LOCUS BBa_K1795023 3349 bp DNA linear 22-Nov-2024 DEFINITION GalK Integrator Cassette ACCESSION BBa_K1795023 VERSION BBa_K1795023.1 FEATURES Location/Qualifiers misc_feature 1..2027 /label=BBa_K1795400 misc_feature 2028..2077 /label=BBa_K1795200 misc_feature 2078..2099 /label=BBa_K1795202 misc_feature 2100..2120 /label=BBa_K1795201 CDS 2121..2208 /label=end of LacI coding region (inactive) misc_feature 2121..2320 /label=BBa_R0010 misc_feature 2209..2246 /label=CAP binding site promoter 2257..2262 /label=-35 promoter 2281..2286 /label=-10 misc_feature 2286..2320 /label=LacI binding site misc_feature 2293..2293 /label=start promoter 2121..2320 /label=BBa_R0010 misc_feature 2333..2336 /label=conserved RBS 2329..2340 /label=BBa_B0034 CDS 2347..3162 /label=BBa_K1795300 stem_loop 3182..3225 /label=stem_loop misc_feature 3171..3250 /label=BBa_B0010 terminator 3171..3250 /label=BBa_B0010 stem_loop 3266..3285 /label=T7 TE misc_feature 3292..3292 /label=stop polyA_site 3286..3299 /label=polya misc_feature 3259..3299 /label=BBa_B0012 terminator 3259..3299 /label=BBa_B0012 terminator 3171..3299 /label=BBa_B0015 misc_feature 2121..3299 /label=BBa_K1795024 misc_feature 3300..3349 /label=BBa_K1795203 ORIGIN 1 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt 61 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 241 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc 301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct 1021 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg 1081 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt 1141 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat 1201 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc 1261 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca 1321 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct 1381 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat 1441 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt 1501 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac 1561 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa 1621 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat 1681 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt 1741 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt 1801 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta 1861 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac 1921 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag 1981 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctgttc atattgttca 2041 gcgacagctt gctgtacggc aggcaccagc tcttccggaa ttcgcggccg cttctagagt 2101 actagtagcg gccgctgcag caatacgcaa accgcctctc cccgcgcgtt ggccgattca 2161 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 2221 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 2281 tatgttgtgt ggaattgtga gcggataaca atttcacaca tactagagaa agaggagaaa 2341 tactagatga gccatattca acgggaaacg tcttgctcca ggccgcgatt aaattccaac 2401 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg 2461 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa 2521 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt 2581 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc 2641 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa 2701 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat 2761 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac 2821 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc 2881 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat 2941 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga 3001 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag 3061 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg 3121 aataaattgc agtttcattt gatgctcgat gagtttttct aatactagag ccaggcatca 3181 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 3241 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat 3301 tcttacactc cggattcgcg aaaatggata tcgctgactg cgcgcaaac //