BBa_K1797005 1 BBa_K1797005 sgRNA 2015-09-16T11:00:00Z 2015-09-18T07:00:57Z No. This part includes sgRNA, which can be used to do site-specific gene editing when CRISPR Cas9 system is present. Users can use BspQI to digest the part and ligate with a DNA sequence that is targeted at a certain gene locus. false false _2222_ 27902 27902 9 false No. false iGEM15_Tsinghua annotation2471408 1 sgRNA changeable region range2471408 1 176 198 annotation2471407 1 sgRNA scaffold range2471407 1 199 274 annotation2466642 1 sgRNA range2466642 1 176 281 annotation2466648 1 ptet promoter range2466648 1 108 178 BBa_K1797005_sequence 1 gtacggccgcctccaggtcccgggggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaaatctaaagatcttttaattcctaatttttgttgacactctatcgttgatagagttattttaccactccctatcagtgatagagaaaatgaagagcagctgctcttcagttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttttgaagcttgggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacggtctccagcttggctgttttggcggatgagagaagattttcagcctgatacagattaaatcagaacgcagaagcggtctgataaaacagaatttgcctggcggcagtagcgcggtggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaactggatcctgccgggctggccggccacta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z