LOCUS       BBa_K1796016            3280 bp    DNA     linear       26-Nov-2024
DEFINITION  dinitrogease
ACCESSION   BBa_K1796016
VERSION     BBa_K1796016.1  
FEATURES             Location/Qualifiers
     misc_feature    62..67
                     /label=-35
     misc_feature    85..90
                     /label=-10
     promoter        1..100
                     /label=Pnif
     promoter        1..100
                     /label=BBa_K1796001
     CDS             130..1581
                     /label=nifD coding sequence
     RBS             109..129
                     /label=unknown rbs
     CDS             109..1581
                     /label=BBa_K1796008
     CDS             1611..3143
                     /label=nifK coding sequence
     RBS             1590..1610
                     /label=uknown rbs
     CDS             1590..3143
                     /label=BBa_K1796009
     stem_loop       3163..3206
                     /label=stem_loop
     misc_feature    3152..3231
                     /label=BBa_B0010
     terminator      3152..3231
                     /label=BBa_B0010
     stem_loop       3247..3266
                     /label=T7 TE
     misc_feature    3273..3273
                     /label=stop
     polyA_site      3267..3280
                     /label=polya
     misc_feature    3240..3280
                     /label=BBa_B0012
     terminator      3240..3280
                     /label=BBa_B0012
     terminator      3152..3280
                     /label=BBa_B0015
ORIGIN
        1 cgtaaaattt gacacatatg tgaattgagg ataaatgtca gggatttcat ggagaagtga
       61 attgactgta tttgtccctg tctctaagat gtaattatat tactagagca ggagggagga
      121 ataggccaaa tgagcagcat cgtcgacaaa ggcaaacaga tcgtggaaga aatcctggaa
      181 gtctacccga aaaaagcgaa aaaagaccgc accaaacact tcgagatcgc ggacgaagaa
      241 ctggttaact gcggtacctg cagcatcaaa agcaacatga aaagtcgtcc gggcgttatg
      301 acggcacgtg gttgtgcata cgcaggttca aaaggcgttg tttggggtcc gatcaaagac
      361 atggtccata ttagccacgg tccgattggt tgcggtcaat attcttgggg tacccgtcgt
      421 aactacgcaa acggtatcct gggcatcgat aacttcaccg caatgcagat caccagcaac
      481 ttccaggaaa aagacatcgt cttcggcggc gacaaaaaac tggaagtcat ctgccgcgaa
      541 atcaaagaga tgtttccgct ggcgaaaggc attagcgttc aatctgagtg tccggttggt
      601 ctgattggcg acgatattgg cgcggtcgcc aaaaagatga ccgaagaact gggcattccg
      661 gttattccgg ttcgttgcga aggttttcgc ggggttagtc agagcctggg tcatcatatt
      721 gcaaacgacg caattcgcga ttttctgatg ggccgtcgcg aactgaaaga gtgcggtccg
      781 tacgacgtta gcatcattgg cgattacaac attggcggcg atgcttgggc aagtcgtatt
      841 ctgctggaag aaatgggtct gcgcgttatt gcacagtggt ctggcgacgg taccattaac
      901 gaactgggta tcgcgcacaa aagcaaactg aacctgatcc actgccatcg ttccatgaac
      961 tacatgtgca ccaccatgga acaggaatac ggtatcccgt ggatggaata caacttcttc
     1021 ggtccgacca aaaccatgga aagcctgcgc gcaattgcag cgcgttttga cgaaaccatc
     1081 caggagaaat gcgagcaggt tattgcccag tatatgccgc agatggaggc ggtcattcgt
     1141 aaatatcgcc cgcgtctgga gggcaaaaaa gttatgctgc tgattggcgg tctgcgcgca
     1201 cgtcatacca ttggcgcata cgaagatctg ggcatggaaa ttgttgcgac cggttacgaa
     1261 tttgcgcaca aagacgacta cgagaaaacc ttcccggacg tcaaagaggg caccattctg
     1321 tacgacgatc cgaccgcata cgaactggaa gaactggcac agcgtctgaa cattgatctg
     1381 atgggcgcag gcgtcaaaga gaaatacgtc taccacaaaa tgggcattcc gttccgtcag
     1441 atgcatagct gggattatag cggtccgtat cacggtttcg acggctttaa aatcttcgcc
     1501 cgcgatatgg atatgaccat caacagcccg gtttggagtc tgctgccgtc tcgtcaaacc
     1561 gcagaagttc cggtttaata atactagagg cagactgcgg aggtgccggt atgagcgaac
     1621 gtccgaacat cgttgatcac aaccagctgt tccgccagga taaatacgtc cgtcaacgcg
     1681 aagagaaacg cgcttttgaa gcaccgtgta gtccggaaga agttaccgat accctggagt
     1741 acaccaaaac caaagagtac aaagacaaaa actttgcgcg caccgcggtt gttgttaatc
     1801 cggcaaaagc ctgtcaaccg ttaggcgcag ttatggcagc actgggtttt gagaaaaccc
     1861 tgccgtttat tcacggctct cagggttgta ccgcatattt tcgcagccat ctggcacgtc
     1921 atttcaaaga accggttccg gctgtttcta ccagtatgac ggaagacgca gctgtttttg
     1981 gcggtatgcg taacctgatc gacggcattg aaaactgcat tgcgctgtac cagccggaaa
     2041 tgattgcagt ttgcaccacc tgcatggcag aagttattgg cgacgatctg agcgcatttc
     2101 tggcaaacgc acgtcaagaa ggcgttttac cggaagatat gccggttccg tttgcaaaca
     2161 ccccgagttt tagcggtagc catatcaccg gctacgacgc aatgttacgt agcgtcctgg
     2221 aaaccctgta caacaaaagc ggtcgtaccg cacaaccggg tcacgaatta aaactgaacg
     2281 tcctgctggg ctttgacggt tataccggta acttcgcgga aatgcgtcgt atgctgggta
     2341 tgtttggggc aacctatacc atcctgggcg atcacagcag caactttgat tctggcgcga
     2401 ccggcgaata tagctattat tacggtggta ccccgttaga agacgttccg aaagcagcag
     2461 acgcagcagg taccttagca attcagcagt acagcctgcg taaaaccctg ggctatatga
     2521 aacagacctg gggtcagcaa gttagcagca tttctacccc gctgggtatt cgcgcaaccg
     2581 atcgcttact ggaagaaatt agccgtttaa gcggtcgcga aattccggaa gcactgaaac
     2641 aggaacgcgc acgtattgtt gacgctatga tggatagcca cgcatatctg cacggtaaac
     2701 gcgttgcaat ggcaggcgat ccggatatgt taatcggtct gatcggcttc tgcttagaac
     2761 tgggcatgga accggtacat atcgtttgca gcaacggcga tcgcaaattc gagaaagaag
     2821 cggaactgct gctgaaatcc tctccgtatg gcgcagaagc aaccgttcat tctggtcagg
     2881 atctgtggca tatgcgtagc ctgctgtttc aagatccggt tgatctggct attggtagca
     2941 gccatctgaa atttgcggcg aaagaggcgg aaattccgtt actgcgcgtt ggttttccga
     3001 tctttgatcg ccatcacctg caccgttatc cgattattgg ctatcagggc gcactgaatc
     3061 tgctgaccca gtttgtcaac accatcctgg acgtcatgga agaacaagca ccggatcata
     3121 gctttgactt agttcgttaa taatactaga gccaggcatc aaataaaacg aaaggctcag
     3181 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     3241 cacactggct caccttcggg tgggcctttc tgcgtttata
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