BBa_K1795023
type: ComponentDefinition
persistent identity: BBa K1795023
display id: BBa_K1795023
version: 1
was generated by: iGEM to SBOL conversion
description: GalK Integrator Cassette
created: 2015-09-17T11:00:00Z
modified: 2015-09-17T11:34:17Z
mutable provenance: iGEM Parts Registry. Homologous regions were modeled after those used in Elowitz, M. B., Levine, A. J., Siggia, E. D., & Swain, P. S. (2002). Stochastic gene expression in a single cell. Science, 297(5584), 1183-1186.
owned by: james
owned by: myers
mutable description: We created a modified backbone that will allow users to quickly and easily create parts that can be integrated onto the E. coli genome. This backbone is designed as follows (where H1 refers to the first region homologous to the E. coli genome, and H2 refers to the second homologous region) In order to insert a part onto the genome, the user simply needs to put a part onto this backbone (between the prefix and suffix), and PCR up the region between (and including) H1 and H2. Users can put their parts onto this backbone with their preferred method of assembly. This part exhibits resistance to chloramphenicol and kanamycin. The parts integrated with this cassette will contain kanamycin resistance, and will be integrated in the galK region of the E. coli genome. Homologous regions modeled after those in Elowitz et al 2002.
discontinued: false
dominant: false
experience: experience/None
group u list: _2220_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Andrew Halleran, Joseph Maniaci, Caroline Golino, Taylor Jacobs, John Marken, Elli Cryan, Panya Vij, Michael LeFew
type: DnaRegion
role: partType/Plasmid Backbone
role: SO:0000755
component: BBa_K1795203
component: BBa_K1795024
component: BBa_K1795201
component: BBa_K1795202
component: BBa_K1795200
component: BBa_K1795400
sequence annotation: BBa_K1795400
sequence annotation: BBa_K1795200
sequence annotation: BBa_K1795202
sequence annotation: BBa_K1795201
sequence annotation: BBa_K1795024
sequence annotation: BBa_K1795203
sequence: https://synbiohub.org/public/igem/BBa_K1795023_sequence/1

BBa_K1795203
type: Component
persistent identity: component2469225
display id: component2469225
version: 1
top level: BBa_K1795023
definition: BBa_K1795203
access: public

BBa_K1795024
type: Component
persistent identity: component2469224
display id: component2469224
version: 1
top level: BBa_K1795023
definition: BBa_K1795024
access: public

BBa_K1795201
type: Component
persistent identity: component2469206
display id: component2469206
version: 1
top level: BBa_K1795023
definition: BBa_K1795201
access: public

BBa_K1795202
type: Component
persistent identity: component2469205
display id: component2469205
version: 1
top level: BBa_K1795023
definition: BBa_K1795202
access: public

BBa_K1795200
type: Component
persistent identity: component2469204
display id: component2469204
version: 1
top level: BBa_K1795023
definition: BBa_K1795200
access: public

BBa_K1795400
type: Component
persistent identity: component2469203
display id: component2469203
version: 1
top level: BBa_K1795023
definition: BBa_K1795400
access: public

BBa_K1795400
type: SequenceAnnotation
persistent identity: annotation2469203
display id: annotation2469203
version: 1
top level: BBa_K1795023
location: https://synbiohub.org/public/igem/BBa_K1795023/annotation2469203/range2469203/1
component: BBa_K1795400

https://synbiohub.org/public/igem/BBa_K1795023/annotation2469203/range2469203/1
type: Range
persistent identity: range2469203
display id: range2469203
version: 1
top level: BBa_K1795023
direction: direction/reverse
start: 1
end: 2027
orientation: inline

BBa_K1795200
type: SequenceAnnotation
persistent identity: annotation2469204
display id: annotation2469204
version: 1
top level: BBa_K1795023
location: https://synbiohub.org/public/igem/BBa_K1795023/annotation2469204/range2469204/1
component: BBa_K1795200

https://synbiohub.org/public/igem/BBa_K1795023/annotation2469204/range2469204/1
type: Range
persistent identity: range2469204
display id: range2469204
version: 1
top level: BBa_K1795023
direction: direction/reverse
start: 2028
end: 2077
orientation: inline

BBa_K1795202
type: SequenceAnnotation
persistent identity: annotation2469205
display id: annotation2469205
version: 1
top level: BBa_K1795023
location: https://synbiohub.org/public/igem/BBa_K1795023/annotation2469205/range2469205/1
component: BBa_K1795202

https://synbiohub.org/public/igem/BBa_K1795023/annotation2469205/range2469205/1
type: Range
persistent identity: range2469205
display id: range2469205
version: 1
top level: BBa_K1795023
direction: direction/reverse
start: 2078
end: 2099
orientation: inline

BBa_K1795201
type: SequenceAnnotation
persistent identity: annotation2469206
display id: annotation2469206
version: 1
top level: BBa_K1795023
location: https://synbiohub.org/public/igem/BBa_K1795023/annotation2469206/range2469206/1
component: BBa_K1795201

https://synbiohub.org/public/igem/BBa_K1795023/annotation2469206/range2469206/1
type: Range
persistent identity: range2469206
display id: range2469206
version: 1
top level: BBa_K1795023
direction: direction/reverse
start: 2100
end: 2120
orientation: inline

BBa_K1795024
type: SequenceAnnotation
persistent identity: annotation2469224
display id: annotation2469224
version: 1
top level: BBa_K1795023
location: https://synbiohub.org/public/igem/BBa_K1795023/annotation2469224/range2469224/1
component: BBa_K1795024

https://synbiohub.org/public/igem/BBa_K1795023/annotation2469224/range2469224/1
type: Range
persistent identity: range2469224
display id: range2469224
version: 1
top level: BBa_K1795023
direction: direction/reverse
start: 2121
end: 3299
orientation: inline

BBa_K1795203
type: SequenceAnnotation
persistent identity: annotation2469225
display id: annotation2469225
version: 1
top level: BBa_K1795023
location: https://synbiohub.org/public/igem/BBa_K1795023/annotation2469225/range2469225/1
component: BBa_K1795203

https://synbiohub.org/public/igem/BBa_K1795023/annotation2469225/range2469225/1
type: Range
persistent identity: range2469225
display id: range2469225
version: 1
top level: BBa_K1795023
direction: direction/reverse
start: 3300
end: 3349
orientation: inline

BBa_K1795200
type: ComponentDefinition
persistent identity: BBa K1795200
display id: BBa_K1795200
version: 1
was generated by: iGEM to SBOL conversion
description: H1 region GalK
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T11:28:06Z
mutable provenance: Sequence modeled after sequence used in Elowitz, M. B., Levine, A. J., Siggia, E. D., & Swain, P. S. (2002). Stochastic gene expression in a single cell. Science, 297(5584), 1183-1186.
owned by: james
owned by: myers
mutable description: Used in construction of K1795023
discontinued: false
dominant: false
experience: experience/None
group u list: _2220_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Andrew D Halleran
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1795200_sequence/1

BBa_K1795201
type: ComponentDefinition
persistent identity: BBa K1795201
display id: BBa_K1795201
version: 1
was generated by: iGEM to SBOL conversion
description: Suffix Temp WM
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T10:16:47Z
mutable provenance: GalK Homologous Sequence modeled after sequence used in Elowitz, M. B., Levine, A. J., Siggia, E. D., & Swain, P. S. (2002). Stochastic gene expression in a single cell. Science, 297(5584), 1183-1186.
owned by: james
owned by: myers
mutable description: Temp part Suffix + H2 region GalK Temp WM used in designing K1795022, H2 region sequence inspired by Elowits et al 2002
discontinued: false
dominant: false
experience: experience/None
group u list: _2220_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Joseph L Maniaci
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K1795201_sequence/1

BBa_K1795202
type: ComponentDefinition
persistent identity: BBa K1795202
display id: BBa_K1795202
version: 1
was generated by: iGEM to SBOL conversion
description: Prefix Temp WM ease of construction
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T10:32:03Z
mutable provenance: iGEM prefix
owned by: james
owned by: myers
mutable description: Just the iGEM prefix, used to show components for part K1795202
discontinued: false
dominant: false
group u list: _2220_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: iGEM prefix
star: false
creator: Joseph L Maniaci
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K1795202_sequence/1

BBa_K1795024
type: ComponentDefinition
persistent identity: BBa K1795024
display id: BBa_K1795024
version: 1
was generated by: iGEM to SBOL conversion
description: KanR Operon under R0010
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T10:39:32Z
mutable provenance: iGEM Parts Registry
owned by: james
owned by: myers
mutable description: Provides Kanamycin Resistance to the cell under the Promoter R0010.
discontinued: false
dominant: false
group u list: _2220_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: N/a
star: false
creator: Joseph L Maniaci
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K1795300
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K1795300
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1795024_sequence/1

BBa_B0015
type: Component
persistent identity: component2468814
display id: component2468814
version: 1
top level: BBa_K1795024
definition: BBa_B0015
access: public

BBa_K1795300
type: Component
persistent identity: component2468807
display id: component2468807
version: 1
top level: BBa_K1795024
definition: BBa_K1795300
access: public

BBa_B0034
type: Component
persistent identity: component2468806
display id: component2468806
version: 1
top level: BBa_K1795024
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component2468798
display id: component2468798
version: 1
top level: BBa_K1795024
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2468798
display id: annotation2468798
version: 1
top level: BBa_K1795024
location: https://synbiohub.org/public/igem/BBa_K1795024/annotation2468798/range2468798/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1795024/annotation2468798/range2468798/1
type: Range
persistent identity: range2468798
display id: range2468798
version: 1
top level: BBa_K1795024
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2468806
display id: annotation2468806
version: 1
top level: BBa_K1795024
location: https://synbiohub.org/public/igem/BBa_K1795024/annotation2468806/range2468806/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1795024/annotation2468806/range2468806/1
type: Range
persistent identity: range2468806
display id: range2468806
version: 1
top level: BBa_K1795024
direction: direction/reverse
start: 209
end: 220
orientation: inline

BBa_K1795300
type: SequenceAnnotation
persistent identity: annotation2468807
display id: annotation2468807
version: 1
top level: BBa_K1795024
location: https://synbiohub.org/public/igem/BBa_K1795024/annotation2468807/range2468807/1
component: BBa_K1795300

https://synbiohub.org/public/igem/BBa_K1795024/annotation2468807/range2468807/1
type: Range
persistent identity: range2468807
display id: range2468807
version: 1
top level: BBa_K1795024
direction: direction/reverse
start: 227
end: 1042
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2468814
display id: annotation2468814
version: 1
top level: BBa_K1795024
location: https://synbiohub.org/public/igem/BBa_K1795024/annotation2468814/range2468814/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1795024/annotation2468814/range2468814/1
type: Range
persistent identity: range2468814
display id: range2468814
version: 1
top level: BBa_K1795024
direction: direction/reverse
start: 1051
end: 1179
orientation: inline

BBa_K1795203
type: ComponentDefinition
persistent identity: BBa K1795203
display id: BBa_K1795203
version: 1
was generated by: iGEM to SBOL conversion
description: H2 region GalK
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T10:56:05Z
mutable provenance: GalK Homologous Sequence modeled after sequence used in Elowitz, M. B., Levine, A. J., Siggia, E. D., & Swain, P. S. (2002). Stochastic gene expression in a single cell. Science, 297(5584), 1183-1186.
owned by: james
owned by: myers
mutable description: GalK homologous region H2: Based off of Elowitz et al 2002, Used in construction of K1795023
discontinued: false
dominant: false
group u list: _2220_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Joseph L Maniaci
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1795203_sequence/1

BBa_K1795400
type: ComponentDefinition
persistent identity: BBa K1795400
display id: BBa_K1795400
version: 1
was generated by: iGEM to SBOL conversion
description: Truncated pSB1C3 with no Prefix or Suffix
created: 2015-09-17T11:00:00Z
modified: 2015-09-17T11:04:43Z
mutable provenance: iGEM Parts Registry
owned by: james
owned by: myers
mutable description: This part is a truncated version of pSB1C3 which does not contain a prefix or suffix. It is made to be used as a construction intermediate for the creation of new plasmid backbones which want to insert additional regions at either end of the backbone before replacing the prefix and suffix at either end of the backbone. It is used in K1795023.
discontinued: false
dominant: false
group u list: _2220_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We removed the prefix and suffix from pSB1C3 so we could add additional regions on either end of the backbone before replacing the prefix and suffix.
star: false
creator: Joseph L Maniaci
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K1795400_sequence/1

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1795300
type: ComponentDefinition
persistent identity: BBa K1795300
display id: BBa_K1795300
version: 1
was generated by: iGEM to SBOL conversion
description: KanR coding region
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T10:49:41Z
mutable provenance: iGEM Parts Registry
owned by: james
owned by: myers
mutable description: Forward KanR Protein Coding region only. Needs promoter, RBSs, and Terminator in order to provide Kan resistance.
discontinued: false
dominant: false
experience: experience/None
group u list: _2220_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Had to flip an existing parts direction to get this sequence
star: false
creator: Joseph L Maniaci
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1795300_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1795023_sequence/1
type: Sequence
persistent identity: BBa K1795023 sequence
display id: BBa_K1795023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1795200_sequence/1
type: Sequence
persistent identity: BBa K1795200 sequence
display id: BBa_K1795200_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttcatattgttcagcgacagcttgctgtacggcaggcaccagctcttccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1795201_sequence/1
type: Sequence
persistent identity: BBa K1795201 sequence
display id: BBa_K1795201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1795202_sequence/1
type: Sequence
persistent identity: BBa K1795202 sequence
display id: BBa_K1795202_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1795024_sequence/1
type: Sequence
persistent identity: BBa K1795024 sequence
display id: BBa_K1795024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgagccatattcaacgggaaacgtcttgctccaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1795203_sequence/1
type: Sequence
persistent identity: BBa K1795203 sequence
display id: BBa_K1795203_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttcttacactccggattcgcgaaaatggatatcgctgactgcgcgcaaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1795400_sequence/1
type: Sequence
persistent identity: BBa K1795400 sequence
display id: BBa_K1795400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1795300_sequence/1
type: Sequence
persistent identity: BBa K1795300 sequence
display id: BBa_K1795300_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagccatattcaacgggaaacgtcttgctccaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z