LOCUS       BBa_K1795011             285 bp    DNA     linear       28-Jun-2024
DEFINITION  I13453 gRNA under R0040
ACCESSION   BBa_K1795011
VERSION     BBa_K1795011.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    55..74
                     /label=BBa_K1795111
     misc_feature    75..156
                     /label=BBa_K1795100
     stem_loop       168..211
                     /label=stem_loop
     misc_feature    157..236
                     /label=BBa_B0010
     terminator      157..236
                     /label=BBa_B0010
     stem_loop       252..271
                     /label=T7 TE
     misc_feature    278..278
                     /label=stop
     polyA_site      272..285
                     /label=polya
     misc_feature    245..285
                     /label=BBa_B0012
     terminator      245..285
                     /label=BBa_B0012
     terminator      157..285
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacgtttct
       61 ccataccgtt ttttgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
      121 caacttgaaa aagtggcacc gagtcggtgc ttttttccag gcatcaaata aaacgaaagg
      181 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      241 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//