LOCUS BBa_K1797004 4750 bp DNA linear 22-Dec-2024 DEFINITION Blue Light Sensing System With GFP And BFP Reporter ACCESSION BBa_K1797004 VERSION BBa_K1797004.1 FEATURES Location/Qualifiers promoter 1..250 /label=FixK2 promoter promoter 1..250 /label=BBa_K592006 misc_feature 263..266 /label=conserved RBS 259..270 /label=BBa_B0034 CDS 280..987 /label=cI lambda misc_feature 988..1020 /label=LVA misc_feature 1027..1051 /label=Help:Barcodes CDS 277..1026 /label=BBa_C0051 stem_loop 1071..1114 /label=stem_loop misc_feature 1060..1139 /label=BBa_B0010 terminator 1060..1139 /label=BBa_B0010 stem_loop 1155..1174 /label=T7 TE misc_feature 1181..1181 /label=stop polyA_site 1175..1188 /label=polya misc_feature 1148..1188 /label=BBa_B0012 terminator 1148..1188 /label=BBa_B0012 promoter 1234..1239 /label=-10 promoter 1211..1216 /label=-35 misc_feature 1221..1237 /label=OR1 misc_feature 1197..1213 /label=OR2 misc_feature 1197..1245 /label=BBa_R0051 promoter 1197..1245 /label=BBa_R0051 misc_feature 259..1245 /label=BBa_Q04510 misc_feature 1258..1261 /label=conserved RBS 1254..1265 /label=BBa_B0034 CDS 1272..1937 /label=amilCP CDS 1272..1940 /label=BBa_K592009 misc_feature 1..1940 /label=BBa_K592020 promoter 1949..1983 /label=BBa_J23104 misc_feature 1996..1999 /label=conserved RBS 1992..2003 /label=BBa_B0034 CDS 2010..3140 /label=YF1 CDS 2010..3140 /label=BBa_K592004 misc_feature 3153..3156 /label=conserved RBS 3149..3160 /label=BBa_B0034 CDS 3167..3787 /label=FixJ CDS 3167..3787 /label=BBa_K592005 promoter 3796..4045 /label=FixK2 promoter promoter 3796..4045 /label=BBa_K592006 CDS 4052..4747 /label=amilGFP CDS 4052..4750 /label=BBa_K592010 misc_feature 1949..4750 /label=BBa_K1797000 ORIGIN 1 gccggagccg attatccgca cccgtccttg gtcttggaca cactcgctcg cgatccgaag 61 ccgcctttca aagatactcc gcagtgaaac gcatctttta agcgcgactt gtcgccttcg 121 cgcagcagaa cactcgtatg gcactcaatc catgatcgct tcgttcgctc cgacgcgcgt 181 tgcggggccc ccctcgaccg gatgacaaca cattgcgtca cctcaactgc tgcgcgcgca 241 ctgcgtcaca tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta 301 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat 361 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt 421 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa 481 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg 541 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct 601 catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag 661 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt 721 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc 781 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat 841 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac 901 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct 961 agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct 1021 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa 1081 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 1141 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtaac 1201 accgtgcgtg ttgactattt tacctctggc ggtgataatg gttgctacta gagaaagagg 1261 agaaatacta gatgagtgtg atcgctaaac aaatgaccta caaggtttat atgtcaggca 1321 cggtcaatgg acactacttt gaggtcgaag gcgatggaaa aggtaagccc tacgaggggg 1381 agcagacggt aaagctcact gtcaccaagg gcggacctct gccatttgct tgggatattt 1441 tatcaccaca gtgtcagtac ggaagcatac cattcaccaa gtaccctgaa gacatccctg 1501 actatgtaaa gcagtcattc ccggagggct atacatggga gaggatcatg aactttgaag 1561 atggtgcagt gtgtactgtc agcaatgatt ccagcatcca aggcaactgt ttcatctacc 1621 atgtcaagtt ctctggtttg aactttcctc ccaatggacc tgtcatgcag aagaagacac 1681 agggctggga acccaacact gagcgtctct ttgcacgaga tggaatgctg ctaggaaaca 1741 actttatggc tctgaagtta gaaggaggcg gtcactattt gtgtgaattt aaaactactt 1801 acaaggcaaa gaagcctgtg aagatgccag ggtatcacta tgttgaccgc aaactggatg 1861 taaccaatca caacaaggat tacacttcgg ttgagcagtg tgaaatttcc attgcacgca 1921 aacctgtggt cgcctaataa tactagagtt gacagctagc tcagtcctag gtattgtgct 1981 agctactaga gaaagaggag aaatactaga tggctagttt tcaatcattt gggataccag 2041 gacagctgga agtcatcaaa aaagcacttg atcacgtgcg agtcggtgtg gtaattacag 2101 atcccgcact tgaagataat cctattgtct acgtaaatca aggctttgtt caaatgaccg 2161 gctacgagac cgaggaaatt ttaggaaaga actgtcgctt cttacagggg aaacacacag 2221 atccggcaga agtggacaac atcagaaccg ctttacaaaa taaagaaccg gtcaccgttc 2281 agatccaaaa ctacaaaaaa gacggaacga tgttctggaa tgaattaaat attgatccaa 2341 tggaaataga ggataaaacg tattttgtcg gcattcagaa tgatatcacc gagcaccagc 2401 agacccaggc gcgtctccag gaactgcaat ccgagctcgt ccacgtctcc aggctgagcg 2461 ccatgggcga aatggcgtcc gcgctcgcgc acgagctcaa ccagccgctg gcggcgatca 2521 gcaactacat gaagggctcg cggcggctgc ttgccggcag cagtgatccg aacacaccga 2581 aggtcgaaag cgccctggac cgcgccgccg agcaggcgct gcgcgccggc cagatcatcc 2641 ggcgcctgcg cgacttcgtt gcccgcggcg aatcggagaa gcgggtcgag agtctctcca 2701 agctgatcga ggaggccggc gcgctcgggc ttgccggcgc gcgcgagcag aacgtgcagc 2761 tccgcttcag tctcgatccg ggcgccgatc tcgttctcgc cgaccgggtg cagatccagc 2821 aggtcctggt caacctgttc cgcaacgcgc tggaagcgat ggctcagtcg cagcgacgcg 2881 agctcgtcgt caccaacacc cccgccgccg acgacatgat cgaggtcgaa gtgtccgaca 2941 ccggcagcgg tttccaggac gacgtcattc cgaacctgtt tcagactttc ttcaccacca 3001 aggacaccgg catgggcgtg ggactgtcca tcagccgctc gatcatcgaa gctcacggcg 3061 ggcgcatgtg ggccgagagc aacgcatcgg gcggggcgac cttccgcttc accctcccgg 3121 cagccgacga gaattaataa tactagagaa agaggagaaa tactagatga cgaccaaggg 3181 acatatctac gtcatcgacg acgacgcggc gatgcgggat tcgctgaatt tcctgctgga 3241 ttctgccggc ttcggcgtca cgctgtttga cgacgcgcaa gcctttctcg acgccctgcc 3301 gggtctctcc ttcggctgcg tcgtctccga cgtgcgcatg ccgggccttg acggcatcga 3361 gctgttgaag cggatgaagg cgcagcaaag cccctttccg atcctcatca tgaccggtca 3421 cggcgacgtg ccgctcgcgg tcgaggcgat gaagttaggg gcggtcgact ttctggaaaa 3481 gcctttcgag gacgaccgcc tcaccgccat gatcgaatcg gcgatccgcc aggccgagcc 3541 ggccgccaag agcgaggccg tcgcgcagga tatcgccgcc cgcgtcgcct cgttgagccc 3601 cagggagcgc caggtcatgg aagggctgat cgccggcctt tccaacaagc tgatcgcccg 3661 cgagtacgac atcagcccgc gcaccatcga ggtgtatcgg gccaacgtca tgaccaagat 3721 gcaggccaac agcctttcgg agctggttcg cctcgcgatg cgcgccggca tgctcaacga 3781 ttaataatac tagaggccgg agccgattat ccgcacccgt ccttggtctt ggacacactc 3841 gctcgcgatc cgaagccgcc tttcaaagat actccgcagt gaaacgcatc ttttaagcgc 3901 gacttgtcgc cttcgcgcag cagaacactc gtatggcact caatccatga tcgcttcgtt 3961 cgctccgacg cgcgttgcgg ggcccccctc gaccggatga caacacattg cgtcacctca 4021 actgctgcgc gcgcactgcg tcacatacta gatgtcttat tcaaagcatg gcatcgtaca 4081 agaaatgaag acgaaatacc atatggaagg cagtgtcaat ggccatgaat ttacgatcga 4141 aggtgtagga actgggtacc cttacgaagg gaaacagatg tccgaattag tgatcatcaa 4201 gcctgcggga aaaccccttc cattctcctt tgacatactg tcatcagtct ttcaatatgg 4261 aaaccgttgc ttcacaaagt acccggcaga catgcctgac tatttcaagc aagcattccc 4321 agatggaatg tcatatgaaa ggtcatttct atttgaggat ggagcagttg ctacagccag 4381 ctggaacatt cgtctcgaag gaaattgctt catccacaaa tccatctttc atggcgtaaa 4441 ctttcccgct gatggacccg taatgaaaaa gaagacaatt gactgggata agtccttcga 4501 aaaaatgact gtgtctaaag aggtgctaag aggtgacgtg actatgtttc ttatgctcga 4561 aggaggtggt tctcacagat gccaatttca ctccacttac aaaacagaga agccggtcac 4621 actgcccccg aatcatgtcg tagaacatca aattgtgagg accgaccttg gccaaagtgc 4681 aaaaggcttt acagtcaagc tggaagcaca tgccgcggct catgttaacc ctttgaaggt 4741 taaataataa //