BBa_K1796014 1 BBa_K1796014 nifV promoted from Paenibacillus sp. WLY78 2015-09-16T11:00:00Z 2015-09-17T09:03:19Z It is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it. homocitrate synthase，provides the organic moiety in all three nitrogenase cofactors. First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But we were not informed of the error in promotion.After several times failed expriments, we found the problem.We tackled them over the matter, mistakes were corrected, but the correct genes can't arrive on time,it can only arrive after the deadline. Sequence submit is promoted by the synthesis company, containing a PstI in the gene. false false _2221_ 23592 23592 9 false We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves.Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But the part can be assembled by gibson assembly,that is what we did. false Nannan Xie annotation2475151 1 unkown rbs range2475151 1 1 21 annotation2473746 1 nifV range2473746 1 22 1161 BBa_K1796014_sequence 1 gcggaacgggaggggatacagatgagccgtctgcatatttgcgataccaccctgcgtgacggcgaacaagcaccgggcgttgcgtttagcgcggaagagaaaaccgaaatcgcgattatgctggatagcgcaggcgttgaacaggcagaaattggcattccggcaatgggtaaaaccgagtgccgtagcattgcacgcattgcagctctgggtctgcaaatgaaactgatgacctggaaccgcgcagtttttaccgatatcgatgcaaccgaaagtaccggcgtaggttgggcacatattagcgttccggttagcaccgttcagatgaaatccaaactgggcatgaacccggaacaggttaccgaactgatccgcaaaagcgttgactacgcactgtgcaaaggtctgaccgttagcgttggttttgaagacgcaagtcgcgcagacgacctgtttctggaacaactggcaaaccagctgtatcgcgacggtattcgtcgttttcgctacgcagataccctgagcgttcatcatccggcagcaattgcagcacgtattgatcgtctggtttctcgcgttccgcaagacgttgaactggagatccactgccacaacgattacggtctggcactggcaaataccctggcagcactgcaagcaggcgcagtttgggcaagtaccaccgtttctggtctgggcgaacgcgcaggtaataccgcactggaagaggttgtcatgagctggcgcgatctgtatcagggtacctgttctgttcgtccggaactgctgaatccgctggcagcactggttagcaaagcgagcaatcgcattatcccggaaggtaaaccgattgtcggcgatatggtatttgcgcacgaaagcggcatccatattaacggtctgctgaaagaacgcgcagcatatcaagcactggatccgaccgaactgggtaccgatcatagctttgtcctgggcaaacactctggtcgttctgctgtccagtatatgctggaacaggaaggtattgaagcaggttctggcgagatcaaattcctgctggaacgtctgcgtctggttggcgaagatccgaaacgcgttatccattctgcagatctgcgtcgttggctgcaatattatccggcagaactgccgaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z