BBa_K1796015
type: ComponentDefinition
persistent identity: BBa K1796015
display id: BBa_K1796015
version: 1
was generated by: iGEM to SBOL conversion
description: complete line of nif cluster
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T09:54:28Z
mutable provenance: Paenibacillus sp.,promoted
owned by: james
owned by: myers
mutable description: whole line of nif cluster,contain Pnif,nifBHDKENXV,hesA
discontinued: false
dominant: false
group u list: _2221_
m user id: 23592
owner id: 23592
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: whole line of nif cluster
star: false
creator: Nannan Xie
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K1796014
component: BBa_K1796013
component: BBa_K1796012
component: BBa_K1796010
component: BBa_K1796011
component: BBa_K1796009
component: BBa_K1796008
component: BBa_K1796007
component: BBa_K1796006
component: BBa_K1796001
sequence annotation: BBa_K1796001
sequence annotation: BBa_K1796006
sequence annotation: BBa_K1796007
sequence annotation: BBa_K1796008
sequence annotation: BBa_K1796009
sequence annotation: BBa_K1796010
sequence annotation: BBa_K1796011
sequence annotation: BBa_K1796012
sequence annotation: BBa_K1796013
sequence annotation: BBa_K1796014
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1796015_sequence/1

BBa_B0015
type: Component
persistent identity: component2475751
display id: component2475751
version: 1
top level: BBa_K1796015
definition: BBa_B0015
access: public

BBa_K1796014
type: Component
persistent identity: component2475744
display id: component2475744
version: 1
top level: BBa_K1796015
definition: BBa_K1796014
access: public

BBa_K1796013
type: Component
persistent identity: component2475741
display id: component2475741
version: 1
top level: BBa_K1796015
definition: BBa_K1796013
access: public

BBa_K1796012
type: Component
persistent identity: component2475738
display id: component2475738
version: 1
top level: BBa_K1796015
definition: BBa_K1796012
access: public

BBa_K1796010
type: Component
persistent identity: component2475732
display id: component2475732
version: 1
top level: BBa_K1796015
definition: BBa_K1796010
access: public

BBa_K1796011
type: Component
persistent identity: component2475735
display id: component2475735
version: 1
top level: BBa_K1796015
definition: BBa_K1796011
access: public

BBa_K1796009
type: Component
persistent identity: component2475729
display id: component2475729
version: 1
top level: BBa_K1796015
definition: BBa_K1796009
access: public

BBa_K1796008
type: Component
persistent identity: component2475726
display id: component2475726
version: 1
top level: BBa_K1796015
definition: BBa_K1796008
access: public

BBa_K1796007
type: Component
persistent identity: component2475723
display id: component2475723
version: 1
top level: BBa_K1796015
definition: BBa_K1796007
access: public

BBa_K1796006
type: Component
persistent identity: component2475720
display id: component2475720
version: 1
top level: BBa_K1796015
definition: BBa_K1796006
access: public

BBa_K1796001
type: Component
persistent identity: component2475717
display id: component2475717
version: 1
top level: BBa_K1796015
definition: Pnif
access: public

BBa_K1796001
type: SequenceAnnotation
persistent identity: annotation2475717
display id: annotation2475717
version: 1
top level: BBa_K1796015
location: https://synbiohub.org/public/igem/BBa_K1796015/annotation2475717/range2475717/1
component: BBa_K1796001

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BBa_K1796006
type: SequenceAnnotation
persistent identity: annotation2475720
display id: annotation2475720
version: 1
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location: https://synbiohub.org/public/igem/BBa_K1796015/annotation2475720/range2475720/1
component: BBa_K1796006

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BBa_K1796007
type: SequenceAnnotation
persistent identity: annotation2475723
display id: annotation2475723
version: 1
top level: BBa_K1796015
location: https://synbiohub.org/public/igem/BBa_K1796015/annotation2475723/range2475723/1
component: BBa_K1796007

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BBa_K1796008
type: SequenceAnnotation
persistent identity: annotation2475726
display id: annotation2475726
version: 1
top level: BBa_K1796015
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component: BBa_K1796008

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BBa_K1796009
type: SequenceAnnotation
persistent identity: annotation2475729
display id: annotation2475729
version: 1
top level: BBa_K1796015
location: https://synbiohub.org/public/igem/BBa_K1796015/annotation2475729/range2475729/1
component: BBa_K1796009

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BBa_K1796010
type: SequenceAnnotation
persistent identity: annotation2475732
display id: annotation2475732
version: 1
top level: BBa_K1796015
location: https://synbiohub.org/public/igem/BBa_K1796015/annotation2475732/range2475732/1
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BBa_K1796011
type: SequenceAnnotation
persistent identity: annotation2475735
display id: annotation2475735
version: 1
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component: BBa_K1796011

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BBa_K1796012
type: SequenceAnnotation
persistent identity: annotation2475738
display id: annotation2475738
version: 1
top level: BBa_K1796015
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BBa_K1796013
type: SequenceAnnotation
persistent identity: annotation2475741
display id: annotation2475741
version: 1
top level: BBa_K1796015
location: https://synbiohub.org/public/igem/BBa_K1796015/annotation2475741/range2475741/1
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BBa_K1796014
type: SequenceAnnotation
persistent identity: annotation2475744
display id: annotation2475744
version: 1
top level: BBa_K1796015
location: https://synbiohub.org/public/igem/BBa_K1796015/annotation2475744/range2475744/1
component: BBa_K1796014

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display id: range2475744
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BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2475751
display id: annotation2475751
version: 1
top level: BBa_K1796015
location: https://synbiohub.org/public/igem/BBa_K1796015/annotation2475751/range2475751/1
component: BBa_B0015

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display id: range2475751
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BBa_K1796006
type: ComponentDefinition
persistent identity: BBa K1796006
display id: BBa_K1796006
version: 1
was generated by: iGEM to SBOL conversion
description: Dinitrogenase reductase(nifH)
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T07:21:59Z
mutable provenance: NifH(BBa_K1796006) is taken from genomic sequence of Paenibacillus sp. WLY78, we apply promotions on the sequences and synthesised it.
owned by: james
owned by: myers
mutable description: Dinitrogenase reductase, important in nitrogen fixation.
discontinued: false
dominant: false
group u list: _2221_
m user id: 12637
owner id: 23592
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We put it in a pET-28b plasmid to express it.
star: false
creator: Nannan Xie
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: unknown rbs
sequence annotation: nifH
sequence: https://synbiohub.org/public/igem/BBa_K1796006_sequence/1

unknown rbs
type: SequenceAnnotation
persistent identity: annotation2474986
display id: annotation2474986
version: 1
top level: BBa_K1796006
location: https://synbiohub.org/public/igem/BBa_K1796006/annotation2474986/range2474986/1
role: SO:0000139
role: feature/rbs

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type: Range
persistent identity: range2474986
display id: range2474986
version: 1
top level: BBa_K1796006
direction: direction/forward
start: 1
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nifH
type: SequenceAnnotation
persistent identity: annotation2456188
display id: annotation2456188
version: 1
top level: BBa_K1796006
location: https://synbiohub.org/public/igem/BBa_K1796006/annotation2456188/range2456188/1
role: SO:0000316
role: feature/cds

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type: Range
persistent identity: range2456188
display id: range2456188
version: 1
top level: BBa_K1796006
direction: direction/forward
start: 22
end: 891
orientation: inline

Pnif
type: ComponentDefinition
persistent identity: BBa K1796001
display id: BBa_K1796001
version: 1
was generated by: iGEM to SBOL conversion
description: promoter of Paenibacillus sp. WLY78 nif gene cluster
created: 2015-09-16T11:00:00Z
modified: 2015-09-18T02:35:42Z
mutable provenance: Pnif is from Paenibacillus sp. WLY78 genome,located upstream of nifB,the first gene of nitrogen fixation cluster.
owned by: james
owned by: myers
mutable description: The promoter of Paenibacillus sp. WLY78 nif gene cluster, We named it Pnif. Pnif is a σ70-dependent promoter located upstream of nifB. Pnif is in control of all the gene clusters of nitrogen fixation genome. Confirmation of nif promoter We standardized the nif promoter of Paenibacillus sp. WLY78, and linked it to the upstream of 3 reporter genes (RFP reporter: BBa_K1357010, YFP reporter: BBa_E0430;amilCP reporter: BBa_K1357009) to confirm the reliability of this promoter. The results are shown in Figure 2. A B C http://2015.igem.org/wiki/images/b/b2/Confirmation_of_Pnif_AB.png http://2015.igem.org/wiki/images/thumb/3/34/C-confirmation_of_Pnif.jpeg/622px-C-confirmation_of_Pnif.jpeg Fig.4. Confirmation of nif promoter of Paenibacillus sp. WLY78. (A) Pnif+RFP reporter; (B) Pnif+YFP reporter;(C)Pnif+amilCP reporter. The results confirmed the reliability of Pnif. The initiation of Pnif does not require inducement. Thus it is a effective constitutive promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2221_
m user id: 12637
owner id: 23592
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: effeciency of the promoter in E.coli for ordinary parts
star: false
creator: Nannan Xie
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Pnif
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K1796001_sequence/1

Pnif
type: SequenceAnnotation
persistent identity: annotation2473661
display id: annotation2473661
version: 1
top level: Pnif
location: https://synbiohub.org/public/igem/BBa_K1796001/annotation2473661/range2473661/1
role: feature/promoter
role: SO:0000167

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type: Range
persistent identity: range2473661
display id: range2473661
version: 1
top level: Pnif
direction: direction/forward
start: 1
end: 100
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2473637
display id: annotation2473637
version: 1
top level: Pnif
location: https://synbiohub.org/public/igem/BBa_K1796001/annotation2473637/range2473637/1
role: feature/operator
role: SO:0000057

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type: Range
persistent identity: range2473637
display id: range2473637
version: 1
top level: Pnif
direction: direction/forward
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end: 67
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2473660
display id: annotation2473660
version: 1
top level: Pnif
location: https://synbiohub.org/public/igem/BBa_K1796001/annotation2473660/range2473660/1
role: feature/operator
role: SO:0000057

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type: Range
persistent identity: range2473660
display id: range2473660
version: 1
top level: Pnif
direction: direction/forward
start: 85
end: 90
orientation: inline

BBa_K1796007
type: ComponentDefinition
persistent identity: BBa K1796007
display id: BBa_K1796007
version: 1
was generated by: iGEM to SBOL conversion
description: nifB from Paenibacillus sp. WLY78
created: 2015-09-16T11:00:00Z
modified: 2015-09-18T02:58:06Z
mutable provenance: NifB(BBa_K1796007) is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it.
owned by: james
owned by: myers
mutable description: essential for biosynthesis of the active-site nitrogenase cofactor and encodes a radical A-adenosylmethionine(SAM)-dependent enzyme that inserts the central carbon atom into the eight-Fe core of nifB cofactor(nifB-co).
discontinued: false
dominant: false
group u list: _2221_
m user id: 12637
owner id: 23592
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The part contains a sequence of 21bp possible RBS region
star: false
creator: Nannan Xie
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: unknown rbs
sequence annotation: nifB coding seqquence
sequence: https://synbiohub.org/public/igem/BBa_K1796007_sequence/1

unknown rbs
type: SequenceAnnotation
persistent identity: annotation2475008
display id: annotation2475008
version: 1
top level: BBa_K1796007
location: https://synbiohub.org/public/igem/BBa_K1796007/annotation2475008/range2475008/1
role: SO:0000139
role: feature/rbs

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type: Range
persistent identity: range2475008
display id: range2475008
version: 1
top level: BBa_K1796007
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nifB coding seqquence
type: SequenceAnnotation
persistent identity: annotation2473679
display id: annotation2473679
version: 1
top level: BBa_K1796007
location: https://synbiohub.org/public/igem/BBa_K1796007/annotation2473679/range2473679/1
role: SO:0000316
role: feature/cds

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type: Range
persistent identity: range2473679
display id: range2473679
version: 1
top level: BBa_K1796007
direction: direction/forward
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end: 1524
orientation: inline

BBa_K1796008
type: ComponentDefinition
persistent identity: BBa K1796008
display id: BBa_K1796008
version: 1
was generated by: iGEM to SBOL conversion
description: nifD promoted from Paenibacillus sp. WLY78
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T08:19:32Z
mutable provenance: NifD(BBa_K1796008) is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it.
owned by: james
owned by: myers
mutable description: Function: αsubunits of dinitrogease which also called FeMo protein. NifD encode a metallocluster: FeMo-co, a [Mo-7Fe-9S-C-homocitrate] cluster which serves as the active site of substrate binding and reduction. We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But we were not informed of the error in promotion.After several times failed expriments, we found the problem.We tackled them over the matter, mistakes were corrected, but the correct genes can't arrive on time,it can only arrive after the deadline. Sequence submit is promoted by the synthesis company, containing a PstI in the gene. The sequence we promoted: CAGGAGGGAGGAATAGGCCAAATGTCTTCTATCGTTGACAAAGGTAAACAGATCGTTGAAGAAATCCTGGAAGTTTACCCGAAAAAAGCTAAAAAAGACCGTACCAAACACTTCGAAATCGCTGACGAAGAACTGGTTAACTGCGGTACCTGCTCTATCAAATCTAACATGAAATCTCGTCCGGGTGTTATGACCGCTCGTGGTTGCGCTTACGCTGGTTCTAAAGGTGTTGTTTGGGGTCCGATCAAAGACATGGTTCACATCTCTCACGGTCCGATCGGTTGCGGTCAGTACTCTTGGGGTACCCGTCGTAACTACGCTAACGGTATCCTGGGTATCGACAACTTCACCGCTATGCAGATCACCTCTAACTTCCAGGAAAAAGACATCGTTTTCGGTGGTGACAAAAAACTGGAAGTTATCTGCCGTGAAATCAAAGAAATGTTCCCGCTGGCTAAAGGTATCTCTGTTCAGTCTGAATGCCCGGTTGGTCTGATCGGTGACGACATCGGTGCTGTTGCTAAAAAAATGACCGAAGAACTGGGTATCCCGGTTATCCCGGTTCGTTGCGAAGGTTTCCGTGGTGTTTCTCAGTCTCTGGGTCACCACATCGCTAACGACGCTATCCGTGACTTCCTGATGGGTCGTCGTGAACTGAAAGAATGCGGTCCGTACGACGTTTCTATCATCGGTGACTACAACATCGGTGGTGACGCTTGGGCTTCTCGTATCCTGCTGGAAGAAATGGGTCTGCGTGTTATCGCTCAGTGGTCTGGTGACGGTACCATCAACGAACTGGGTATCGCTCACAAATCTAAACTGAACCTGATCCACTGCCACCGTTCTATGAACTACATGTGCACCACCATGGAACAGGAATACGGTATCCCGTGGATGGAATACAACTTCTTCGGTCCGACCAAAACCATGGAATCTCTGCGTGCTATCGCTGCTCGTTTCGACGAAACCATCCAGGAAAAATGCGAACAGGTTATCGCTCAGTACATGCCGCAGATGGAAGCTGTTATCCGTAAATACCGTCCGCGTCTGGAAGGTAAAAAAGTTATGCTGCTGATCGGTGGTCTGCGTGCTCGTCACACCATCGGTGCTTACGAAGACCTGGGTATGGAAATCGTTGCTACCGGTTACGAATTTGCTCACAAAGA…
discontinued: false
dominant: false
group u list: _2221_
m user id: 23592
owner id: 23592
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But the part can be assembled by gibson assembly,that is what we did.
star: false
creator: Nannan Xie
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: unknown rbs
sequence annotation: nifD coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K1796008_sequence/1

unknown rbs
type: SequenceAnnotation
persistent identity: annotation2475048
display id: annotation2475048
version: 1
top level: BBa_K1796008
location: https://synbiohub.org/public/igem/BBa_K1796008/annotation2475048/range2475048/1
role: SO:0000139
role: feature/rbs

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type: Range
persistent identity: range2475048
display id: range2475048
version: 1
top level: BBa_K1796008
direction: direction/forward
start: 1
end: 21
orientation: inline

nifD coding sequence
type: SequenceAnnotation
persistent identity: annotation2466009
display id: annotation2466009
version: 1
top level: BBa_K1796008
location: https://synbiohub.org/public/igem/BBa_K1796008/annotation2466009/range2466009/1
role: feature/cds
role: SO:0000316

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type: Range
persistent identity: range2466009
display id: range2466009
version: 1
top level: BBa_K1796008
direction: direction/forward
start: 22
end: 1473
orientation: inline

BBa_K1796009
type: ComponentDefinition
persistent identity: BBa K1796009
display id: BBa_K1796009
version: 1
was generated by: iGEM to SBOL conversion
description: nifK promoted from Paenibacillus sp. WLY78
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T08:42:28Z
mutable provenance: NifK(BBa_K1796009) is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it.
owned by: james
owned by: myers
mutable description: Function: β subunits of dinitrogease which also called FeMo protein.NifK encode a metallocluster: the P-cluster, a [8Fe-7S] cluster which shuttles electrons to FeMo-co. We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis.
discontinued: false
dominant: false
group u list: _2221_
m user id: 23592
owner id: 23592
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis,the part can be assembled by gibson assembly,that is what we did.
star: false
creator: Nannan Xie
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: uknown rbs
sequence annotation: nifK coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K1796009_sequence/1

uknown rbs
type: SequenceAnnotation
persistent identity: annotation2475079
display id: annotation2475079
version: 1
top level: BBa_K1796009
location: https://synbiohub.org/public/igem/BBa_K1796009/annotation2475079/range2475079/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1796009/annotation2475079/range2475079/1
type: Range
persistent identity: range2475079
display id: range2475079
version: 1
top level: BBa_K1796009
direction: direction/forward
start: 1
end: 21
orientation: inline

nifK coding sequence
type: SequenceAnnotation
persistent identity: annotation2473682
display id: annotation2473682
version: 1
top level: BBa_K1796009
location: https://synbiohub.org/public/igem/BBa_K1796009/annotation2473682/range2473682/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1796009/annotation2473682/range2473682/1
type: Range
persistent identity: range2473682
display id: range2473682
version: 1
top level: BBa_K1796009
direction: direction/forward
start: 22
end: 1554
orientation: inline

BBa_K1796010
type: ComponentDefinition
persistent identity: BBa K1796010
display id: BBa_K1796010
version: 1
was generated by: iGEM to SBOL conversion
description: nifE promoted from Paenibacillus sp. WLY78
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T08:39:40Z
mutable provenance: NifE(BBa_K1796010) is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it.
owned by: james
owned by: myers
mutable description: Function: scaffold
discontinued: false
dominant: false
group u list: _2221_
m user id: 23592
owner id: 23592
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselvesThen we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But the part can be assembled by gibson assembly,that is what we did.
star: false
creator: Nannan Xie
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: unknown rbs
sequence annotation: nifE
sequence: https://synbiohub.org/public/igem/BBa_K1796010_sequence/1

unknown rbs
type: SequenceAnnotation
persistent identity: annotation2475102
display id: annotation2475102
version: 1
top level: BBa_K1796010
location: https://synbiohub.org/public/igem/BBa_K1796010/annotation2475102/range2475102/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1796010/annotation2475102/range2475102/1
type: Range
persistent identity: range2475102
display id: range2475102
version: 1
top level: BBa_K1796010
direction: direction/forward
start: 1
end: 21
orientation: inline

nifE
type: SequenceAnnotation
persistent identity: annotation2473683
display id: annotation2473683
version: 1
top level: BBa_K1796010
location: https://synbiohub.org/public/igem/BBa_K1796010/annotation2473683/range2473683/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1796010/annotation2473683/range2473683/1
type: Range
persistent identity: range2473683
display id: range2473683
version: 1
top level: BBa_K1796010
direction: direction/forward
start: 22
end: 1386
orientation: inline

BBa_K1796011
type: ComponentDefinition
persistent identity: BBa K1796011
display id: BBa_K1796011
version: 1
was generated by: iGEM to SBOL conversion
description: nifN promoted from Paenibacillus sp. WLY78
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T08:46:04Z
mutable provenance: NifN is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it.
owned by: james
owned by: myers
mutable description: Function: scaffold gene of nitrogen fixation system
discontinued: false
dominant: false
group u list: _2221_
m user id: 23592
owner id: 23592
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut sites were involved(three PstI) after they promoted it again. But the part can be assembled by gibson assembly,that is what we did.
star: false
creator: Nannan Xie
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: unknown rbs
sequence annotation: nifN coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K1796011_sequence/1

unknown rbs
type: SequenceAnnotation
persistent identity: annotation2475143
display id: annotation2475143
version: 1
top level: BBa_K1796011
location: https://synbiohub.org/public/igem/BBa_K1796011/annotation2475143/range2475143/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1796011/annotation2475143/range2475143/1
type: Range
persistent identity: range2475143
display id: range2475143
version: 1
top level: BBa_K1796011
direction: direction/forward
start: 1
end: 21
orientation: inline

nifN coding sequence
type: SequenceAnnotation
persistent identity: annotation2473704
display id: annotation2473704
version: 1
top level: BBa_K1796011
location: https://synbiohub.org/public/igem/BBa_K1796011/annotation2473704/range2473704/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1796011/annotation2473704/range2473704/1
type: Range
persistent identity: range2473704
display id: range2473704
version: 1
top level: BBa_K1796011
direction: direction/forward
start: 22
end: 1434
orientation: inline

BBa_K1796012
type: ComponentDefinition
persistent identity: BBa K1796012
display id: BBa_K1796012
version: 1
was generated by: iGEM to SBOL conversion
description: nifX promoted from Paenibacillus sp. WLY78
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T08:52:34Z
mutable provenance: NifX(BBa_K1796012) is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it.
owned by: james
owned by: myers
mutable description: Function: encode carrier proteins involved in the delivery of FeMoco to the MoFe protein , participate in FeMoco assembly in a chaperone-like function.
discontinued: false
dominant: false
group u list: _2221_
m user id: 23592
owner id: 23592
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site（one PstI） was involved after they promoted it again. But the part can be assembled by gibson assembly,that is what we did.
star: false
creator: Nannan Xie
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: unknown rbs
sequence annotation: nifX coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K1796012_sequence/1

unknown rbs
type: SequenceAnnotation
persistent identity: annotation2475144
display id: annotation2475144
version: 1
top level: BBa_K1796012
location: https://synbiohub.org/public/igem/BBa_K1796012/annotation2475144/range2475144/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1796012/annotation2475144/range2475144/1
type: Range
persistent identity: range2475144
display id: range2475144
version: 1
top level: BBa_K1796012
direction: direction/forward
start: 1
end: 21
orientation: inline

nifX coding sequence
type: SequenceAnnotation
persistent identity: annotation2473705
display id: annotation2473705
version: 1
top level: BBa_K1796012
location: https://synbiohub.org/public/igem/BBa_K1796012/annotation2473705/range2473705/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1796012/annotation2473705/range2473705/1
type: Range
persistent identity: range2473705
display id: range2473705
version: 1
top level: BBa_K1796012
direction: direction/forward
start: 22
end: 414
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1796013
type: ComponentDefinition
persistent identity: BBa K1796013
display id: BBa_K1796013
version: 1
was generated by: iGEM to SBOL conversion
description: hesA promoted from Paenibacillus sp. WLY78
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T08:57:35Z
mutable provenance: It is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it.
owned by: james
owned by: myers
mutable description: Function has not so far been determined , but Deletion analysis indicates that in addition to the core nif genes, hesA plays an important role in nitrogen fixation and is responsive to the availability of molybdenum. HesA a member of the ThiF-MoeB-HesA family and contains an N-terminal nucleotide binding domain and a C-terminal MoeZ/MoeB-like domain. The ThiF-MoeB-HesA family engage in an ATP-dependent process that activates the C-terminus of partner ubiquitin-like proteins by forming an acyl adenylate complex that facilitates sulfur transfer. First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But we were not informed of the error in promotion.After several times failed expriments, we found the problem.We tackled them over the matter, mistakes were corrected, but the correct genes can't arrive on time,it can only arrive after the deadline. Sequence submit is promoted by the synthesis company, containing a PstI in the gene
discontinued: false
dominant: false
group u list: _2221_
m user id: 23592
owner id: 23592
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But the part can be assembled by gibson assembly,that is what we did.
star: false
creator: Nannan Xie
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: unknown rbs
sequence annotation: hesA coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K1796013_sequence/1

unknown rbs
type: SequenceAnnotation
persistent identity: annotation2475145
display id: annotation2475145
version: 1
top level: BBa_K1796013
location: https://synbiohub.org/public/igem/BBa_K1796013/annotation2475145/range2475145/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1796013/annotation2475145/range2475145/1
type: Range
persistent identity: range2475145
display id: range2475145
version: 1
top level: BBa_K1796013
direction: direction/forward
start: 1
end: 21
orientation: inline

hesA coding sequence
type: SequenceAnnotation
persistent identity: annotation2473742
display id: annotation2473742
version: 1
top level: BBa_K1796013
location: https://synbiohub.org/public/igem/BBa_K1796013/annotation2473742/range2473742/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1796013/annotation2473742/range2473742/1
type: Range
persistent identity: range2473742
display id: range2473742
version: 1
top level: BBa_K1796013
direction: direction/forward
start: 22
end: 789
orientation: inline

BBa_K1796014
type: ComponentDefinition
persistent identity: BBa K1796014
display id: BBa_K1796014
version: 1
was generated by: iGEM to SBOL conversion
description: nifV promoted from Paenibacillus sp. WLY78
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T09:03:19Z
mutable provenance: It is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it.
owned by: james
owned by: myers
mutable description: homocitrate synthase，provides the organic moiety in all three nitrogenase cofactors. First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But we were not informed of the error in promotion.After several times failed expriments, we found the problem.We tackled them over the matter, mistakes were corrected, but the correct genes can't arrive on time,it can only arrive after the deadline. Sequence submit is promoted by the synthesis company, containing a PstI in the gene.
discontinued: false
dominant: false
group u list: _2221_
m user id: 23592
owner id: 23592
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves.Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But the part can be assembled by gibson assembly,that is what we did.
star: false
creator: Nannan Xie
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: unkown rbs
sequence annotation: nifV
sequence: https://synbiohub.org/public/igem/BBa_K1796014_sequence/1

unkown rbs
type: SequenceAnnotation
persistent identity: annotation2475151
display id: annotation2475151
version: 1
top level: BBa_K1796014
location: https://synbiohub.org/public/igem/BBa_K1796014/annotation2475151/range2475151/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1796014/annotation2475151/range2475151/1
type: Range
persistent identity: range2475151
display id: range2475151
version: 1
top level: BBa_K1796014
direction: direction/forward
start: 1
end: 21
orientation: inline

nifV
type: SequenceAnnotation
persistent identity: annotation2473746
display id: annotation2473746
version: 1
top level: BBa_K1796014
location: https://synbiohub.org/public/igem/BBa_K1796014/annotation2473746/range2473746/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1796014/annotation2473746/range2473746/1
type: Range
persistent identity: range2473746
display id: range2473746
version: 1
top level: BBa_K1796014
direction: direction/forward
start: 22
end: 1161
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1796015_sequence/1
type: Sequence
persistent identity: BBa K1796015 sequence
display id: BBa_K1796015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtaaaatttgacacatatgtgaattgaggataaatgtcagggatttcatggagaagtgaattgactgtatttgtccctgtctctaagatgtaattatattactagagcattttggaggctgagaaaatatgcgtcagatcgctttctacggtaaaggtggtatcggtaaatctaccacctctcagaacaccctggctcagctggctaccaaattcaaacagaaaatcatgatcgttggttgcgacccgaaagctgactctacccgtctgatcctgaacaccaaagctcagcagaccgttctgcacctggctgctgaacgtggtaccgttgaagacctggaactggaagacgttgttcagaaaggtttcggtgacatcctgaacgttgaatgcggtggtccggaaccgggtgttggttgcgctggtcgtggtatcatcaccgctatcaacttcctggaagaagaaggtgcttacgaaggtctggacttcgtttcttacgacgttctgggtgacgttgtttgcggtggtttcgctatgccgatccgtgaaaaaaaagctcaggaaatctacatcgtttgctctggtgaaatgatggctatgtacgctgctaacaacatcgctcgtggtatcctgaaatacgctaactctggtggtgttcgtctgggtggtctgatctgcaactctcgtaacaccgacctggaagctgaactgatcaccgaactggctcgtcgtctgaacacccagatgatccacttcctgccgcgtgacaacgttgttcagcacgctgaactgcgtcgtatgaccgttacccagtacaacccggaacacaaacaggctgctgaatacgaagaactggctggtaaaatcctgaacaacgacatgctgaccgttccgaccccgatctctatggaagacctggaagacctgctgatggaatttggtatcatcgaagacgaagaaaccgctatcaacaaagctgaagcttctggtcagtaataatactagagagagaacgtagagaggagggaatggatagcttagcggatctgagcgaaaccccgttagcattagaaaccctgcgtcgtcatccgtgttataacgaagaagcgcatcgctactttgcgcgtattcatttaccggttgcaccggcttgcaacattcagtgccactactgcaaccgcaaattcgactgcgttaacgaaagccgtccgggcgttgtttctgaattattaaccccggaacaggcagcaagtaaaacctacggcgttgctgcccaactgatgcagttaagcgttgttggtattgcaggtccgggcgatccgttagcaaacgcggaagcgacctttgatacctttcgtcgcgttcgcgaaaccgttaaagacgttatcttttgcctgagcaccaacggtctgaccttaattcgccacatcgatcgcatcgttgaactgggtattagccacgtcaccatcaccatcaacgcagttgatccggttgttggtagtcgcatttacggttgggtttacgacgaaggcaaacgttatgcaggcgaagaagcagcacgtttactgattgaccgtcagctggctggtctgaaaatgttagcatctcgcggcgttctgtgcaaagttaacagcgttctgatcccggaagttaacgacgcacatttaccggaagttgcacgcgtagttaaagaacacggcgcagttctgcacaacatcatgccgctgattattgcaccgggttctcgttacgaacaagaaggtatgcgcgcaccgcgtccgcgtttagttcgtcagttacaggaacagtgcgcagaagctggcgctgttattatgcgtcattgtcgtcagtgtcgcgcagacgcaattggtttactgggcgaagatcgtaaccaggattttacctgggaaaatattgcggcagctccgccgatggacgaagaagcacgcgcacaatttcagaaagagctggacgaaaaagtccgcgttcgtatggaacgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1796006_sequence/1
type: Sequence
persistent identity: BBa K1796006 sequence
display id: BBa_K1796006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cattttggaggctgagaaaatatgcgtcagatcgctttctacggtaaaggtggtatcggtaaatctaccacctctcagaacaccctggctcagctggctaccaaattcaaacagaaaatcatgatcgttggttgcgacccgaaagctgactctacccgtctgatcctgaacaccaaagctcagcagaccgttctgcacctggctgctgaacgtggtaccgttgaagacctggaactggaagacgttgttcagaaaggtttcggtgacatcctgaacgttgaatgcggtggtccggaaccgggtgttggttgcgctggtcgtggtatcatcaccgctatcaacttcctggaagaagaaggtgcttacgaaggtctggacttcgtttcttacgacgttctgggtgacgttgtttgcggtggtttcgctatgccgatccgtgaaaaaaaagctcaggaaatctacatcgtttgctctggtgaaatgatggctatgtacgctgctaacaacatcgctcgtggtatcctgaaatacgctaactctggtggtgttcgtctgggtggtctgatctgcaactctcgtaacaccgacctggaagctgaactgatcaccgaactggctcgtcgtctgaacacccagatgatccacttcctgccgcgtgacaacgttgttcagcacgctgaactgcgtcgtatgaccgttacccagtacaacccggaacacaaacaggctgctgaatacgaagaactggctggtaaaatcctgaacaacgacatgctgaccgttccgaccccgatctctatggaagacctggaagacctgctgatggaatttggtatcatcgaagacgaagaaaccgctatcaacaaagctgaagcttctggtcagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1796001_sequence/1
type: Sequence
persistent identity: BBa K1796001 sequence
display id: BBa_K1796001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtaaaatttgacacatatgtgaattgaggataaatgtcagggatttcatggagaagtgaattgactgtatttgtccctgtctctaagatgtaattatat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1796007_sequence/1
type: Sequence
persistent identity: BBa K1796007 sequence
display id: BBa_K1796007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaacgtagagaggagggaatggatagcttagcggatctgagcgaaaccccgttagcattagaaaccctgcgtcgtcatccgtgttataacgaagaagcgcatcgctactttgcgcgtattcatttaccggttgcaccggcttgcaacattcagtgccactactgcaaccgcaaattcgactgcgttaacgaaagccgtccgggcgttgtttctgaattattaaccccggaacaggcagcaagtaaaacctacggcgttgctgcccaactgatgcagttaagcgttgttggtattgcaggtccgggcgatccgttagcaaacgcggaagcgacctttgatacctttcgtcgcgttcgcgaaaccgttaaagacgttatcttttgcctgagcaccaacggtctgaccttaattcgccacatcgatcgcatcgttgaactgggtattagccacgtcaccatcaccatcaacgcagttgatccggttgttggtagtcgcatttacggttgggtttacgacgaaggcaaacgttatgcaggcgaagaagcagcacgtttactgattgaccgtcagctggctggtctgaaaatgttagcatctcgcggcgttctgtgcaaagttaacagcgttctgatcccggaagttaacgacgcacatttaccggaagttgcacgcgtagttaaagaacacggcgcagttctgcacaacatcatgccgctgattattgcaccgggttctcgttacgaacaagaaggtatgcgcgcaccgcgtccgcgtttagttcgtcagttacaggaacagtgcgcagaagctggcgctgttattatgcgtcattgtcgtcagtgtcgcgcagacgcaattggtttactgggcgaagatcgtaaccaggattttacctgggaaaatattgcggcagctccgccgatggacgaagaagcacgcgcacaatttcagaaagagctggacgaaaaagtccgcgttcgtatggaacgtaaagaaggccagagccatcataaacaaccgagtaccggcgcaggttgtagttgtccgttaagcggcgataaaccggaagcaagcttcaccagcaaaccggttctgattgcagttgcaagtcgcggcggcggtaaagttaatcagcatttcggccgcgccaaagaattcatgatctacgaaagcgacggcaccattgtcaacttcatcggcattcgcaaagtccagagctactgtcacggtaaagcggattgtaacggcgacaaagcggagaccatcaaagagatcctgagcatggttcacgattgcgcactgttactgagctctggtattggcgaagcaccgaaagaagccttacaggaagctggcgttttaccgattgtttgcggcggcgatattgaagaaagcgttctggagtacgtcaaattcctgcgctacatgtatccggttcagaccggtaaaggcagcaaacgtaacaaaggcgtcaaaggtaaccacagcgatctgccgattgaacattttgggggttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1796008_sequence/1
type: Sequence
persistent identity: BBa K1796008 sequence
display id: BBa_K1796008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caggagggaggaataggccaaatgagcagcatcgtcgacaaaggcaaacagatcgtggaagaaatcctggaagtctacccgaaaaaagcgaaaaaagaccgcaccaaacacttcgagatcgcggacgaagaactggttaactgcggtacctgcagcatcaaaagcaacatgaaaagtcgtccgggcgttatgacggcacgtggttgtgcatacgcaggttcaaaaggcgttgtttggggtccgatcaaagacatggtccatattagccacggtccgattggttgcggtcaatattcttggggtacccgtcgtaactacgcaaacggtatcctgggcatcgataacttcaccgcaatgcagatcaccagcaacttccaggaaaaagacatcgtcttcggcggcgacaaaaaactggaagtcatctgccgcgaaatcaaagagatgtttccgctggcgaaaggcattagcgttcaatctgagtgtccggttggtctgattggcgacgatattggcgcggtcgccaaaaagatgaccgaagaactgggcattccggttattccggttcgttgcgaaggttttcgcggggttagtcagagcctgggtcatcatattgcaaacgacgcaattcgcgattttctgatgggccgtcgcgaactgaaagagtgcggtccgtacgacgttagcatcattggcgattacaacattggcggcgatgcttgggcaagtcgtattctgctggaagaaatgggtctgcgcgttattgcacagtggtctggcgacggtaccattaacgaactgggtatcgcgcacaaaagcaaactgaacctgatccactgccatcgttccatgaactacatgtgcaccaccatggaacaggaatacggtatcccgtggatggaatacaacttcttcggtccgaccaaaaccatggaaagcctgcgcgcaattgcagcgcgttttgacgaaaccatccaggagaaatgcgagcaggttattgcccagtatatgccgcagatggaggcggtcattcgtaaatatcgcccgcgtctggagggcaaaaaagttatgctgctgattggcggtctgcgcgcacgtcataccattggcgcatacgaagatctgggcatggaaattgttgcgaccggttacgaatttgcgcacaaagacgactacgagaaaaccttcccggacgtcaaagagggcaccattctgtacgacgatccgaccgcatacgaactggaagaactggcacagcgtctgaacattgatctgatgggcgcaggcgtcaaagagaaatacgtctaccacaaaatgggcattccgttccgtcagatgcatagctgggattatagcggtccgtatcacggtttcgacggctttaaaatcttcgcccgcgatatggatatgaccatcaacagcccggtttggagtctgctgccgtctcgtcaaaccgcagaagttccggtttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1796009_sequence/1
type: Sequence
persistent identity: BBa K1796009 sequence
display id: BBa_K1796009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcagactgcggaggtgccggtatgagcgaacgtccgaacatcgttgatcacaaccagctgttccgccaggataaatacgtccgtcaacgcgaagagaaacgcgcttttgaagcaccgtgtagtccggaagaagttaccgataccctggagtacaccaaaaccaaagagtacaaagacaaaaactttgcgcgcaccgcggttgttgttaatccggcaaaagcctgtcaaccgttaggcgcagttatggcagcactgggttttgagaaaaccctgccgtttattcacggctctcagggttgtaccgcatattttcgcagccatctggcacgtcatttcaaagaaccggttccggctgtttctaccagtatgacggaagacgcagctgtttttggcggtatgcgtaacctgatcgacggcattgaaaactgcattgcgctgtaccagccggaaatgattgcagtttgcaccacctgcatggcagaagttattggcgacgatctgagcgcatttctggcaaacgcacgtcaagaaggcgttttaccggaagatatgccggttccgtttgcaaacaccccgagttttagcggtagccatatcaccggctacgacgcaatgttacgtagcgtcctggaaaccctgtacaacaaaagcggtcgtaccgcacaaccgggtcacgaattaaaactgaacgtcctgctgggctttgacggttataccggtaacttcgcggaaatgcgtcgtatgctgggtatgtttggggcaacctataccatcctgggcgatcacagcagcaactttgattctggcgcgaccggcgaatatagctattattacggtggtaccccgttagaagacgttccgaaagcagcagacgcagcaggtaccttagcaattcagcagtacagcctgcgtaaaaccctgggctatatgaaacagacctggggtcagcaagttagcagcatttctaccccgctgggtattcgcgcaaccgatcgcttactggaagaaattagccgtttaagcggtcgcgaaattccggaagcactgaaacaggaacgcgcacgtattgttgacgctatgatggatagccacgcatatctgcacggtaaacgcgttgcaatggcaggcgatccggatatgttaatcggtctgatcggcttctgcttagaactgggcatggaaccggtacatatcgtttgcagcaacggcgatcgcaaattcgagaaagaagcggaactgctgctgaaatcctctccgtatggcgcagaagcaaccgttcattctggtcaggatctgtggcatatgcgtagcctgctgtttcaagatccggttgatctggctattggtagcagccatctgaaatttgcggcgaaagaggcggaaattccgttactgcgcgttggttttccgatctttgatcgccatcacctgcaccgttatccgattattggctatcagggcgcactgaatctgctgacccagtttgtcaacaccatcctggacgtcatggaagaacaagcaccggatcatagctttgacttagttcgttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1796010_sequence/1
type: Sequence
persistent identity: BBa K1796010 sequence
display id: BBa_K1796010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caggagggaggaataggccaaatggaaccggcagttagtaacggtcgtctggaagtcagctgcggcaacaaaatcccgaaatctaccccgtgtccgcgtccggttccgggtgaagcatctggcggttgttcttttgacggtgcacagatcaccctgattccgattgcagatgcagcacatctggttcacggtccgattgcttgtctgggtaacagttgggaaagtcgcggtagtctgagttctggtccggaactgtctgcatacggttttaccaccgatctgggcgaacaagacatcatcttcggcagcgaacagaaactgcacgaaagcatccgctatattgtcagccgttttgcaccgccggccgtttttgtttataccacctgtgttaccgcactgaccggcgaagatattgaaggcgtctgcaaagcggaaagcgaacgtctgggtaccccgattattccggttaacagtccgggctttgtcggtagcaaaaatctgggtacccgtctggcaggcgacgttctgtttcagcatatcatcggtagtaccgaaccggaacagaccaccagtcacgatatcaacctgatcggcgaatacaatatcgccggcgaaatgtggcatatcgaacgtctgatgcaacaggcaggtatgagcattctgagccgtattaccggcgatggtcgttttcgcgaagttggttgggcacatcgtgccaaagcgaacatggtcgtttgtagtcgcgcactgctgggtctggctgttcaaatggaacgcaaatacggcatcccgtacttcgaaggtagcttttacggcgcaaaagaaaccagctattccctgcgccaaatggcatatctgaccggcgatcgcgacgttgaacgccgcgttgataaactggctgctcgcgaagaaatgcgtctgagtctggaactggaaccgtatcgcaaacagctgaaaggcaaacgcgcagttctgtataccggcggcgttaaaagctggtctgttattaccgcgctgcaggaactgggtattaaagtcgttggcgttggtaccaataaatctaccgcggaagatgttagccgtattgcggatcgtattggcgacgatgcagaatatattccggaaggcggtgcacgtcaaattctgaaaaccgttcgcagccgcaaagcagatatggttattgctggcggtcgcaacatgtacatggcactgaaagagcagatcccgtttgtcgacatcaaccaggaacgtcataaagcgtacgctggttacgacggtctgctgagtctggcaaaacagctggttcataccctgcaacatccggtttgggaactgaccgcaaaactggcaccgtgggaagaagaaaccgaattcgcagattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1796011_sequence/1
type: Sequence
persistent identity: BBa K1796011 sequence
display id: BBa_K1796011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcacaaagcctatgcgggctatgaccggttgttgtctgtggcgtaactccctgtgcattcgttgtagcatccagtacggttcttaaccgccgaattggctgcacggtcgtcgtcgtcgtaatctgctgatcaaaagcgcaaccaaaccggtcagcgttaatccgctgaaagttggtcaaccgctgggcggcgttctggctctgcaaggtatgtatcgttctatgccgctgctgcatggcgcacaaggttgttctgcgtttagtaaagcactgctgacccgtcattttcgcgaaccgattgcagttcaaacctctgctctgcaggaaatggacgtcatcttcgacgcggatcgtaatctggaagaagcgctggatcacatctggagcaaacatcatccggacgttattggcgttattagcaccgcactgaccgaagttgcaggcgtagattttcagagccgcgttaaagcattcaaacgcgaacgcgcactgaaagatagcctgctgtttagcgttagcctgccggattttcatggttctctggaaaccggctatagcagtaccgtcgaatctctgatggacgcagttctgggtctggcaggcggtaaaagtccgaaaaaacagcgtcgtacccaggttaatctgctgccggcaagttatctgaccgctggcgacgttatggagatcaaagacatcatcgcgagctttggcctggaagttatcaccctgccggatattagcaccagtctgtcaggtcatctgctgaccggttttagtccgctgacccgcggcggtaccccgctggattctgcttgtcagatgctggaaagcagctgtaccattgcgattggcgcaagtatggaacgtccggcacgtcgtctgacccacgctgcaggtattccgtatcacctgtttgcaggtctgtctggtctggcagcatctgacgcatttatccacttcctgcagaaaattagccgcgaaccggcaccggtacgttttcgttggcaacgcgaaaatctgctggatagcatgctggacgcgcatttttactatagcggcgcaagcgcggttgtagcactggaaccggatcatatgctgtctaccgcagcttggctggaagaaatgggggttgagctgaaacgtctgattaccccgtgtagtaccccggcactgcaaaaaaccgaacgcgaagtttggattggcgacctggacgacgcagaagaatctgcacaaggcgttgatctgtggatctctaatagtcacggtcgtaaaggcgcagctcgtgcaggtgcaagttttgttccggcaggtctgccggtatacgacgaactgggcgcacatacctctgtttctgttggttatcgcggtaccatggagtgggtcaacaaagtaggcaacgtcctgctggctgaacgcggtcgcggcggttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1796012_sequence/1
type: Sequence
persistent identity: BBa K1796012 sequence
display id: BBa_K1796012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgccgagagggggaggggaggatgaaagttgcgtttgcaaccgaagacggcgttctggtaaacgcacattttggccagagcccgatgtttaccatcttcgaaatccgccactctggcgttcagtttctggaacatcgtcgtattgcgctgggttctgacgaaaacgaagcaggcaaaatcgcgagccgtattggtctgattgaagactgcgcgctgatctttctggttcaaattggcgcaagcgcagcagcacaagttaccaaacgcaccatcatgccggtcaaagttgcatttggcagcaccattgaagaacaggtacagcgtctgcagaatatgctgacccgtaatccgccgatgtggctggcaaaaattctgcacgcagaagaaggttctggtaaagcagaaagctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1796013_sequence/1
type: Sequence
persistent identity: BBa K1796013 sequence
display id: BBa_K1796013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcacatggagggggtggacgaatggtgcaactgctggaagatagccgttacggtcgtcaactgaaactgctgggcgttgaaggtcagaatcgtctgaaacaagccaccgttatggttgcaggtattggcggtctgggcggcgctgcagcaatgtatctggctgctgctggtgttggtaaactgattctggctcacgaaggcgttatccatctgccggatatgaaccgtcaggttctgatggatagcggtcgtattggcgaagaacgtatggaaaccgcactgcaacatctgcatcgcattaacccggaaaccgaactggaaggtcacgcacatcgtattaccgaagaaagctctggtccgtgggttgaagcatctgatatcgtcatcgacgcacgttacgattttccggaacgttacgctctgaatcgtctgtgcgttcgtcacggtcgtccgatgattgaagcagcaatgtacgcgtacgaagtcagcctgatgaccattgatccggggaaaaccgcttgtctggagtgtctgtatccggaaggcggtcaaccgtgggaaccgctgggttttccggtactgggcgcaacctctggtctgattggttgtatggcagcactggaagccgttaaatggattaccgacgcaggtaacctgttcaccgatcgtatgtaccgcatgaacgtcctggatatgagcagctgtaccatcgcagtcaaacgtaatccgcgttgtccgtgttgtggtaccggcggcgataccgacgaaagcgtggcatatctgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1796014_sequence/1
type: Sequence
persistent identity: BBa K1796014 sequence
display id: BBa_K1796014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcggaacgggaggggatacagatgagccgtctgcatatttgcgataccaccctgcgtgacggcgaacaagcaccgggcgttgcgtttagcgcggaagagaaaaccgaaatcgcgattatgctggatagcgcaggcgttgaacaggcagaaattggcattccggcaatgggtaaaaccgagtgccgtagcattgcacgcattgcagctctgggtctgcaaatgaaactgatgacctggaaccgcgcagtttttaccgatatcgatgcaaccgaaagtaccggcgtaggttgggcacatattagcgttccggttagcaccgttcagatgaaatccaaactgggcatgaacccggaacaggttaccgaactgatccgcaaaagcgttgactacgcactgtgcaaaggtctgaccgttagcgttggttttgaagacgcaagtcgcgcagacgacctgtttctggaacaactggcaaaccagctgtatcgcgacggtattcgtcgttttcgctacgcagataccctgagcgttcatcatccggcagcaattgcagcacgtattgatcgtctggtttctcgcgttccgcaagacgttgaactggagatccactgccacaacgattacggtctggcactggcaaataccctggcagcactgcaagcaggcgcagtttgggcaagtaccaccgtttctggtctgggcgaacgcgcaggtaataccgcactggaagaggttgtcatgagctggcgcgatctgtatcagggtacctgttctgttcgtccggaactgctgaatccgctggcagcactggttagcaaagcgagcaatcgcattatcccggaaggtaaaccgattgtcggcgatatggtatttgcgcacgaaagcggcatccatattaacggtctgctgaaagaacgcgcagcatatcaagcactggatccgaccgaactgggtaccgatcatagctttgtcctgggcaaacactctggtcgttctgctgtccagtatatgctggaacaggaaggtattgaagcaggttctggcgagatcaaattcctgctggaacgtctgcgtctggttggcgaagatccgaaacgcgttatccattctgcagatctgcgtcgttggctgcaatattatccggcagaactgccgaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z