BBa_J37019
1
BBa_J37019
AHL induced LuxR generator
2006-07-18T11:00:00Z
2015-08-31T04:08:48Z
N/A
Released HQ 2013
N/A
false
false
_66_
0
1064
66
In stock
false
N/A
false
Deepti Aswani
component2245223
1
BBa_C0062
component2245220
1
BBa_B0034
component2245214
1
BBa_R0062
annotation2245214
1
BBa_R0062
range2245214
1
1
55
annotation2245223
1
BBa_C0062
range2245223
1
82
837
annotation2245220
1
BBa_B0034
range2245220
1
64
75
BBa_R0062
1
lux pR
Promoter (luxR & HSL regulated -- lux pR)
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
<em>V. fischeri</em>
Released HQ 2013
Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
false
true
_1_
0
24
7
In stock
false
<P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation2045
1
LuxR/HSL
range2045
1
1
20
annotation2047
1
-10
range2047
1
42
47
annotation2046
1
-35
range2046
1
20
25
annotation2048
1
start
range2048
1
53
53
annotation7070
1
BBa_R0062
range7070
1
1
55
BBa_C0062
1
luxr
luxR repressor/activator, (no LVA?)
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
<em>V. fischeri</em> <genbank>AF170104</genbank>
Released HQ 2013
In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p>
false
true
_1_
0
24
7
In stock
false
<P> <P>2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in <em>E. coli</em> with codons used at a similar frequency. <P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation1762
1
prefix
range1762
1
1
2
annotation7039
1
BBa_C0062
range7039
1
1
756
annotation2213986
1
Help:Barcodes
range2213986
1
757
781
annotation1764
1
T
range1764
1
174
174
annotation1765
1
A
range1765
1
492
492
annotation1766
1
luxR
range1766
1
1
750
BBa_K1799018
1
BBa_K1799018
AHL-Induced RFP Generator
2015-08-26T11:00:00Z
2015-09-18T04:11:54Z
This part was synthesized, and is based on E. coli genomic data retrieved via EcoGene.
In the presence of AHL, generates LuxR and RFP.
false
false
_2224_
9151
26954
9
true
None
false
Will Shindel
component2438772
1
BBa_K1429001
component2438771
1
BBa_J37019
annotation2438771
1
BBa_J37019
range2438771
1
1
862
annotation2438772
1
BBa_K1429001
range2438772
1
871
1573
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1429001
1
BBa_K1429001
Red Fluorescent Protein (RFP) "Rudolph" coding region, intellectual property-free
2014-09-22T11:00:00Z
2015-05-08T01:10:23Z
DNA 2.0 ProteinPaintbox
An intellectual property free protein coding region which may be used by any scientist to create a biological machine.
false
false
_1807_
0
22579
9
In stock
true
n/a
false
Ramsez McCall
BBa_R0062_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
BBa_J37019_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1799018_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagcgagaccttaggaggtaaacatatgtccctgtcgaaacaagtactgccacacgatgttaagatgcgctatcatatggatggctgcgttaatggtcattctttcaccattgagggtgaaggtgcaggcaaaccgtatgagggcaagaagatcttggaactgcgcgtgacgaaaggtggcccgctgccttttgcgttcgatatcctgagcagcgtttttacctacggtaaccgttgtttttgcgagtatccagaggacatgccggactactttaaacagagcctgccggaaggtcattcttgggaacgcaccctgatgtttgaggatggcggttgtggtacggcgagcgcgcacatttccctggacaagaactgcttcgtgcacaagagcaccttccacggcgtcaatttcccggcaaacggtccggtcatgcaaaagaaagctatgaactgggagccgagcagcgaactgattacggcgtgcgacggtatcctgaaaggcgatgtgaccatgtttctgttgctggaaggtggccaccgtcttaaatgtcagttcaccaccagctacaaagcccacaaggcagttaagatgccgccgaatcacattatcgaacacgtgcttgttaaaaaagaggttgccgacggctttcagatccaagagcatgcggtcgcaaagcacttcaccgtcgacgttaaagaaacgtaa
BBa_K1429001_sequence
1
cgagaccttaggaggtaaacatatgtccctgtcgaaacaagtactgccacacgatgttaagatgcgctatcatatggatggctgcgttaatggtcattctttcaccattgagggtgaaggtgcaggcaaaccgtatgagggcaagaagatcttggaactgcgcgtgacgaaaggtggcccgctgccttttgcgttcgatatcctgagcagcgtttttacctacggtaaccgttgtttttgcgagtatccagaggacatgccggactactttaaacagagcctgccggaaggtcattcttgggaacgcaccctgatgtttgaggatggcggttgtggtacggcgagcgcgcacatttccctggacaagaactgcttcgtgcacaagagcaccttccacggcgtcaatttcccggcaaacggtccggtcatgcaaaagaaagctatgaactgggagccgagcagcgaactgattacggcgtgcgacggtatcctgaaaggcgatgtgaccatgtttctgttgctggaaggtggccaccgtcttaaatgtcagttcaccaccagctacaaagcccacaaggcagttaagatgccgccgaatcacattatcgaacacgtgcttgttaaaaaagaggttgccgacggctttcagatccaagagcatgcggtcgcaaagcacttcaccgtcgacgttaaagaaacgtaa
BBa_C0062_sequence
1
atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z