BBa_K1796008 1 BBa_K1796008 nifD promoted from Paenibacillus sp. WLY78 2015-09-16T11:00:00Z 2015-09-17T08:19:32Z NifD(BBa_K1796008) is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it. Function: αsubunits of dinitrogease which also called FeMo protein. NifD encode a metallocluster: FeMo-co, a [Mo-7Fe-9S-C-homocitrate] cluster which serves as the active site of substrate binding and reduction. We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But we were not informed of the error in promotion.After several times failed expriments, we found the problem.We tackled them over the matter, mistakes were corrected, but the correct genes can't arrive on time,it can only arrive after the deadline. Sequence submit is promoted by the synthesis company, containing a PstI in the gene. The sequence we promoted: CAGGAGGGAGGAATAGGCCAAATGTCTTCTATCGTTGACAAAGGTAAACAGATCGTTGAAGAAATCCTGGAAGTTTACCCGAAAAAAGCTAAAAAAGACCGTACCAAACACTTCGAAATCGCTGACGAAGAACTGGTTAACTGCGGTACCTGCTCTATCAAATCTAACATGAAATCTCGTCCGGGTGTTATGACCGCTCGTGGTTGCGCTTACGCTGGTTCTAAAGGTGTTGTTTGGGGTCCGATCAAAGACATGGTTCACATCTCTCACGGTCCGATCGGTTGCGGTCAGTACTCTTGGGGTACCCGTCGTAACTACGCTAACGGTATCCTGGGTATCGACAACTTCACCGCTATGCAGATCACCTCTAACTTCCAGGAAAAAGACATCGTTTTCGGTGGTGACAAAAAACTGGAAGTTATCTGCCGTGAAATCAAAGAAATGTTCCCGCTGGCTAAAGGTATCTCTGTTCAGTCTGAATGCCCGGTTGGTCTGATCGGTGACGACATCGGTGCTGTTGCTAAAAAAATGACCGAAGAACTGGGTATCCCGGTTATCCCGGTTCGTTGCGAAGGTTTCCGTGGTGTTTCTCAGTCTCTGGGTCACCACATCGCTAACGACGCTATCCGTGACTTCCTGATGGGTCGTCGTGAACTGAAAGAATGCGGTCCGTACGACGTTTCTATCATCGGTGACTACAACATCGGTGGTGACGCTTGGGCTTCTCGTATCCTGCTGGAAGAAATGGGTCTGCGTGTTATCGCTCAGTGGTCTGGTGACGGTACCATCAACGAACTGGGTATCGCTCACAAATCTAAACTGAACCTGATCCACTGCCACCGTTCTATGAACTACATGTGCACCACCATGGAACAGGAATACGGTATCCCGTGGATGGAATACAACTTCTTCGGTCCGACCAAAACCATGGAATCTCTGCGTGCTATCGCTGCTCGTTTCGACGAAACCATCCAGGAAAAATGCGAACAGGTTATCGCTCAGTACATGCCGCAGATGGAAGCTGTTATCCGTAAATACCGTCCGCGTCTGGAAGGTAAAAAAGTTATGCTGCTGATCGGTGGTCTGCGTGCTCGTCACACCATCGGTGCTTACGAAGACCTGGGTATGGAAATCGTTGCTACCGGTTACGAATTTGCTCACAAAGACGACTACGAAAAAACCTTCCCGGACGTTAAAGAAGGTACCATCCTGTACGACGACCCGACCGCTTACGAACTGGAAGAACTGGCTCAGCGTCTGAACATCGACCTGATGGGTGCTGGTGTTAAAGAAAAATACGTTTACCACAAAATGGGTATCCCGTTCCGTCAGATGCACTCTTGGGACTACTCTGGTCCATACCACGGGTTTGACGGCTTCAAGATCTTCGCGCGTGACATGGACATGACCATCAACTCTCCGGTTTGGTCTCTGCTGCCGTCTCGTCAGACCGCTGAAGTTCCGGTTTAATAA false false _2221_ 23592 23592 9 false We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But the part can be assembled by gibson assembly,that is what we did. false Nannan Xie annotation2466009 1 nifD coding sequence range2466009 1 22 1473 annotation2475048 1 unknown rbs range2475048 1 1 21 BBa_K1796008_sequence 1 caggagggaggaataggccaaatgagcagcatcgtcgacaaaggcaaacagatcgtggaagaaatcctggaagtctacccgaaaaaagcgaaaaaagaccgcaccaaacacttcgagatcgcggacgaagaactggttaactgcggtacctgcagcatcaaaagcaacatgaaaagtcgtccgggcgttatgacggcacgtggttgtgcatacgcaggttcaaaaggcgttgtttggggtccgatcaaagacatggtccatattagccacggtccgattggttgcggtcaatattcttggggtacccgtcgtaactacgcaaacggtatcctgggcatcgataacttcaccgcaatgcagatcaccagcaacttccaggaaaaagacatcgtcttcggcggcgacaaaaaactggaagtcatctgccgcgaaatcaaagagatgtttccgctggcgaaaggcattagcgttcaatctgagtgtccggttggtctgattggcgacgatattggcgcggtcgccaaaaagatgaccgaagaactgggcattccggttattccggttcgttgcgaaggttttcgcggggttagtcagagcctgggtcatcatattgcaaacgacgcaattcgcgattttctgatgggccgtcgcgaactgaaagagtgcggtccgtacgacgttagcatcattggcgattacaacattggcggcgatgcttgggcaagtcgtattctgctggaagaaatgggtctgcgcgttattgcacagtggtctggcgacggtaccattaacgaactgggtatcgcgcacaaaagcaaactgaacctgatccactgccatcgttccatgaactacatgtgcaccaccatggaacaggaatacggtatcccgtggatggaatacaacttcttcggtccgaccaaaaccatggaaagcctgcgcgcaattgcagcgcgttttgacgaaaccatccaggagaaatgcgagcaggttattgcccagtatatgccgcagatggaggcggtcattcgtaaatatcgcccgcgtctggagggcaaaaaagttatgctgctgattggcggtctgcgcgcacgtcataccattggcgcatacgaagatctgggcatggaaattgttgcgaccggttacgaatttgcgcacaaagacgactacgagaaaaccttcccggacgtcaaagagggcaccattctgtacgacgatccgaccgcatacgaactggaagaactggcacagcgtctgaacattgatctgatgggcgcaggcgtcaaagagaaatacgtctaccacaaaatgggcattccgttccgtcagatgcatagctgggattatagcggtccgtatcacggtttcgacggctttaaaatcttcgcccgcgatatggatatgaccatcaacagcccggtttggagtctgctgccgtctcgtcaaaccgcagaagttccggtttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z