaatatcgttattgaaatggcacgcgaaaaccagactacgcaaaaaggtcagaaaaactctcgtgaacgtatgaaacgcatcgaagaaggaatcaaagaactgggtagccagatcctgaaagaacacccggtggaaaacacgcaactgcagaacgaaaaactgtatctctattatttacaaaatggtcgcgacatgtatgtggatcaagaattggatattaatcgcttgtccgattatgacgtcgatgcgattgttccgcagtccttcctgaaagatgacagcattgataacaaagttttaactcgctcggacaaaaaccgcgggaaatctgataacgtcccgagcgaggaggtagtgaaaaagatgaaaaactattggcgccagctgctgaacgcgaaactcatcacccagcgtaaatttgataaccttacaaaagcagaacgtggtggtttgtccgagctggataaggcgggcttcatcaaacgccagctcgtagagacgcgccaaatcactaaacatgtggcgcaaatcctggatagccgtatgaatacgaagtacgatgagaatgacaaactgattcgcgaagtcaaagtgatcaccctgaaatctaagttggtgagcgattttcgcaaagactttcagttttataaggttcgcgagattaacaactatcatcatgcgcacgatgcctacttaaatgccgtggtgggcaccgctctcatcaaaaaatacccaaaattggagtcagagtttgtttacggtgattataaagtttatgatgttcgtaaaatgatcgcaaaaagcgagcaggagatcggaaaagcaacagccaagtattttttttatagcaatattatgaattttttcaaaacagaaatcaccttagcgaatggcgaaatccgtaagcgccctcttattgaaacaaatggggaaaccggcgaaattgtctgggataagggccgcgattttgccactgtgcgtaaagtgctgtctatgccacaggttaatatcgtgaaaaaaaccgaggtacagaccggtgggtttagtaaagaaagcattctgcccaagcgcaacagcgataaactgattgcgcgcaaaaaagattgggatccgaaaaaatatggtggcttcgattcgcctactgttgcgtattcggtcctcgtagtggctaaggtagaaaaaggtaaatctaaaaaattaaaaagcgtaaaagaattactgggaattacgattatggaacgtagctcgtttgagaaaaacccgattgacttcctggaagcgaaaggatataaagaagttaaaaaagatctgatcattaagctgccgaaatactcgctctttgaactcgaaaacggtcgtaaacgcatgctggcatcagcaggcgagctgcagaaaggcaacgagcttgcgttgccctccaaatacgtcaatttcttgtatctggctagtcattacgaaaaattgaaaggatccccggaagacaacgaacaaaaacaactgttcgtggaacagcacaaacattatcttgacgagatcattgaacagatctctgaattttccaaacgtgttattctggccgatgccaacctggacaaggttctttctgcgtacaacaaacatcgcgacaaaccaatccgcgagcaagctgaaaatattattcatttatttacactgacaaacctgggtgcgccggcggcgtttaaatatttcgatacaaccatcgatcgcaagcgttacacctctaccaaagaagtgctggatgccaccctgattcatcaaagcattacgggtctgtacgaaacgcgcattgatctgagtcagctgggcggggactaagtcgactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1795000_sequence
1
atggacaaaaaatacagtatcggcctggcgatcggcaccaattcagttggctgggcagttattactgatgaatacaaggtcccctccaaaaagtttaaagtcctgggcaacaccgatcgtcacagcatcaaaaaaaatctgatcggagccttgttattcgacagtggtgagacggcagaagccacccgtttgaagcgtactgcccgccgtcgctacacacgtcgtaagaaccgtatctgttacttgcaggaaattttctcaaatgaaatggcgaaagtggacgacagcttcttccaccgccttgaagagtcattcttagttgaggaagacaaaaaacacgaacgtcatcctatctttggcaatatcgtcgatgaagtcgcgtatcacgaaaagtacccgaccatttatcacttgcgtaaaaaactggtcgactcgaccgacaaagcagacctgcgtctgatttatttagcattggcccatatgattaaatttcgcggccacttcttaatcgaaggcgacttgaatccggacaactcggatgtggataaattgttcattcagctggtccagacgtataatcagctttttgaggaaaacccgatcaacgcatcgggcgttgatgccaaggcgattttatccgcacgcctgtcgaaaagccgccgtctggaaaatctcatcgcacaactgccgggcgaaaagaaaaacggtctgttcgggaacttgattgcactgtccctgggcctgaccccaaactttaaaagcaatttcgacttggccgaagatgcgaagctgcagctgagtaaagacacctatgatgacgacttagataatttgttggcgcagattggtgatcaatacgccgacctctttctcgcagcgaaaaatctgagtgatgcgatcttattatctgatatcctgcgcgtcaacaccgaaattaccaaagcaccgctgtccgccagtatgatcaagcgctacgacgagcatcatcaagatctgacccttctcaaagcgttagtgcgccagcagctgccggaaaaatacaaagagatcttcttcgaccagagtaaaaacggatatgcaggttacattgatggcggcgcatcacaggaagaattttataaatttattaaaccaattttagagaaaatggatggtactgaagaactccttgtcaaactgaaccgcgaagatcttctgcgcaaacaacgcacgtttgacaacggttccattccgcatcaaattcatttgggagaactccatgctattctgcgtcgtcaggaagatttctatccgtttttgaaggataatcgtgagaagattgagaaaatcctgacatttcgtatcccttactatgtcggtcctctggcgcgcggcaacagccgctttgcatggatgactcgtaaaagcgaggaaacgattaccccgtggaatttcgaagaagtagtggacaaaggagcgtcggcgcagagttttattgaacgcatgaccaatttcgataaaaatctgccgaacgaaaaagtactgccaaaacactctctgctgtatgaatacttcaccgtgtacaatgaactcactaaggtcaaatatgtaacagaaggtatgcgtaaacccgcgtttttaagcggggaacaaaaaaaggccatcgtagaccttctcttcaaaacgaaccgcaaggtaaccgtgaaacagttaaaagaggactatttcaaaaaaatcgaatgttttgacagcgtggagatctcaggtgtggaagatcgcttcaacgcttcactcggaacttaccatgatctgttaaagatcattaaagacaaggattttcttgataacgaagagaacgaagacattttagaggatatcgtcctgacactgactttgtttgaagatcgcgaaatgattgaagaacgcctgaaaacctatgcgcatctgtttgatgacaaagtcatgaaacagctgaaacgtcgtcgctatactggctggggccgtctgagtcgcaaactcattaacggtattcgtgacaaacaaagtggtaaaacgattctggattttcttaaaagcgacggatttgcgaatcgtaacttcatgcaattaatccacgacgattcgctgacatttaaagaggacattcaaaaagctcaggtgagtggccagggtgacagcttgcatgagcatattgcgaacttagctggtagccctgccattaaaaaaggcattttgcagactgtaaaagtggtggacgaactggttaaggtgatgggccgccacaaaccagaaaatatcgttattgaaatggcacgcgaaaaccagactacgcaaaaaggtcagaaaaactctcgtgaacgtatgaaacgcatcgaagaaggaatcaaagaactgggtagccagatcctgaaagaacacccggtggaaaacacgcaactgcagaacgaaaaactgtatctctattatttacaaaatggtcgcgacatgtatgtggatcaagaattggatattaatcgcttgtccgattatgacgtcgatgcgattgttccgcagtccttcctgaaagatgacagcattgataacaaagttttaactcgctcggacaaaaaccgcgggaaatctgataacgtcccgagcgaggaggtagtgaaaaagatgaaaaactattggcgccagctgctgaacgcgaaactcatcacccagcgtaaatttgataaccttacaaaagcagaacgtggtggtttgtccgagctggataaggcgggcttcatcaaacgccagctcgtagagacgcgccaaatcactaaacatgtggcgcaaatcctggatagccgtatgaatacgaagtacgatgagaatgacaaactgattcgcgaagtcaaagtgatcaccctgaaatctaagttggtgagcgattttcgcaaagactttcagttttataaggttcgcgagattaacaactatcatcatgcgcacgatgcctacttaaatgccgtggtgggcaccgctctcatcaaaaaatacccaaaattggagtcagagtttgtttacggtgattataaagtttatgatgttcgtaaaatgatcgcaaaaagcgagcaggagatcggaaaagcaacagccaagtattttttttatagcaatattatgaattttttcaaaacagaaatcaccttagcgaatggcgaaatccgtaagcgccctcttattgaaacaaatggggaaaccggcgaaattgtctgggataagggccgcgattttgccactgtgcgtaaagtgctgtctatgccacaggttaatatcgtgaaaaaaaccgaggtacagaccggtgggtttagtaaagaaagcattctgcccaagcgcaacagcgataaactgattgcgcgcaaaaaagattgggatccgaaaaaatatggtggcttcgattcgcctactgttgcgtattcggtcctcgtagtggctaaggtagaaaaaggtaaatctaaaaaattaaaaagcgtaaaagaattactgggaattacgattatggaacgtagctcgtttgagaaaaacccgattgacttcctggaagcgaaaggatataaagaagttaaaaaagatctgatcattaagctgccgaaatactcgctctttgaactcgaaaacggtcgtaaacgcatgctggcatcagcaggcgagctgcagaaaggcaacgagcttgcgttgccctccaaatacgtcaatttcttgtatctggctagtcattacgaaaaattgaaaggatccccggaagacaacgaacaaaaacaactgttcgtggaacagcacaaacattatcttgacgagatcattgaacagatctctgaattttccaaacgtgttattctggccgatgccaacctggacaaggttctttctgcgtacaacaaacatcgcgacaaaccaatccgcgagcaagctgaaaatattattcatttatttacactgacaaacctgggtgcgccggcggcgtttaaatatttcgatacaaccatcgatcgcaagcgttacacctctaccaaagaagtgctggatgccaccctgattcatcaaagcattacgggtctgtacgaaacgcgcattgatctgagtcagctgggcggggactaagtcgac
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z