BBa_K1796001 1 Pnif promoter of Paenibacillus sp. WLY78 nif gene cluster 2015-09-16T11:00:00Z 2015-09-18T02:35:42Z Pnif is from Paenibacillus sp. WLY78 genome,located upstream of nifB,the first gene of nitrogen fixation cluster. The promoter of Paenibacillus sp. WLY78 nif gene cluster, We named it Pnif. Pnif is a &#963;70-dependent promoter located upstream of nifB. Pnif is in control of all the gene clusters of nitrogen fixation genome. Confirmation of nif promoter We standardized the nif promoter of Paenibacillus sp. WLY78, and linked it to the upstream of 3 reporter genes (RFP reporter: BBa_K1357010, YFP reporter: BBa_E0430;amilCP reporter: BBa_K1357009) to confirm the reliability of this promoter. The results are shown in Figure 2. A B C http://2015.igem.org/wiki/images/b/b2/Confirmation_of_Pnif_AB.png http://2015.igem.org/wiki/images/thumb/3/34/C-confirmation_of_Pnif.jpeg/622px-C-confirmation_of_Pnif.jpeg Fig.4. Confirmation of nif promoter of Paenibacillus sp. WLY78. (A) Pnif+RFP reporter; (B) Pnif+YFP reporter;(C)Pnif+amilCP reporter. The results confirmed the reliability of Pnif. The initiation of Pnif does not require inducement. Thus it is a effective constitutive promoter. false false _2221_ 12637 23592 9 false effeciency of the promoter in E.coli for ordinary parts false Nannan Xie annotation2473637 1 -35 range2473637 1 62 67 annotation2473660 1 -10 range2473660 1 85 90 annotation2473661 1 Pnif range2473661 1 1 100 BBa_K1796012 1 BBa_K1796012 nifX promoted from Paenibacillus sp. WLY78 2015-09-16T11:00:00Z 2015-09-17T08:52:34Z NifX(BBa_K1796012) is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it. Function: encode carrier proteins involved in the delivery of FeMoco to the MoFe protein , participate in FeMoco assembly in a chaperone-like function. false false _2221_ 23592 23592 9 false We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves&#65288;done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site&#65288;one PstI&#65289; was involved after they promoted it again. But the part can be assembled by gibson assembly,that is what we did. false Nannan Xie annotation2473705 1 nifX coding sequence range2473705 1 22 414 annotation2475144 1 unknown rbs range2475144 1 1 21 BBa_K1796006 1 BBa_K1796006 Dinitrogenase reductase(nifH) 2015-09-14T11:00:00Z 2015-09-18T07:21:59Z NifH(BBa_K1796006) is taken from genomic sequence of Paenibacillus sp. WLY78, we apply promotions on the sequences and synthesised it. Dinitrogenase reductase, important in nitrogen fixation. false false _2221_ 12637 23592 9 false We put it in a pET-28b plasmid to express it. false Nannan Xie annotation2474986 1 unknown rbs range2474986 1 1 21 annotation2456188 1 nifH range2456188 1 22 891 BBa_K1796010 1 BBa_K1796010 nifE promoted from Paenibacillus sp. WLY78 2015-09-16T11:00:00Z 2015-09-17T08:39:40Z NifE(BBa_K1796010) is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it. Function: scaffold false false _2221_ 23592 23592 9 false We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselvesThen we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But the part can be assembled by gibson assembly,that is what we did. false Nannan Xie annotation2475102 1 unknown rbs range2475102 1 1 21 annotation2473683 1 nifE range2473683 1 22 1386 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K1796015 1 BBa_K1796015 complete line of nif cluster 2015-09-17T11:00:00Z 2015-09-18T09:54:28Z Paenibacillus sp.,promoted whole line of nif cluster,contain Pnif,nifBHDKENXV,hesA false false _2221_ 23592 23592 9 false whole line of nif cluster false Nannan Xie component2475717 1 BBa_K1796001 component2475744 1 BBa_K1796014 component2475735 1 BBa_K1796011 component2475729 1 BBa_K1796009 component2475738 1 BBa_K1796012 component2475751 1 BBa_B0015 component2475732 1 BBa_K1796010 component2475741 1 BBa_K1796013 component2475720 1 BBa_K1796006 component2475723 1 BBa_K1796007 component2475726 1 BBa_K1796008 annotation2475732 1 BBa_K1796010 range2475732 1 5583 6968 annotation2475741 1 BBa_K1796013 range2475741 1 8841 9629 annotation2475720 1 BBa_K1796006 range2475720 1 109 999 annotation2475726 1 BBa_K1796008 range2475726 1 2540 4012 annotation2475717 1 BBa_K1796001 range2475717 1 1 100 annotation2475723 1 BBa_K1796007 range2475723 1 1008 2531 annotation2475744 1 BBa_K1796014 range2475744 1 9638 10798 annotation2475729 1 BBa_K1796009 range2475729 1 4021 5574 annotation2475751 1 BBa_B0015 range2475751 1 10807 10935 annotation2475735 1 BBa_K1796011 range2475735 1 6977 8410 annotation2475738 1 BBa_K1796012 range2475738 1 8419 8832 BBa_K1796014 1 BBa_K1796014 nifV promoted from Paenibacillus sp. WLY78 2015-09-16T11:00:00Z 2015-09-17T09:03:19Z It is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it. homocitrate synthase&#65292;provides the organic moiety in all three nitrogenase cofactors. First, we promoted it ourselves&#65288;done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But we were not informed of the error in promotion.After several times failed expriments, we found the problem.We tackled them over the matter, mistakes were corrected, but the correct genes can't arrive on time,it can only arrive after the deadline. Sequence submit is promoted by the synthesis company, containing a PstI in the gene. false false _2221_ 23592 23592 9 false We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves.Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But the part can be assembled by gibson assembly,that is what we did. false Nannan Xie annotation2475151 1 unkown rbs range2475151 1 1 21 annotation2473746 1 nifV range2473746 1 22 1161 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_K1796011 1 BBa_K1796011 nifN promoted from Paenibacillus sp. WLY78 2015-09-16T11:00:00Z 2015-09-17T08:46:04Z NifN is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it. Function: scaffold gene of nitrogen fixation system false false _2221_ 23592 23592 9 false We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves&#65288;done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut sites were involved(three PstI) after they promoted it again. But the part can be assembled by gibson assembly,that is what we did. false Nannan Xie annotation2473704 1 nifN coding sequence range2473704 1 22 1434 annotation2475143 1 unknown rbs range2475143 1 1 21 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1796008 1 BBa_K1796008 nifD promoted from Paenibacillus sp. WLY78 2015-09-16T11:00:00Z 2015-09-17T08:19:32Z NifD(BBa_K1796008) is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it. Function: &#945;subunits of dinitrogease which also called FeMo protein. NifD encode a metallocluster: FeMo-co, a [Mo-7Fe-9S-C-homocitrate] cluster which serves as the active site of substrate binding and reduction. We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves&#65288;done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But we were not informed of the error in promotion.After several times failed expriments, we found the problem.We tackled them over the matter, mistakes were corrected, but the correct genes can't arrive on time,it can only arrive after the deadline. Sequence submit is promoted by the synthesis company, containing a PstI in the gene. The sequence we promoted: CAGGAGGGAGGAATAGGCCAAATGTCTTCTATCGTTGACAAAGGTAAACAGATCGTTGAAGAAATCCTGGAAGTTTACCCGAAAAAAGCTAAAAAAGACCGTACCAAACACTTCGAAATCGCTGACGAAGAACTGGTTAACTGCGGTACCTGCTCTATCAAATCTAACATGAAATCTCGTCCGGGTGTTATGACCGCTCGTGGTTGCGCTTACGCTGGTTCTAAAGGTGTTGTTTGGGGTCCGATCAAAGACATGGTTCACATCTCTCACGGTCCGATCGGTTGCGGTCAGTACTCTTGGGGTACCCGTCGTAACTACGCTAACGGTATCCTGGGTATCGACAACTTCACCGCTATGCAGATCACCTCTAACTTCCAGGAAAAAGACATCGTTTTCGGTGGTGACAAAAAACTGGAAGTTATCTGCCGTGAAATCAAAGAAATGTTCCCGCTGGCTAAAGGTATCTCTGTTCAGTCTGAATGCCCGGTTGGTCTGATCGGTGACGACATCGGTGCTGTTGCTAAAAAAATGACCGAAGAACTGGGTATCCCGGTTATCCCGGTTCGTTGCGAAGGTTTCCGTGGTGTTTCTCAGTCTCTGGGTCACCACATCGCTAACGACGCTATCCGTGACTTCCTGATGGGTCGTCGTGAACTGAAAGAATGCGGTCCGTACGACGTTTCTATCATCGGTGACTACAACATCGGTGGTGACGCTTGGGCTTCTCGTATCCTGCTGGAAGAAATGGGTCTGCGTGTTATCGCTCAGTGGTCTGGTGACGGTACCATCAACGAACTGGGTATCGCTCACAAATCTAAACTGAACCTGATCCACTGCCACCGTTCTATGAACTACATGTGCACCACCATGGAACAGGAATACGGTATCCCGTGGATGGAATACAACTTCTTCGGTCCGACCAAAACCATGGAATCTCTGCGTGCTATCGCTGCTCGTTTCGACGAAACCATCCAGGAAAAATGCGAACAGGTTATCGCTCAGTACATGCCGCAGATGGAAGCTGTTATCCGTAAATACCGTCCGCGTCTGGAAGGTAAAAAAGTTATGCTGCTGATCGGTGGTCTGCGTGCTCGTCACACCATCGGTGCTTACGAAGACCTGGGTATGGAAATCGTTGCTACCGGTTACGAATTTGCTCACAAAGACGACTACGAAAAAACCTTCCCGGACGTTAAAGAAGGTACCATCCTGTACGACGACCCGACCGCTTACGAACTGGAAGAACTGGCTCAGCGTCTGAACATCGACCTGATGGGTGCTGGTGTTAAAGAAAAATACGTTTACCACAAAATGGGTATCCCGTTCCGTCAGATGCACTCTTGGGACTACTCTGGTCCATACCACGGGTTTGACGGCTTCAAGATCTTCGCGCGTGACATGGACATGACCATCAACTCTCCGGTTTGGTCTCTGCTGCCGTCTCGTCAGACCGCTGAAGTTCCGGTTTAATAA false false _2221_ 23592 23592 9 false We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves&#65288;done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But the part can be assembled by gibson assembly,that is what we did. false Nannan Xie annotation2466009 1 nifD coding sequence range2466009 1 22 1473 annotation2475048 1 unknown rbs range2475048 1 1 21 BBa_K1796009 1 BBa_K1796009 nifK promoted from Paenibacillus sp. WLY78 2015-09-16T11:00:00Z 2015-09-17T08:42:28Z NifK(BBa_K1796009) is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it. Function: &#946; subunits of dinitrogease which also called FeMo protein.NifK encode a metallocluster: the P-cluster, a [8Fe-7S] cluster which shuttles electrons to FeMo-co. We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves&#65288;done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis. false false _2221_ 23592 23592 9 false We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves&#65288;done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis,the part can be assembled by gibson assembly,that is what we did. false Nannan Xie annotation2473682 1 nifK coding sequence range2473682 1 22 1554 annotation2475079 1 uknown rbs range2475079 1 1 21 BBa_K1796013 1 BBa_K1796013 hesA promoted from Paenibacillus sp. WLY78 2015-09-16T11:00:00Z 2015-09-17T08:57:35Z It is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it. Function has not so far been determined , but Deletion analysis indicates that in addition to the core nif genes, hesA plays an important role in nitrogen fixation and is responsive to the availability of molybdenum. HesA a member of the ThiF-MoeB-HesA family and contains an N-terminal nucleotide binding domain and a C-terminal MoeZ/MoeB-like domain. The ThiF-MoeB-HesA family engage in an ATP-dependent process that activates the C-terminus of partner ubiquitin-like proteins by forming an acyl adenylate complex that facilitates sulfur transfer. First, we promoted it ourselves&#65288;done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But we were not informed of the error in promotion.After several times failed expriments, we found the problem.We tackled them over the matter, mistakes were corrected, but the correct genes can't arrive on time,it can only arrive after the deadline. Sequence submit is promoted by the synthesis company, containing a PstI in the gene false false _2221_ 23592 23592 9 false We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves&#65288;done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But the part can be assembled by gibson assembly,that is what we did. false Nannan Xie annotation2475145 1 unknown rbs range2475145 1 1 21 annotation2473742 1 hesA coding sequence range2473742 1 22 789 BBa_K1796007 1 BBa_K1796007 nifB from Paenibacillus sp. WLY78 2015-09-16T11:00:00Z 2015-09-18T02:58:06Z NifB(BBa_K1796007) is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it. essential for biosynthesis of the active-site nitrogenase cofactor and encodes a radical A-adenosylmethionine(SAM)-dependent enzyme that inserts the central carbon atom into the eight-Fe core of nifB cofactor(nifB-co). false false _2221_ 12637 23592 9 false The part contains a sequence of 21bp possible RBS region false Nannan Xie annotation2473679 1 nifB coding seqquence range2473679 1 22 1524 annotation2475008 1 unknown rbs range2475008 1 1 21 BBa_K1796008_sequence 1 caggagggaggaataggccaaatgagcagcatcgtcgacaaaggcaaacagatcgtggaagaaatcctggaagtctacccgaaaaaagcgaaaaaagaccgcaccaaacacttcgagatcgcggacgaagaactggttaactgcggtacctgcagcatcaaaagcaacatgaaaagtcgtccgggcgttatgacggcacgtggttgtgcatacgcaggttcaaaaggcgttgtttggggtccgatcaaagacatggtccatattagccacggtccgattggttgcggtcaatattcttggggtacccgtcgtaactacgcaaacggtatcctgggcatcgataacttcaccgcaatgcagatcaccagcaacttccaggaaaaagacatcgtcttcggcggcgacaaaaaactggaagtcatctgccgcgaaatcaaagagatgtttccgctggcgaaaggcattagcgttcaatctgagtgtccggttggtctgattggcgacgatattggcgcggtcgccaaaaagatgaccgaagaactgggcattccggttattccggttcgttgcgaaggttttcgcggggttagtcagagcctgggtcatcatattgcaaacgacgcaattcgcgattttctgatgggccgtcgcgaactgaaagagtgcggtccgtacgacgttagcatcattggcgattacaacattggcggcgatgcttgggcaagtcgtattctgctggaagaaatgggtctgcgcgttattgcacagtggtctggcgacggtaccattaacgaactgggtatcgcgcacaaaagcaaactgaacctgatccactgccatcgttccatgaactacatgtgcaccaccatggaacaggaatacggtatcccgtggatggaatacaacttcttcggtccgaccaaaaccatggaaagcctgcgcgcaattgcagcgcgttttgacgaaaccatccaggagaaatgcgagcaggttattgcccagtatatgccgcagatggaggcggtcattcgtaaatatcgcccgcgtctggagggcaaaaaagttatgctgctgattggcggtctgcgcgcacgtcataccattggcgcatacgaagatctgggcatggaaattgttgcgaccggttacgaatttgcgcacaaagacgactacgagaaaaccttcccggacgtcaaagagggcaccattctgtacgacgatccgaccgcatacgaactggaagaactggcacagcgtctgaacattgatctgatgggcgcaggcgtcaaagagaaatacgtctaccacaaaatgggcattccgttccgtcagatgcatagctgggattatagcggtccgtatcacggtttcgacggctttaaaatcttcgcccgcgatatggatatgaccatcaacagcccggtttggagtctgctgccgtctcgtcaaaccgcagaagttccggtttaataa BBa_K1796010_sequence 1 caggagggaggaataggccaaatggaaccggcagttagtaacggtcgtctggaagtcagctgcggcaacaaaatcccgaaatctaccccgtgtccgcgtccggttccgggtgaagcatctggcggttgttcttttgacggtgcacagatcaccctgattccgattgcagatgcagcacatctggttcacggtccgattgcttgtctgggtaacagttgggaaagtcgcggtagtctgagttctggtccggaactgtctgcatacggttttaccaccgatctgggcgaacaagacatcatcttcggcagcgaacagaaactgcacgaaagcatccgctatattgtcagccgttttgcaccgccggccgtttttgtttataccacctgtgttaccgcactgaccggcgaagatattgaaggcgtctgcaaagcggaaagcgaacgtctgggtaccccgattattccggttaacagtccgggctttgtcggtagcaaaaatctgggtacccgtctggcaggcgacgttctgtttcagcatatcatcggtagtaccgaaccggaacagaccaccagtcacgatatcaacctgatcggcgaatacaatatcgccggcgaaatgtggcatatcgaacgtctgatgcaacaggcaggtatgagcattctgagccgtattaccggcgatggtcgttttcgcgaagttggttgggcacatcgtgccaaagcgaacatggtcgtttgtagtcgcgcactgctgggtctggctgttcaaatggaacgcaaatacggcatcccgtacttcgaaggtagcttttacggcgcaaaagaaaccagctattccctgcgccaaatggcatatctgaccggcgatcgcgacgttgaacgccgcgttgataaactggctgctcgcgaagaaatgcgtctgagtctggaactggaaccgtatcgcaaacagctgaaaggcaaacgcgcagttctgtataccggcggcgttaaaagctggtctgttattaccgcgctgcaggaactgggtattaaagtcgttggcgttggtaccaataaatctaccgcggaagatgttagccgtattgcggatcgtattggcgacgatgcagaatatattccggaaggcggtgcacgtcaaattctgaaaaccgttcgcagccgcaaagcagatatggttattgctggcggtcgcaacatgtacatggcactgaaagagcagatcccgtttgtcgacatcaaccaggaacgtcataaagcgtacgctggttacgacggtctgctgagtctggcaaaacagctggttcataccctgcaacatccggtttgggaactgaccgcaaaactggcaccgtgggaagaagaaaccgaattcgcagattaataa BBa_K1796014_sequence 1 gcggaacgggaggggatacagatgagccgtctgcatatttgcgataccaccctgcgtgacggcgaacaagcaccgggcgttgcgtttagcgcggaagagaaaaccgaaatcgcgattatgctggatagcgcaggcgttgaacaggcagaaattggcattccggcaatgggtaaaaccgagtgccgtagcattgcacgcattgcagctctgggtctgcaaatgaaactgatgacctggaaccgcgcagtttttaccgatatcgatgcaaccgaaagtaccggcgtaggttgggcacatattagcgttccggttagcaccgttcagatgaaatccaaactgggcatgaacccggaacaggttaccgaactgatccgcaaaagcgttgactacgcactgtgcaaaggtctgaccgttagcgttggttttgaagacgcaagtcgcgcagacgacctgtttctggaacaactggcaaaccagctgtatcgcgacggtattcgtcgttttcgctacgcagataccctgagcgttcatcatccggcagcaattgcagcacgtattgatcgtctggtttctcgcgttccgcaagacgttgaactggagatccactgccacaacgattacggtctggcactggcaaataccctggcagcactgcaagcaggcgcagtttgggcaagtaccaccgtttctggtctgggcgaacgcgcaggtaataccgcactggaagaggttgtcatgagctggcgcgatctgtatcagggtacctgttctgttcgtccggaactgctgaatccgctggcagcactggttagcaaagcgagcaatcgcattatcccggaaggtaaaccgattgtcggcgatatggtatttgcgcacgaaagcggcatccatattaacggtctgctgaaagaacgcgcagcatatcaagcactggatccgaccgaactgggtaccgatcatagctttgtcctgggcaaacactctggtcgttctgctgtccagtatatgctggaacaggaaggtattgaagcaggttctggcgagatcaaattcctgctggaacgtctgcgtctggttggcgaagatccgaaacgcgttatccattctgcagatctgcgtcgttggctgcaatattatccggcagaactgccgaaataataa BBa_K1796009_sequence 1 gcagactgcggaggtgccggtatgagcgaacgtccgaacatcgttgatcacaaccagctgttccgccaggataaatacgtccgtcaacgcgaagagaaacgcgcttttgaagcaccgtgtagtccggaagaagttaccgataccctggagtacaccaaaaccaaagagtacaaagacaaaaactttgcgcgcaccgcggttgttgttaatccggcaaaagcctgtcaaccgttaggcgcagttatggcagcactgggttttgagaaaaccctgccgtttattcacggctctcagggttgtaccgcatattttcgcagccatctggcacgtcatttcaaagaaccggttccggctgtttctaccagtatgacggaagacgcagctgtttttggcggtatgcgtaacctgatcgacggcattgaaaactgcattgcgctgtaccagccggaaatgattgcagtttgcaccacctgcatggcagaagttattggcgacgatctgagcgcatttctggcaaacgcacgtcaagaaggcgttttaccggaagatatgccggttccgtttgcaaacaccccgagttttagcggtagccatatcaccggctacgacgcaatgttacgtagcgtcctggaaaccctgtacaacaaaagcggtcgtaccgcacaaccgggtcacgaattaaaactgaacgtcctgctgggctttgacggttataccggtaacttcgcggaaatgcgtcgtatgctgggtatgtttggggcaacctataccatcctgggcgatcacagcagcaactttgattctggcgcgaccggcgaatatagctattattacggtggtaccccgttagaagacgttccgaaagcagcagacgcagcaggtaccttagcaattcagcagtacagcctgcgtaaaaccctgggctatatgaaacagacctggggtcagcaagttagcagcatttctaccccgctgggtattcgcgcaaccgatcgcttactggaagaaattagccgtttaagcggtcgcgaaattccggaagcactgaaacaggaacgcgcacgtattgttgacgctatgatggatagccacgcatatctgcacggtaaacgcgttgcaatggcaggcgatccggatatgttaatcggtctgatcggcttctgcttagaactgggcatggaaccggtacatatcgtttgcagcaacggcgatcgcaaattcgagaaagaagcggaactgctgctgaaatcctctccgtatggcgcagaagcaaccgttcattctggtcaggatctgtggcatatgcgtagcctgctgtttcaagatccggttgatctggctattggtagcagccatctgaaatttgcggcgaaagaggcggaaattccgttactgcgcgttggttttccgatctttgatcgccatcacctgcaccgttatccgattattggctatcagggcgcactgaatctgctgacccagtttgtcaacaccatcctggacgtcatggaagaacaagcaccggatcatagctttgacttagttcgttaataa BBa_K1796001_sequence 1 cgtaaaatttgacacatatgtgaattgaggataaatgtcagggatttcatggagaagtgaattgactgtatttgtccctgtctctaagatgtaattatat BBa_K1796013_sequence 1 gcacatggagggggtggacgaatggtgcaactgctggaagatagccgttacggtcgtcaactgaaactgctgggcgttgaaggtcagaatcgtctgaaacaagccaccgttatggttgcaggtattggcggtctgggcggcgctgcagcaatgtatctggctgctgctggtgttggtaaactgattctggctcacgaaggcgttatccatctgccggatatgaaccgtcaggttctgatggatagcggtcgtattggcgaagaacgtatggaaaccgcactgcaacatctgcatcgcattaacccggaaaccgaactggaaggtcacgcacatcgtattaccgaagaaagctctggtccgtgggttgaagcatctgatatcgtcatcgacgcacgttacgattttccggaacgttacgctctgaatcgtctgtgcgttcgtcacggtcgtccgatgattgaagcagcaatgtacgcgtacgaagtcagcctgatgaccattgatccggggaaaaccgcttgtctggagtgtctgtatccggaaggcggtcaaccgtgggaaccgctgggttttccggtactgggcgcaacctctggtctgattggttgtatggcagcactggaagccgttaaatggattaccgacgcaggtaacctgttcaccgatcgtatgtaccgcatgaacgtcctggatatgagcagctgtaccatcgcagtcaaacgtaatccgcgttgtccgtgttgtggtaccggcggcgataccgacgaaagcgtggcatatctgtaataa BBa_K1796012_sequence 1 tgccgagagggggaggggaggatgaaagttgcgtttgcaaccgaagacggcgttctggtaaacgcacattttggccagagcccgatgtttaccatcttcgaaatccgccactctggcgttcagtttctggaacatcgtcgtattgcgctgggttctgacgaaaacgaagcaggcaaaatcgcgagccgtattggtctgattgaagactgcgcgctgatctttctggttcaaattggcgcaagcgcagcagcacaagttaccaaacgcaccatcatgccggtcaaagttgcatttggcagcaccattgaagaacaggtacagcgtctgcagaatatgctgacccgtaatccgccgatgtggctggcaaaaattctgcacgcagaagaaggttctggtaaagcagaaagctaataa BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1796006_sequence 1 cattttggaggctgagaaaatatgcgtcagatcgctttctacggtaaaggtggtatcggtaaatctaccacctctcagaacaccctggctcagctggctaccaaattcaaacagaaaatcatgatcgttggttgcgacccgaaagctgactctacccgtctgatcctgaacaccaaagctcagcagaccgttctgcacctggctgctgaacgtggtaccgttgaagacctggaactggaagacgttgttcagaaaggtttcggtgacatcctgaacgttgaatgcggtggtccggaaccgggtgttggttgcgctggtcgtggtatcatcaccgctatcaacttcctggaagaagaaggtgcttacgaaggtctggacttcgtttcttacgacgttctgggtgacgttgtttgcggtggtttcgctatgccgatccgtgaaaaaaaagctcaggaaatctacatcgtttgctctggtgaaatgatggctatgtacgctgctaacaacatcgctcgtggtatcctgaaatacgctaactctggtggtgttcgtctgggtggtctgatctgcaactctcgtaacaccgacctggaagctgaactgatcaccgaactggctcgtcgtctgaacacccagatgatccacttcctgccgcgtgacaacgttgttcagcacgctgaactgcgtcgtatgaccgttacccagtacaacccggaacacaaacaggctgctgaatacgaagaactggctggtaaaatcctgaacaacgacatgctgaccgttccgaccccgatctctatggaagacctggaagacctgctgatggaatttggtatcatcgaagacgaagaaaccgctatcaacaaagctgaagcttctggtcagtaataa BBa_K1796011_sequence 1 ggcacaaagcctatgcgggctatgaccggttgttgtctgtggcgtaactccctgtgcattcgttgtagcatccagtacggttcttaaccgccgaattggctgcacggtcgtcgtcgtcgtaatctgctgatcaaaagcgcaaccaaaccggtcagcgttaatccgctgaaagttggtcaaccgctgggcggcgttctggctctgcaaggtatgtatcgttctatgccgctgctgcatggcgcacaaggttgttctgcgtttagtaaagcactgctgacccgtcattttcgcgaaccgattgcagttcaaacctctgctctgcaggaaatggacgtcatcttcgacgcggatcgtaatctggaagaagcgctggatcacatctggagcaaacatcatccggacgttattggcgttattagcaccgcactgaccgaagttgcaggcgtagattttcagagccgcgttaaagcattcaaacgcgaacgcgcactgaaagatagcctgctgtttagcgttagcctgccggattttcatggttctctggaaaccggctatagcagtaccgtcgaatctctgatggacgcagttctgggtctggcaggcggtaaaagtccgaaaaaacagcgtcgtacccaggttaatctgctgccggcaagttatctgaccgctggcgacgttatggagatcaaagacatcatcgcgagctttggcctggaagttatcaccctgccggatattagcaccagtctgtcaggtcatctgctgaccggttttagtccgctgacccgcggcggtaccccgctggattctgcttgtcagatgctggaaagcagctgtaccattgcgattggcgcaagtatggaacgtccggcacgtcgtctgacccacgctgcaggtattccgtatcacctgtttgcaggtctgtctggtctggcagcatctgacgcatttatccacttcctgcagaaaattagccgcgaaccggcaccggtacgttttcgttggcaacgcgaaaatctgctggatagcatgctggacgcgcatttttactatagcggcgcaagcgcggttgtagcactggaaccggatcatatgctgtctaccgcagcttggctggaagaaatgggggttgagctgaaacgtctgattaccccgtgtagtaccccggcactgcaaaaaaccgaacgcgaagtttggattggcgacctggacgacgcagaagaatctgcacaaggcgttgatctgtggatctctaatagtcacggtcgtaaaggcgcagctcgtgcaggtgcaagttttgttccggcaggtctgccggtatacgacgaactgggcgcacatacctctgtttctgttggttatcgcggtaccatggagtgggtcaacaaagtaggcaacgtcctgctggctgaacgcggtcgcggcggttaataa BBa_K1796007_sequence 1 agagaacgtagagaggagggaatggatagcttagcggatctgagcgaaaccccgttagcattagaaaccctgcgtcgtcatccgtgttataacgaagaagcgcatcgctactttgcgcgtattcatttaccggttgcaccggcttgcaacattcagtgccactactgcaaccgcaaattcgactgcgttaacgaaagccgtccgggcgttgtttctgaattattaaccccggaacaggcagcaagtaaaacctacggcgttgctgcccaactgatgcagttaagcgttgttggtattgcaggtccgggcgatccgttagcaaacgcggaagcgacctttgatacctttcgtcgcgttcgcgaaaccgttaaagacgttatcttttgcctgagcaccaacggtctgaccttaattcgccacatcgatcgcatcgttgaactgggtattagccacgtcaccatcaccatcaacgcagttgatccggttgttggtagtcgcatttacggttgggtttacgacgaaggcaaacgttatgcaggcgaagaagcagcacgtttactgattgaccgtcagctggctggtctgaaaatgttagcatctcgcggcgttctgtgcaaagttaacagcgttctgatcccggaagttaacgacgcacatttaccggaagttgcacgcgtagttaaagaacacggcgcagttctgcacaacatcatgccgctgattattgcaccgggttctcgttacgaacaagaaggtatgcgcgcaccgcgtccgcgtttagttcgtcagttacaggaacagtgcgcagaagctggcgctgttattatgcgtcattgtcgtcagtgtcgcgcagacgcaattggtttactgggcgaagatcgtaaccaggattttacctgggaaaatattgcggcagctccgccgatggacgaagaagcacgcgcacaatttcagaaagagctggacgaaaaagtccgcgttcgtatggaacgtaaagaaggccagagccatcataaacaaccgagtaccggcgcaggttgtagttgtccgttaagcggcgataaaccggaagcaagcttcaccagcaaaccggttctgattgcagttgcaagtcgcggcggcggtaaagttaatcagcatttcggccgcgccaaagaattcatgatctacgaaagcgacggcaccattgtcaacttcatcggcattcgcaaagtccagagctactgtcacggtaaagcggattgtaacggcgacaaagcggagaccatcaaagagatcctgagcatggttcacgattgcgcactgttactgagctctggtattggcgaagcaccgaaagaagccttacaggaagctggcgttttaccgattgtttgcggcggcgatattgaagaaagcgttctggagtacgtcaaattcctgcgctacatgtatccggttcagaccggtaaaggcagcaaacgtaacaaaggcgtcaaaggtaaccacagcgatctgccgattgaacattttgggggttaataa BBa_K1796015_sequence 1 cgtaaaatttgacacatatgtgaattgaggataaatgtcagggatttcatggagaagtgaattgactgtatttgtccctgtctctaagatgtaattatattactagagcattttggaggctgagaaaatatgcgtcagatcgctttctacggtaaaggtggtatcggtaaatctaccacctctcagaacaccctggctcagctggctaccaaattcaaacagaaaatcatgatcgttggttgcgacccgaaagctgactctacccgtctgatcctgaacaccaaagctcagcagaccgttctgcacctggctgctgaacgtggtaccgttgaagacctggaactggaagacgttgttcagaaaggtttcggtgacatcctgaacgttgaatgcggtggtccggaaccgggtgttggttgcgctggtcgtggtatcatcaccgctatcaacttcctggaagaagaaggtgcttacgaaggtctggacttcgtttcttacgacgttctgggtgacgttgtttgcggtggtttcgctatgccgatccgtgaaaaaaaagctcaggaaatctacatcgtttgctctggtgaaatgatggctatgtacgctgctaacaacatcgctcgtggtatcctgaaatacgctaactctggtggtgttcgtctgggtggtctgatctgcaactctcgtaacaccgacctggaagctgaactgatcaccgaactggctcgtcgtctgaacacccagatgatccacttcctgccgcgtgacaacgttgttcagcacgctgaactgcgtcgtatgaccgttacccagtacaacccggaacacaaacaggctgctgaatacgaagaactggctggtaaaatcctgaacaacgacatgctgaccgttccgaccccgatctctatggaagacctggaagacctgctgatggaatttggtatcatcgaagacgaagaaaccgctatcaacaaagctgaagcttctggtcagtaataatactagagagagaacgtagagaggagggaatggatagcttagcggatctgagcgaaaccccgttagcattagaaaccctgcgtcgtcatccgtgttataacgaagaagcgcatcgctactttgcgcgtattcatttaccggttgcaccggcttgcaacattcagtgccactactgcaaccgcaaattcgactgcgttaacgaaagccgtccgggcgttgtttctgaattattaaccccggaacaggcagcaagtaaaacctacggcgttgctgcccaactgatgcagttaagcgttgttggtattgcaggtccgggcgatccgttagcaaacgcggaagcgacctttgatacctttcgtcgcgttcgcgaaaccgttaaagacgttatcttttgcctgagcaccaacggtctgaccttaattcgccacatcgatcgcatcgttgaactgggtattagccacgtcaccatcaccatcaacgcagttgatccggttgttggtagtcgcatttacggttgggtttacgacgaaggcaaacgttatgcaggcgaagaagcagcacgtttactgattgaccgtcagctggctggtctgaaaatgttagcatctcgcggcgttctgtgcaaagttaacagcgttctgatcccggaagttaacgacgcacatttaccggaagttgcacgcgtagttaaagaacacggcgcagttctgcacaacatcatgccgctgattattgcaccgggttctcgttacgaacaagaaggtatgcgcgcaccgcgtccgcgtttagttcgtcagttacaggaacagtgcgcagaagctggcgctgttattatgcgtcattgtcgtcagtgtcgcgcagacgcaattggtttactgggcgaagatcgtaaccaggattttacctgggaaaatattgcggcagctccgccgatggacgaagaagcacgcgcacaatttcagaaagagctggacgaaaaagtccgcgttcgtatggaacgtaaagaaggccagagccatcataaacaaccgagtaccggcgcaggttgtagttgtccgttaagcggcgataaaccggaagcaagcttcaccagcaaaccggttctgattgcagttgcaagtcgcggcggcggtaaagttaatcagcatttcggccgcgccaaagaattcatgatctacgaaagcgacggcaccattgtcaacttcatcggcattcgcaaagtccagagctactgtcacggtaaagcggattgtaacggcgacaaagcggagaccatcaaagagatcctgagcatggttcacgattgcgcactgttactgagctctggtattggcgaagcaccgaaagaagccttacaggaagctggcgttttaccgattgtttgcggcggcgatattgaagaaagcgttctggagtacgtcaaattcctgcgctacatgtatccggttcagaccggtaaaggcagcaaacgtaacaaaggcgtcaaaggtaaccacagcgatctgccgattgaacattttgggggttaataatactagagcaggagggaggaataggccaaatgagcagcatcgtcgacaaaggcaaacagatcgtggaagaaatcctggaagtctacccgaaaaaagcgaaaaaagaccgcaccaaacacttcgagatcgcggacgaagaactggttaactgcggtacctgcagcatcaaaagcaacatgaaaagtcgtccgggcgttatgacggcacgtggttgtgcatacgcaggttcaaaaggcgttgtttggggtccgatcaaagacatggtccatattagccacggtccgattggttgcggtcaatattcttggggtacccgtcgtaactacgcaaacggtatcctgggcatcgataacttcaccgcaatgcagatcaccagcaacttccaggaaaaagacatcgtcttcggcggcgacaaaaaactggaagtcatctgccgcgaaatcaaagagatgtttccgctggcgaaaggcattagcgttcaatctgagtgtccggttggtctgattggcgacgatattggcgcggtcgccaaaaagatgaccgaagaactgggcattccggttattccggttcgttgcgaaggttttcgcggggttagtcagagcctgggtcatcatattgcaaacgacgcaattcgcgattttctgatgggccgtcgcgaactgaaagagtgcggtccgtacgacgttagcatcattggcgattacaacattggcggcgatgcttgggcaagtcgtattctgctggaagaaatgggtctgcgcgttattgcacagtggtctggcgacggtaccattaacgaactgggtatcgcgcacaaaagcaaactgaacctgatccactgccatcgttccatgaactacatgtgcaccaccatggaacaggaatacggtatcccgtggatggaatacaacttcttcggtccgaccaaaaccatggaaagcctgcgcgcaattgcagcgcgttttgacgaaaccatccaggagaaatgcgagcaggttattgcccagtatatgccgcagatggaggcggtcattcgtaaatatcgcccgcgtctggagggcaaaaaagttatgctgctgattggcggtctgcgcgcacgtcataccattggcgcatacgaagatctgggcatggaaattgttgcgaccggttacgaatttgcgcacaaagacgactacgagaaaaccttcccggacgtcaaagagggcaccattctgtacgacgatccgaccgcatacgaactggaagaactggcacagcgtctgaacattgatctgatgggcgcaggcgtcaaagagaaatacgtctaccacaaaatgggcattccgttccgtcagatgcatagctgggattatagcggtccgtatcacggtttcgacggctttaaaatcttcgcccgcgatatggatatgaccatcaacagcccggtttggagtctgctgccgtctcgtcaaaccgcagaagttccggtttaataatactagaggcagactgcggaggtgccggtatgagcgaacgtccgaacatcgttgatcacaaccagctgttccgccaggataaatacgtccgtcaacgcgaagagaaacgcgcttttgaagcaccgtgtagtccggaagaagttaccgataccctggagtacaccaaaaccaaagagtacaaagacaaaaactttgcgcgcaccgcggttgttgttaatccggcaaaagcctgtcaaccgttaggcgcagttatggcagcactgggttttgagaaaaccctgccgtttattcacggctctcagggttgtaccgcatattttcgcagccatctggcacgtcatttcaaagaaccggttccggctgtttctaccagtatgacggaagacgcagctgtttttggcggtatgcgtaacctgatcgacggcattgaaaactgcattgcgctgtaccagccggaaatgattgcagtttgcaccacctgcatggcagaagttattggcgacgatctgagcgcatttctggcaaacgcacgtcaagaaggcgttttaccggaagatatgccggttccgtttgcaaacaccccgagttttagcggtagccatatcaccggctacgacgcaatgttacgtagcgtcctggaaaccctgtacaacaaaagcggtcgtaccgcacaaccgggtcacgaattaaaactgaacgtcctgctgggctttgacggttataccggtaacttcgcggaaatgcgtcgtatgctgggtatgtttggggcaacctataccatcctgggcgatcacagcagcaactttgattctggcgcgaccggcgaatatagctattattacggtggtaccccgttagaagacgttccgaaagcagcagacgcagcaggtaccttagcaattcagcagtacagcctgcgtaaaaccctgggctatatgaaacagacctggggtcagcaagttagcagcatttctaccccgctgggtattcgcgcaaccgatcgcttactggaagaaattagccgtttaagcggtcgcgaaattccggaagcactgaaacaggaacgcgcacgtattgttgacgctatgatggatagccacgcatatctgcacggtaaacgcgttgcaatggcaggcgatccggatatgttaatcggtctgatcggcttctgcttagaactgggcatggaaccggtacatatcgtttgcagcaacggcgatcgcaaattcgagaaagaagcggaactgctgctgaaatcctctccgtatggcgcagaagcaaccgttcattctggtcaggatctgtggcatatgcgtagcctgctgtttcaagatccggttgatctggctattggtagcagccatctgaaatttgcggcgaaagaggcggaaattccgttactgcgcgttggttttccgatctttgatcgccatcacctgcaccgttatccgattattggctatcagggcgcactgaatctgctgacccagtttgtcaacaccatcctggacgtcatggaagaacaagcaccggatcatagctttgacttagttcgttaataatactagagcaggagggaggaataggccaaatggaaccggcagttagtaacggtcgtctggaagtcagctgcggcaacaaaatcccgaaatctaccccgtgtccgcgtccggttccgggtgaagcatctggcggttgttcttttgacggtgcacagatcaccctgattccgattgcagatgcagcacatctggttcacggtccgattgcttgtctgggtaacagttgggaaagtcgcggtagtctgagttctggtccggaactgtctgcatacggttttaccaccgatctgggcgaacaagacatcatcttcggcagcgaacagaaactgcacgaaagcatccgctatattgtcagccgttttgcaccgccggccgtttttgtttataccacctgtgttaccgcactgaccggcgaagatattgaaggcgtctgcaaagcggaaagcgaacgtctgggtaccccgattattccggttaacagtccgggctttgtcggtagcaaaaatctgggtacccgtctggcaggcgacgttctgtttcagcatatcatcggtagtaccgaaccggaacagaccaccagtcacgatatcaacctgatcggcgaatacaatatcgccggcgaaatgtggcatatcgaacgtctgatgcaacaggcaggtatgagcattctgagccgtattaccggcgatggtcgttttcgcgaagttggttgggcacatcgtgccaaagcgaacatggtcgtttgtagtcgcgcactgctgggtctggctgttcaaatggaacgcaaatacggcatcccgtacttcgaaggtagcttttacggcgcaaaagaaaccagctattccctgcgccaaatggcatatctgaccggcgatcgcgacgttgaacgccgcgttgataaactggctgctcgcgaagaaatgcgtctgagtctggaactggaaccgtatcgcaaacagctgaaaggcaaacgcgcagttctgtataccggcggcgttaaaagctggtctgttattaccgcgctgcaggaactgggtattaaagtcgttggcgttggtaccaataaatctaccgcggaagatgttagccgtattgcggatcgtattggcgacgatgcagaatatattccggaaggcggtgcacgtcaaattctgaaaaccgttcgcagccgcaaagcagatatggttattgctggcggtcgcaacatgtacatggcactgaaagagcagatcccgtttgtcgacatcaaccaggaacgtcataaagcgtacgctggttacgacggtctgctgagtctggcaaaacagctggttcataccctgcaacatccggtttgggaactgaccgcaaaactggcaccgtgggaagaagaaaccgaattcgcagattaataatactagagggcacaaagcctatgcgggctatgaccggttgttgtctgtggcgtaactccctgtgcattcgttgtagcatccagtacggttcttaaccgccgaattggctgcacggtcgtcgtcgtcgtaatctgctgatcaaaagcgcaaccaaaccggtcagcgttaatccgctgaaagttggtcaaccgctgggcggcgttctggctctgcaaggtatgtatcgttctatgccgctgctgcatggcgcacaaggttgttctgcgtttagtaaagcactgctgacccgtcattttcgcgaaccgattgcagttcaaacctctgctctgcaggaaatggacgtcatcttcgacgcggatcgtaatctggaagaagcgctggatcacatctggagcaaacatcatccggacgttattggcgttattagcaccgcactgaccgaagttgcaggcgtagattttcagagccgcgttaaagcattcaaacgcgaacgcgcactgaaagatagcctgctgtttagcgttagcctgccggattttcatggttctctggaaaccggctatagcagtaccgtcgaatctctgatggacgcagttctgggtctggcaggcggtaaaagtccgaaaaaacagcgtcgtacccaggttaatctgctgccggcaagttatctgaccgctggcgacgttatggagatcaaagacatcatcgcgagctttggcctggaagttatcaccctgccggatattagcaccagtctgtcaggtcatctgctgaccggttttagtccgctgacccgcggcggtaccccgctggattctgcttgtcagatgctggaaagcagctgtaccattgcgattggcgcaagtatggaacgtccggcacgtcgtctgacccacgctgcaggtattccgtatcacctgtttgcaggtctgtctggtctggcagcatctgacgcatttatccacttcctgcagaaaattagccgcgaaccggcaccggtacgttttcgttggcaacgcgaaaatctgctggatagcatgctggacgcgcatttttactatagcggcgcaagcgcggttgtagcactggaaccggatcatatgctgtctaccgcagcttggctggaagaaatgggggttgagctgaaacgtctgattaccccgtgtagtaccccggcactgcaaaaaaccgaacgcgaagtttggattggcgacctggacgacgcagaagaatctgcacaaggcgttgatctgtggatctctaatagtcacggtcgtaaaggcgcagctcgtgcaggtgcaagttttgttccggcaggtctgccggtatacgacgaactgggcgcacatacctctgtttctgttggttatcgcggtaccatggagtgggtcaacaaagtaggcaacgtcctgctggctgaacgcggtcgcggcggttaataatactagagtgccgagagggggaggggaggatgaaagttgcgtttgcaaccgaagacggcgttctggtaaacgcacattttggccagagcccgatgtttaccatcttcgaaatccgccactctggcgttcagtttctggaacatcgtcgtattgcgctgggttctgacgaaaacgaagcaggcaaaatcgcgagccgtattggtctgattgaagactgcgcgctgatctttctggttcaaattggcgcaagcgcagcagcacaagttaccaaacgcaccatcatgccggtcaaagttgcatttggcagcaccattgaagaacaggtacagcgtctgcagaatatgctgacccgtaatccgccgatgtggctggcaaaaattctgcacgcagaagaaggttctggtaaagcagaaagctaataatactagaggcacatggagggggtggacgaatggtgcaactgctggaagatagccgttacggtcgtcaactgaaactgctgggcgttgaaggtcagaatcgtctgaaacaagccaccgttatggttgcaggtattggcggtctgggcggcgctgcagcaatgtatctggctgctgctggtgttggtaaactgattctggctcacgaaggcgttatccatctgccggatatgaaccgtcaggttctgatggatagcggtcgtattggcgaagaacgtatggaaaccgcactgcaacatctgcatcgcattaacccggaaaccgaactggaaggtcacgcacatcgtattaccgaagaaagctctggtccgtgggttgaagcatctgatatcgtcatcgacgcacgttacgattttccggaacgttacgctctgaatcgtctgtgcgttcgtcacggtcgtccgatgattgaagcagcaatgtacgcgtacgaagtcagcctgatgaccattgatccggggaaaaccgcttgtctggagtgtctgtatccggaaggcggtcaaccgtgggaaccgctgggttttccggtactgggcgcaacctctggtctgattggttgtatggcagcactggaagccgttaaatggattaccgacgcaggtaacctgttcaccgatcgtatgtaccgcatgaacgtcctggatatgagcagctgtaccatcgcagtcaaacgtaatccgcgttgtccgtgttgtggtaccggcggcgataccgacgaaagcgtggcatatctgtaataatactagaggcggaacgggaggggatacagatgagccgtctgcatatttgcgataccaccctgcgtgacggcgaacaagcaccgggcgttgcgtttagcgcggaagagaaaaccgaaatcgcgattatgctggatagcgcaggcgttgaacaggcagaaattggcattccggcaatgggtaaaaccgagtgccgtagcattgcacgcattgcagctctgggtctgcaaatgaaactgatgacctggaaccgcgcagtttttaccgatatcgatgcaaccgaaagtaccggcgtaggttgggcacatattagcgttccggttagcaccgttcagatgaaatccaaactgggcatgaacccggaacaggttaccgaactgatccgcaaaagcgttgactacgcactgtgcaaaggtctgaccgttagcgttggttttgaagacgcaagtcgcgcagacgacctgtttctggaacaactggcaaaccagctgtatcgcgacggtattcgtcgttttcgctacgcagataccctgagcgttcatcatccggcagcaattgcagcacgtattgatcgtctggtttctcgcgttccgcaagacgttgaactggagatccactgccacaacgattacggtctggcactggcaaataccctggcagcactgcaagcaggcgcagtttgggcaagtaccaccgtttctggtctgggcgaacgcgcaggtaataccgcactggaagaggttgtcatgagctggcgcgatctgtatcagggtacctgttctgttcgtccggaactgctgaatccgctggcagcactggttagcaaagcgagcaatcgcattatcccggaaggtaaaccgattgtcggcgatatggtatttgcgcacgaaagcggcatccatattaacggtctgctgaaagaacgcgcagcatatcaagcactggatccgaccgaactgggtaccgatcatagctttgtcctgggcaaacactctggtcgttctgctgtccagtatatgctggaacaggaaggtattgaagcaggttctggcgagatcaaattcctgctggaacgtctgcgtctggttggcgaagatccgaaacgcgttatccattctgcagatctgcgtcgttggctgcaatattatccggcagaactgccgaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z