LOCUS BBa_K1797015 5660 bp DNA linear 22-Nov-2024 DEFINITION dCas9-Long Linker (Gly-Gly-Ser-Gly)x3-Bxb1 gp35 ACCESSION BBa_K1797015 VERSION BBa_K1797015.1 FEATURES Location/Qualifiers CDS 1..4107 /label=dCas9 CDS 1..4107 /label=BBa_K1797006 misc_feature 4116..4151 /label=Long Linker misc_feature 4116..4151 /label=BBa_K243006 CDS 4158..5660 /label=Mycobacterium phage Bxb1 integrase CDS CDS 4158..5660 /label=BBa_K907000 ORIGIN 1 atggataaga aatactcaat aggcttagct atcggcacaa atagcgtcgg atgggcggtg 61 atcactgatg aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc 121 cacagtatca aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa 181 gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt 241 tatctacagg agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga 301 cttgaagagt cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga 361 aatatagtag atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa 421 aaattggtag attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat 481 atgattaagt ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat 541 gtggacaaac tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct 601 attaacgcaa gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga 661 cgattagaaa atctcattgc tcagctcccc ggtgagaaga aaaatggctt atttgggaat 721 ctcattgctt tgtcattggg tttgacccct aattttaaat caaattttga tttggcagaa 781 gatgctaaat tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg 841 caaattggag atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt 901 ttactttcag atatcctaag agtaaatact gaaataacta aggctcccct atcagcttca 961 atgattaaac gctacgatga acatcatcaa gacttgactc ttttaaaagc tttagttcga 1021 caacaacttc cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa cggatatgca 1081 ggttatattg atgggggagc tagccaagaa gaattttata aatttatcaa accaatttta 1141 gaaaaaatgg atggtactga ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc 1201 aagcaacgga cctttgacaa cggctctatt ccccatcaaa ttcacttggg tgagctgcat 1261 gctattttga gaagacaaga agacttttat ccatttttaa aagacaatcg tgagaagatt 1321 gaaaaaatct tgacttttcg tattccttat tatgttggtc cattggcgcg tggcaatagt 1381 cgttttgcat ggatgactcg gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa 1441 gttgtcgata aaggtgcttc agctcaatca tttattgaac gcatgacaaa ctttgataaa 1501 aatcttccaa atgaaaaagt actaccaaaa catagtttgc tttatgagta ttttacggtt 1561 tataacgaat tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc agcatttctt 1621 tcaggtgaac agaagaaagc cattgttgat ttactcttca aaacaaatcg aaaagtaacc 1681 gttaagcaat taaaagaaga ttatttcaaa aaaatagaat gttttgatag tgttgaaatt 1741 tcaggagttg aagatagatt taatgcttca ttaggtacct accatgattt gctaaaaatt 1801 attaaagata aagatttttt ggataatgaa gaaaatgaag atatcttaga ggatattgtt 1861 ttaacattga ccttatttga agatagggag atgattgagg aaagacttaa aacatatgct 1921 cacctctttg atgataaggt gatgaaacag cttaaacgtc gccgttatac tggttgggga 1981 cgtttgtctc gaaaattgat taatggtatt agggataagc aatctggcaa aacaatatta 2041 gattttttga aatcagatgg ttttgccaat cgcaatttta tgcagctgat ccatgatgat 2101 agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg cgatagttta 2161 catgaacata ttgcaaattt agctggtagc cctgctatta aaaaaggtat tttacagact 2221 gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc ataagccaga aaatatcgtt 2281 attgaaatgg cacgtgaaaa tcagacaact caaaagggcc agaaaaattc gcgagagcgt 2341 atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa agagcatcct 2401 gttgaaaata ctcaattgca aaatgaaaag ctctatctct attatctcca aaatggaaga 2461 gacatgtatg tggaccaaga attagatatt aatcgtttaa gtgattatga tgtcgatgcc 2521 attgttccac aaagtttcct taaagacgat tcaatagaca ataaggtctt aacgcgttct 2581 gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa 2641 aactattgga gacaacttct aaacgccaag ttaatcactc aacgtaagtt tgataattta 2701 acgaaagctg aacgtggagg tttgagtgaa cttgataaag ctggttttat caaacgccaa 2761 ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa ttttggatag tcgcatgaat 2821 actaaatacg atgaaaatga taaacttatt cgagaggtta aagtgattac cttaaaatct 2881 aaattagttt ctgacttccg aaaagatttc caattctata aagtacgtga gattaacaat 2941 taccatcatg cccatgatgc gtatctaaat gccgtcgttg gaactgcttt gattaagaaa 3001 tatccaaaac ttgaatcgga gtttgtctat ggtgattata aagtttatga tgttcgtaaa 3061 atgattgcta agtctgagca agaaataggc aaagcaaccg caaaatattt cttttactct 3121 aatatcatga acttcttcaa aacagaaatt acacttgcaa atggagagat tcgcaaacgc 3181 cctctaatcg aaactaatgg ggaaactgga gaaattgtct gggataaagg gcgagatttt 3241 gccacagtgc gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa aacagaagta 3301 cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt 3361 gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct 3421 tattcagtcc tagtggttgc taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt 3481 aaagagttac tagggatcac aattatggaa agaagttcct ttgaaaaaaa tccgattgac 3541 tttttagaag ctaaaggata taaggaagtt aaaaaagact taatcattaa actacctaaa 3601 tatagtcttt ttgagttaga aaacggtcgt aaacggatgc tggctagtgc cggagaatta 3661 caaaaaggaa atgagctggc tctgccaagc aaatatgtga attttttata tttagctagt 3721 cattatgaaa agttgaaggg tagtccagaa gataacgaac aaaaacaatt gtttgtggag 3781 cagcataagc attatttaga tgagattatt gagcaaatca gtgaattttc taagcgtgtt 3841 attttagcag atgccaattt agataaagtt cttagtgcat ataacaaaca tagagacaaa 3901 ccaatacgtg aacaagcaga aaatattatt catttattta cgttgacgaa tcttggagct 3961 cccgctgctt ttaaatattt tgatacaaca attgatcgta aacgatatac gtctacaaaa 4021 gaagttttag atgccactct tatccatcaa tccatcactg gtctttatga aacacgcatt 4081 gatttgagtc agctaggagg tgactaatac tagagggtgg ttctggtggt ggttctggtg 4141 gtggttctgg ttactagatg agagccctgg tagtcatccg cctgtcccgc gtcaccgatg 4201 ctacgacttc accggagcgt cagctggagt cttgccagca gctctgcgcc cagcgcggct 4261 gggacgtcgt cggggtagcg gaggatctgg acgtctccgg ggcggtcgat ccgttcgacc 4321 ggaagcgcag accgaacctg gcccggtggc tagcgttcga ggagcaaccg ttcgacgtga 4381 tcgtggcgta ccgggtagac cggttgaccc gatcgatccg gcatctgcaa cagctggtcc 4441 actgggccga ggaccacaag aagctggtcg tctccgcgac cgaagcgcac ttcgatacga 4501 cgacgccgtt tgcggcggtc gtcatcgcgc ttatgggaac ggtggcgcag atggaattag 4561 aagcgatcaa agagcggaac cgttcggctg cgcatttcaa tatccgcgcc gggaaatacc 4621 gaggatccct gccgccgtgg ggatacctgc ctacgcgcgt ggacggggag tggcggctgg 4681 tgccggaccc tgtgcagcga gagcgcatcc tcgaggtgta tcaccgcgtc gtcgacaacc 4741 acgagccgct gcacctggtg gcccacgacc tgaaccggcg tggtgtcctg tcgccgaagg 4801 actacttcgc gcagctgcaa ggccgcgagc cgcagggccg ggagtggtcg gctaccgcgc 4861 tgaagcgatc gatgatctcc gaggcgatgc tcgggtacgc gactctgaac ggtaagaccg 4921 tccgagacga cgacggagcc ccgctggtgc gggctgagcc gatcctgacc cgtgagcagc 4981 tggaggcgct gcgcgccgag ctcgtgaaga cctcccgggc gaagcccgcg gtgtctaccc 5041 cgtcgctgct gctgcgggtg ttgttctgcg cggtgtgcgg ggagcccgcg tacaagttcg 5101 ccgggggagg acgtaagcac ccgcgctacc gctgccgctc gatggggttc ccgaagcact 5161 gcgggaacgg cacggtggcg atggccgagt gggacgcgtt ctgcgaggag caggtgctgg 5221 atctgctcgg ggacgcggag cgtctggaga aagtctgggt agccggctcg gactccgcgg 5281 tcgaactcgc ggaggtgaac gcggagctgg tggacctgac gtcgctgatc ggctccccgg 5341 cctaccgggc cggctctccg cagcgagaag cactggatgc ccgtattgcg gcgctggccg 5401 cgcggcaaga ggagctggag ggcctagagg ctcgcccgtc tggctgggag tggcgcgaga 5461 ccgggcagcg gttcggggac tggtggcggg agcaggacac cgcggcaaag aacacctggc 5521 ttcggtcgat gaacgttcgg ctgacgttcg acgtccgcgg cgggctgact cgcacgatcg 5581 acttcgggga tctgcaagag tacgagcagc atctcaggct cggcagcgtg gtcgaacggc 5641 tacacaccgg gatgtcgtag //