BBa_K1799000 1 BBa_K1799000 pLsrA-2 2015-08-05T11:00:00Z 2015-09-18T01:13:21Z This part was synthesized from E. coli genomic data retrieved via EcoGene. It is based on, but not identical to, a part created by the 2011 Tokyo Tech iGEM team: http://parts.igem.org/Part:BBa_K649100 Promoter for the E. coli LsrACDB operon. This promoter is repressed by LsrR, and derepressed by autoinducer 2 (AI-2) in the presence of LsrK. false false _2224_ 9151 19613 9 true Promoter begins with the start of its cAMP-CRP binding site and ends after the second key LsrR binding sequence. false Miranda Halle annotation2434487 1 LsrR binding site range2434487 1 94 102 annotation2434485 1 cAMP-CRP binding site range2434485 1 1 21 annotation2434486 1 LsrR binding site range2434486 1 81 86 BBa_K1799026 1 BBa_K1799026 Discosoma RFP with stp 2015-09-17T11:00:00Z 2016-01-21T02:18:57Z BBa_E1010 BBa_E1010 Discosoma RFP with stp false false _2224_ 4206 9151 9 false BBa_E1010 Discosoma RFP with stp false Ellen Jorgensen BBa_K1799027 1 BBa_K1799027 Lac promoter 2015-09-17T11:00:00Z 2015-09-18T03:06:28Z derived from ???lac1??? [Cox et al 2007] derived from ???lac1??? [Cox et al 2007] false false _2224_ 9151 9151 9 false none false Ellen Jorgensen BBa_K1799022 1 BBa_K1799022 IPTG-Repressible RFP Generator 2015-09-16T11:00:00Z 2015-09-18T05:24:56Z pLsrA and LsrR were synthesized by IDT using sequence data provided by EcoGene and converted into RDP format following Synbiota's RDP part creation protocol. All other parts were provided inside Synbiota's RDP assembly kit. Device was designed and assembled using Synbiota's GENtle software and Rapid DNA Prototyping (RDP) system. The device consists of two parts, separated by a terminator. Part 1: LsrR open reading frame, under the control of the Lac promoter and a medium RBS. Part 2: RFP under the control of pLsrA and a medium RBS. When cultured in a LacI- strain or in the presence of IPTG, LsrR is produced, repressing pLsrA and preventing expression of RFP. false false _2224_ 26720 26954 9 true N/A false Will Shindel, Michael Flanagan component2474129 1 BBa_K1799025 component2474121 1 BBa_K1799002 component2474120 1 BBa_K1799028 component2474126 1 BBa_K1799000 component2474122 1 BBa_K1799025 component2474127 1 BBa_K1799028 component2474128 1 BBa_K1799026 component2474119 1 BBa_K1799027 annotation2474120 1 BBa_K1799028 range2474120 1 67 116 annotation2474121 1 BBa_K1799002 range2474121 1 123 1076 annotation2474127 1 BBa_K1799028 range2474127 1 1293 1342 annotation2474119 1 BBa_K1799027 range2474119 1 1 58 annotation2474126 1 BBa_K1799000 range2474126 1 1183 1284 annotation2474128 1 BBa_K1799026 range2474128 1 1351 2025 annotation2474129 1 BBa_K1799025 range2474129 1 2034 2123 annotation2474122 1 BBa_K1799025 range2474122 1 1085 1174 BBa_K1799025 1 BBa_K1799025 Trp attenuator + stop codon 2015-09-17T11:00:00Z 2015-09-18T02:59:43Z Designed by Mike Ellison from Synbiots Terminator and stop codon false false _2224_ 9151 9151 9 false none false Ellen Jorgensen BBa_K1799002 1 BBa_K1799002 LsrR 2015-08-06T11:00:00Z 2015-09-18T01:12:11Z This part was synthesized from E. coli genomic data retrieved via EcoGene: http://www.ecogene.org/gene/EG13805 Gene for the repressor LsrR, present in the E. coli LsrRK operon. Represses the pLsrA promoter, as well as its own promoter, pLsrR false false _2224_ 9151 19613 9 true None. false Miranda Halle BBa_K1799028 1 BBa_K1799028 RBS.2 2015-09-17T11:00:00Z 2015-09-18T03:10:39Z BBa_B0032 Part of the Synbiota parts kit. note BBa_B0032 with appended 5???stp codon + Kpn1 site false false _2224_ 9151 9151 9 false appended 5???stp codon + Kpn1 site false Ellen Jorgensen BBa_K1799027_sequence 1 ttgacattgtgagcggataacaagatactaactgtaacactcgcctattgttctatga BBa_K1799025_sequence 1 tgatgagggtcgggcggattactcgcccgaaaaaaaacttgttttactactcccagcccgcctaatgagcgggcttttttttgaacaaaa BBa_K1799026_sequence 1 gcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggcacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggcaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcctga BBa_K1799002_sequence 1 atgacaatcaacgattcggcaatttcagaacagggaatgtgtgaagaagaacaggtcgcgcggatcgcgtggttttactatcacgacgggctgacccagagcgagatcagcgatcgtctcggcctgacacgtttgaaagtgtcgcgattgctggagaaagggcatcagtccggcattattcgcgtacagattaattctcgctttgaaggctgtctggaatatgaaactcaattacgtcgtcagttttcgctgcaacatgtccgggtgatccctgggcttgcggatgctgatgtcggtgggcgactggggataggcgcggcgcatatgttgatgagtttacttcaaccacaacagatgctggcgattggttttggcgaggcaaccatgaatacgctgcaacgcttaagtggttttatttcgtcacagcaaattcgcctggtcacgctctccggtggcgtcggttcttatatgacgggaatcgggcagcttaacgcggcgtgcagtgtgaatattattccggctccgttgcgggcatcctccgctgacattgcccgtacgctaaaaaatgaaaattgcgtcaaagatgttctgttagccgcgcaagcagcggatgtggcgattgtcggcattggtgctgtgagtcaacaggacgatgcgacaatcattcgctccggttatatcagccagggcgaacagttaatgattggccgaaaaggggcggttggcgacattttaggctacttttttgatgcaaaaggtgacgttgtcacgaatatcaaaatacataacgaactgattggcttacctttaagcgcgctgaagaccatacccgtccgggttggcgtggcagggggagaaaataaagccgaagcaattgccgctgcaatgaaaggcggttatatcaacgcactggttaccgatcaggacacagcagcggcgattttacgtagttaa BBa_K1799000_sequence 1 aattgtgatctattcgtcggaaatatgtgcaatgtccacctaaggttatgaacaaattaaaagcagaaatacatttgttcaaaactcacctgcaaaactgaa BBa_K1799028_sequence 1 tgagtacctcacacaggaaagtctaactcatggagtgtgtcctttcagat BBa_K1799022_sequence 1 ttgacattgtgagcggataacaagatactaactgtaacactcgcctattgttctatgatactagagtgagtacctcacacaggaaagtctaactcatggagtgtgtcctttcagattactagatgacaatcaacgattcggcaatttcagaacagggaatgtgtgaagaagaacaggtcgcgcggatcgcgtggttttactatcacgacgggctgacccagagcgagatcagcgatcgtctcggcctgacacgtttgaaagtgtcgcgattgctggagaaagggcatcagtccggcattattcgcgtacagattaattctcgctttgaaggctgtctggaatatgaaactcaattacgtcgtcagttttcgctgcaacatgtccgggtgatccctgggcttgcggatgctgatgtcggtgggcgactggggataggcgcggcgcatatgttgatgagtttacttcaaccacaacagatgctggcgattggttttggcgaggcaaccatgaatacgctgcaacgcttaagtggttttatttcgtcacagcaaattcgcctggtcacgctctccggtggcgtcggttcttatatgacgggaatcgggcagcttaacgcggcgtgcagtgtgaatattattccggctccgttgcgggcatcctccgctgacattgcccgtacgctaaaaaatgaaaattgcgtcaaagatgttctgttagccgcgcaagcagcggatgtggcgattgtcggcattggtgctgtgagtcaacaggacgatgcgacaatcattcgctccggttatatcagccagggcgaacagttaatgattggccgaaaaggggcggttggcgacattttaggctacttttttgatgcaaaaggtgacgttgtcacgaatatcaaaatacataacgaactgattggcttacctttaagcgcgctgaagaccatacccgtccgggttggcgtggcagggggagaaaataaagccgaagcaattgccgctgcaatgaaaggcggttatatcaacgcactggttaccgatcaggacacagcagcggcgattttacgtagttaatactagagtgatgagggtcgggcggattactcgcccgaaaaaaaacttgttttactactcccagcccgcctaatgagcgggcttttttttgaacaaaatactagagaattgtgatctattcgtcggaaatatgtgcaatgtccacctaaggttatgaacaaattaaaagcagaaatacatttgttcaaaactcacctgcaaaactgaatactagagtgagtacctcacacaggaaagtctaactcatggagtgtgtcctttcagattactagaggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggcacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggcaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcctgatactagagtgatgagggtcgggcggattactcgcccgaaaaaaaacttgttttactactcccagcccgcctaatgagcgggcttttttttgaacaaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z