LOCUS       BBa_K180007             2195 bp    DNA     linear       22-Dec-2024
DEFINITION  Game of Life - Secondary plasmid [tac pR, LuxI generator]
ACCESSION   BBa_K180007
VERSION     BBa_K180007.1  
FEATURES             Location/Qualifiers
     CDS             1..1082
                     /label=lacIq
     promoter        1325..1352
                     /label=Ptac
     promoter        1..1447
                     /label=BBa_K180000
     misc_feature    1460..1463
                     /label=conserved
     RBS             1456..1467
                     /label=BBa_B0034
     CDS             1474..2058
                     /label=LuxI
     CDS             1474..2058
                     /label=BBa_C0161
     stem_loop       2078..2121
                     /label=stem_loop
     misc_feature    2067..2146
                     /label=BBa_B0010
     terminator      2067..2146
                     /label=BBa_B0010
     stem_loop       2162..2181
                     /label=T7 TE
     misc_feature    2188..2188
                     /label=stop
     polyA_site      2182..2195
                     /label=polya
     misc_feature    2155..2195
                     /label=BBa_B0012
     terminator      2155..2195
                     /label=BBa_B0012
ORIGIN
        1 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
       61 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
      121 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
      181 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
      241 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
      301 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
      361 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
      421 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
      481 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
      541 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
      601 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
      661 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
      721 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
      781 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
      841 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
      901 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
      961 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
     1021 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     1081 tgagcgcaac gcaattaatg taagttagct cactcattag gcacaattct catgtttgac
     1141 agcttatcat cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc atcggaagct
     1201 gtggtatggc tgtgcaggtc gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc
     1261 cgttctggat aatgtttttt gcgccgacat cataacggtt ctggcaaata ttctgaaatg
     1321 agctgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg gataacaatt
     1381 tcacacagga aacagccagt ccgtttaggt gttttcacga gcacttcacc aacaaggacc
     1441 atagcattac tagagaaaga ggagaaatac tagatgacta taatgataaa aaaatcggat
     1501 tttttggcaa ttccatcgga ggagtataaa ggtattctaa gtcttcgtta tcaagtgttt
     1561 aagcaaagac ttgagtggga cttagttgta gaaaataacc ttgaatcaga tgagtatgat
     1621 aactcaaatg cagaatatat ttatgcttgt gatgatactg aaaatgtaag tggatgctgg
     1681 cgtttattac ctacaacagg tgattatatg ctgaaaagtg tttttcctga attgcttggt
     1741 caacagagtg ctcccaaaga tcctaatata gtcgaattaa gtcgttttgc tgtaggtaaa
     1801 aatagctcaa agataaataa ctctgctagt gaaattacaa tgaaactatt tgaagctata
     1861 tataaacacg ctgttagtca aggtattaca gaatatgtaa cagtaacatc aacagcaata
     1921 gagcgatttt taaagcgtat taaagttcct tgtcatcgta ttggagacaa agaaattcat
     1981 gtattaggtg atactaaatc ggttgtattg tctatgccta ttaatgaaca gtttaaaaaa
     2041 gcagtcttaa attaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     2101 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     2161 tggctcacct tcgggtgggc ctttctgcgt ttata
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