LOCUS BBa_K1799019 4139 bp DNA linear 22-Nov-2024 DEFINITION AHL-induced LacZ Generator ACCESSION BBa_K1799019 VERSION BBa_K1799019.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 misc_feature 1..862 /label=BBa_J37019 misc_feature 875..878 /label=conserved RBS 871..882 /label=BBa_B0034 misc_feature 3912..3912 /label=C->T CDS 889..3963 /label=lacZ misc_feature 889..891 /label=START misc_feature 3961..3963 /label=STOP CDS 889..3963 /label=BBa_I732005 misc_feature 871..3963 /label=BBa_I732017 stem_loop 3983..4026 /label=stem_loop misc_feature 3972..4051 /label=BBa_B0010 terminator 3972..4051 /label=BBa_B0010 stem_loop 4071..4114 /label=stem_loop misc_feature 4060..4139 /label=BBa_B0010 terminator 4060..4139 /label=BBa_B0010 terminator 3972..4139 /label=BBa_B0017 CDS 871..4139 /label=BBa_K909006 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag aaagaggaga aatactagat gaccatgatt 901 acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 961 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 1021 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt 1081 ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact 1141 gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta caccaacgtg 1201 acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac gggttgttac 1261 tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt 1321 gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag 1381 gacagtcgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg agaaaaccgc 1441 ctcgcggtga tggtgctgcg ctggagtgac ggcagttatc tggaagatca ggatatgtgg 1501 cggatgagcg gcattttccg tgacgtctcg ttgctgcata aaccgactac acaaatcagc 1561 gatttccatg ttgccactcg ctttaatgat gatttcagcc gcgctgtact ggaggctgaa 1621 gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt atggcagggt 1681 gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg aaattatcga tgagcgtggt 1741 ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa acccgaaact gtggagcgcc 1801 gaaatcccga atctctatcg tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt 1861 gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg 1921 ctgaacggca agccgttgct gattcgaggc gttaaccgtc acgagcatca tcctctgcat 1981 ggtcaggtca tggatgagca gacgatggtg caggatatcc tgctgatgaa gcagaacaac 2041 tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac gctgtgcgac 2101 cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat ggtgccaatg 2161 aatcgtctga ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg 2221 gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa tgaatcaggc 2281 cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg 2341 gtgcagtatg aaggcggcgg agccgacacc acggccaccg atattatttg cccgatgtac 2401 gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga aatggtccat caaaaaatgg 2461 ctttcgctac ctggagagac gcgcccgctg atcctttgcg aatacgccca cgcgatgggt 2521 aacagtcttg gcggtttcgc taaatactgg caggcgtttc gtcagtatcc ccgtttacag 2581 ggcggcttcg tctgggactg ggtggatcag tcgctgatta aatatgatga aaacggcaac 2641 ccgtggtcgg cttacggcgg tgattttggc gatacgccga acgatcgcca gttctgtatg 2701 aacggtctgg tctttgccga ccgcacgccg catccagcgc tgacggaagc aaaacaccag 2761 cagcagtttt tccagttccg tttatccggg caaaccatcg aagtgaccag cgaatacctg 2821 ttccgtcata gcgataacga gctcctgcac tggatggtgg cgctggatgg taagccgctg 2881 gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta aacagttgat tgaactgcct 2941 gaactaccgc agccggagag cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg 3001 aacgcgaccg catggtcaga agccgggcac atcagcgcct ggcagcagtg gcgtctggcg 3061 gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct gaccaccagc 3121 gaaatggatt tttgcatcga gctgggtaat aagcgttggc aatttaaccg ccagtcaggc 3181 tttctttcac agatgtggat tggcgataaa aaacaactgc tgacgccgct gcgcgatcag 3241 ttcacccgtg caccgctgga taacgacatt ggcgtaagtg aagcgacccg cattgaccct 3301 aacgcctggg tcgaacgctg gaaggcggcg ggccattacc aggccgaagc agcgttgttg 3361 cagtgcacgg cagatacact tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag 3421 catcagggga aaaccttatt tatcagccgg aaaacctacc ggattgatgg tagtggtcaa 3481 atggcgatta ccgttgatgt tgaagtggcg agcgatacac cgcatccggc gcggattggc 3541 ctgaactgcc agctggcgca ggtagcagag cgggtaaact ggctcggatt agggccgcaa 3601 gaaaactatc ccgaccgcct tactgccgcc tgttttgacc gctgggatct gccattgtca 3661 gacatgtata ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa 3721 ttgaattatg gcccacacca gtggcgcggc gacttccagt tcaacatcag ccgctacagt 3781 caacagcaac tgatggaaac cagccatcgc catctgctgc acgcggaaga aggcacatgg 3841 ctgaatatcg acggtttcca tatggggatt ggtggcgacg actcctggag cccgtcagta 3901 tcggcggaat ttcagctgag cgccggtcgc taccattacc agttggtctg gtgtcaaaaa 3961 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 4021 ttttatctgt tgtttgtcgg tgaacgctct ctactagagc caggcatcaa ataaaacgaa 4081 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctc //