BBa_K1429001 1 BBa_K1429001 Red Fluorescent Protein (RFP) "Rudolph" coding region, intellectual property-free 2014-09-22T11:00:00Z 2015-05-08T01:10:23Z DNA 2.0 ProteinPaintbox An intellectual property free protein coding region which may be used by any scientist to create a biological machine. false false _1807_ 0 22579 9 In stock true n/a false Ramsez McCall BBa_J37019 1 BBa_J37019 AHL induced LuxR generator 2006-07-18T11:00:00Z 2015-08-31T04:08:48Z N/A Released HQ 2013 N/A false false _66_ 0 1064 66 In stock false N/A false Deepti Aswani component2245214 1 BBa_R0062 component2245223 1 BBa_C0062 component2245220 1 BBa_B0034 annotation2245223 1 BBa_C0062 range2245223 1 82 837 annotation2245220 1 BBa_B0034 range2245220 1 64 75 annotation2245214 1 BBa_R0062 range2245214 1 1 55 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1799018 1 BBa_K1799018 AHL-Induced RFP Generator 2015-08-26T11:00:00Z 2015-09-18T04:11:54Z This part was synthesized, and is based on E. coli genomic data retrieved via EcoGene. In the presence of AHL, generates LuxR and RFP. false false _2224_ 9151 26954 9 true None false Will Shindel component2438771 1 BBa_J37019 component2438772 1 BBa_K1429001 annotation2438772 1 BBa_K1429001 range2438772 1 871 1573 annotation2438771 1 BBa_J37019 range2438771 1 1 862 BBa_R0062 1 lux pR Promoter (luxR & HSL regulated -- lux pR) 2003-01-31T12:00:00Z 2015-05-08T01:14:15Z <em>V. fischeri</em> Released HQ 2013 Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription. false true _1_ 0 24 7 In stock false <P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation2045 1 LuxR/HSL range2045 1 1 20 annotation2047 1 -10 range2047 1 42 47 annotation7070 1 BBa_R0062 range7070 1 1 55 annotation2048 1 start range2048 1 53 53 annotation2046 1 -35 range2046 1 20 25 BBa_C0062 1 luxr luxR repressor/activator, (no LVA?) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z <em>V. fischeri</em> <genbank>AF170104</genbank> Released HQ 2013 In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p> false true _1_ 0 24 7 In stock false <P> <P>2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in <em>E. coli</em> with codons used at a similar frequency. <P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation2213986 1 Help:Barcodes range2213986 1 757 781 annotation1766 1 luxR range1766 1 1 750 annotation1764 1 T range1764 1 174 174 annotation1765 1 A range1765 1 492 492 annotation1762 1 prefix range1762 1 1 2 annotation7039 1 BBa_C0062 range7039 1 1 756 BBa_R0062_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa BBa_J37019_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac BBa_B0034_sequence 1 aaagaggagaaa BBa_K1799018_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagcgagaccttaggaggtaaacatatgtccctgtcgaaacaagtactgccacacgatgttaagatgcgctatcatatggatggctgcgttaatggtcattctttcaccattgagggtgaaggtgcaggcaaaccgtatgagggcaagaagatcttggaactgcgcgtgacgaaaggtggcccgctgccttttgcgttcgatatcctgagcagcgtttttacctacggtaaccgttgtttttgcgagtatccagaggacatgccggactactttaaacagagcctgccggaaggtcattcttgggaacgcaccctgatgtttgaggatggcggttgtggtacggcgagcgcgcacatttccctggacaagaactgcttcgtgcacaagagcaccttccacggcgtcaatttcccggcaaacggtccggtcatgcaaaagaaagctatgaactgggagccgagcagcgaactgattacggcgtgcgacggtatcctgaaaggcgatgtgaccatgtttctgttgctggaaggtggccaccgtcttaaatgtcagttcaccaccagctacaaagcccacaaggcagttaagatgccgccgaatcacattatcgaacacgtgcttgttaaaaaagaggttgccgacggctttcagatccaagagcatgcggtcgcaaagcacttcaccgtcgacgttaaagaaacgtaa BBa_K1429001_sequence 1 cgagaccttaggaggtaaacatatgtccctgtcgaaacaagtactgccacacgatgttaagatgcgctatcatatggatggctgcgttaatggtcattctttcaccattgagggtgaaggtgcaggcaaaccgtatgagggcaagaagatcttggaactgcgcgtgacgaaaggtggcccgctgccttttgcgttcgatatcctgagcagcgtttttacctacggtaaccgttgtttttgcgagtatccagaggacatgccggactactttaaacagagcctgccggaaggtcattcttgggaacgcaccctgatgtttgaggatggcggttgtggtacggcgagcgcgcacatttccctggacaagaactgcttcgtgcacaagagcaccttccacggcgtcaatttcccggcaaacggtccggtcatgcaaaagaaagctatgaactgggagccgagcagcgaactgattacggcgtgcgacggtatcctgaaaggcgatgtgaccatgtttctgttgctggaaggtggccaccgtcttaaatgtcagttcaccaccagctacaaagcccacaaggcagttaagatgccgccgaatcacattatcgaacacgtgcttgttaaaaaagaggttgccgacggctttcagatccaagagcatgcggtcgcaaagcacttcaccgtcgacgttaaagaaacgtaa BBa_C0062_sequence 1 atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z