BBa_K1796011
1
BBa_K1796011
nifN promoted from Paenibacillus sp. WLY78
2015-09-16T11:00:00Z
2015-09-17T08:46:04Z
NifN is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it.
Function: scaffold gene of nitrogen fixation system
false
false
_2221_
23592
23592
9
false
We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves(done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut sites were involved(three PstI) after they promoted it again. But the part can be assembled by gibson assembly,that is what we did.
false
Nannan Xie
annotation2473704
1
nifN coding sequence
range2473704
1
22
1434
annotation2475143
1
unknown rbs
range2475143
1
1
21
BBa_K1796011_sequence
1
ggcacaaagcctatgcgggctatgaccggttgttgtctgtggcgtaactccctgtgcattcgttgtagcatccagtacggttcttaaccgccgaattggctgcacggtcgtcgtcgtcgtaatctgctgatcaaaagcgcaaccaaaccggtcagcgttaatccgctgaaagttggtcaaccgctgggcggcgttctggctctgcaaggtatgtatcgttctatgccgctgctgcatggcgcacaaggttgttctgcgtttagtaaagcactgctgacccgtcattttcgcgaaccgattgcagttcaaacctctgctctgcaggaaatggacgtcatcttcgacgcggatcgtaatctggaagaagcgctggatcacatctggagcaaacatcatccggacgttattggcgttattagcaccgcactgaccgaagttgcaggcgtagattttcagagccgcgttaaagcattcaaacgcgaacgcgcactgaaagatagcctgctgtttagcgttagcctgccggattttcatggttctctggaaaccggctatagcagtaccgtcgaatctctgatggacgcagttctgggtctggcaggcggtaaaagtccgaaaaaacagcgtcgtacccaggttaatctgctgccggcaagttatctgaccgctggcgacgttatggagatcaaagacatcatcgcgagctttggcctggaagttatcaccctgccggatattagcaccagtctgtcaggtcatctgctgaccggttttagtccgctgacccgcggcggtaccccgctggattctgcttgtcagatgctggaaagcagctgtaccattgcgattggcgcaagtatggaacgtccggcacgtcgtctgacccacgctgcaggtattccgtatcacctgtttgcaggtctgtctggtctggcagcatctgacgcatttatccacttcctgcagaaaattagccgcgaaccggcaccggtacgttttcgttggcaacgcgaaaatctgctggatagcatgctggacgcgcatttttactatagcggcgcaagcgcggttgtagcactggaaccggatcatatgctgtctaccgcagcttggctggaagaaatgggggttgagctgaaacgtctgattaccccgtgtagtaccccggcactgcaaaaaaccgaacgcgaagtttggattggcgacctggacgacgcagaagaatctgcacaaggcgttgatctgtggatctctaatagtcacggtcgtaaaggcgcagctcgtgcaggtgcaagttttgttccggcaggtctgccggtatacgacgaactgggcgcacatacctctgtttctgttggttatcgcggtaccatggagtgggtcaacaaagtaggcaacgtcctgctggctgaacgcggtcgcggcggttaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z