LOCUS BBa_K1795011 285 bp DNA linear 26-Nov-2024 DEFINITION I13453 gRNA under R0040 ACCESSION BBa_K1795011 VERSION BBa_K1795011.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 55..74 /label=BBa_K1795111 misc_feature 75..156 /label=BBa_K1795100 stem_loop 168..211 /label=stem_loop misc_feature 157..236 /label=BBa_B0010 terminator 157..236 /label=BBa_B0010 stem_loop 252..271 /label=T7 TE misc_feature 278..278 /label=stop polyA_site 272..285 /label=polya misc_feature 245..285 /label=BBa_B0012 terminator 245..285 /label=BBa_B0012 terminator 157..285 /label=BBa_B0015 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacgtttct 61 ccataccgtt ttttgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 121 caacttgaaa aagtggcacc gagtcggtgc ttttttccag gcatcaaata aaacgaaagg 181 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 241 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //