LOCUS BBa_K180010 3888 bp DNA linear 22-Dec-2024 DEFINITION Rock-paper-scissors - Rock primary plasmid ACCESSION BBa_K180010 VERSION BBa_K180010.1 FEATURES Location/Qualifiers misc_feature 1..927 /label=K180011 misc_feature 936..3888 /label=K180012 misc_feature 1..129 /label=B0015 misc_feature 138..866 /label=C0171 misc_feature 2391..2402 /label=B0034 misc_feature 3125..3136 /label=B0034 misc_feature 2395..2398 /label=conserved misc_feature 3129..3132 /label=conserved misc_feature 873..884 /label=B0034 misc_feature 877..880 /label=conserved misc_feature 893..927 /label=J23119 promoter 922..927 /label=-35 promoter 899..904 /label=-10 misc_feature 936..2382 /label=K180000 CDS 936..2017 /label=lacIq promoter 2260..2287 /label=Ptac misc_feature 2409..3116 /label=K180008 CDS 2409..3116 /label=mCherry CDS 138..866 /label=rhlR misc_feature 3143..3751 /label=C0178 CDS 3143..3751 /label=lasI misc_feature 3760..3888 /label=B0015 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtatta ttagatgaga cccagcgccg cggcgtaggc ggcagccagc 181 gtcttgttcg gcgcgtcgaa cttcttctgg atgttcttgt ggtggaagtt caccgtgctc 241 tcggagatgc tcaggatgat ggcgatttcc ccggaactct tgccgtcggc ggtccattgc 301 aggatctcgc gttcgcgatg gctcaggcag accgggttgg acatcagcat cggatgctcc 361 aggtcggtca gcttctgggt cagcaactcg atcatgcaac gcagccgcag gcggatttcc 421 tcgcgctcga agctggagat gttctgctgg tcgcgcgcca cggaaagcac gctgagcaaa 481 ttgttcggcg cgcggatcgg caaggtcgcg ccgacacaga ggccccaatc gcgagcctcg 541 ttccagagca tccggctctg gtcgaacagg ctgtcgctcc agaccaccat ttccgaggag 601 cgcaggccgt tgaggatcgc cggatccacg gccccgtagt tctgcatctg gtatcgctcc 661 agccaggcct tgggataggt gccatggacc tcggtcttcg gccgggtgaa gggaatcgtg 721 tggcgcacgc cataggcgta gtaatcgaag cccaggcgcc gcacttcctt ttccaggacg 781 gcgaacacgc cctggctgtc gtggatcggc tgcatctcgc tacgcaaacc gtcccaccac 841 agcaaaaagc ctccgtcatt cctcatctag tatttctcct ctttctctag tagctagcat 901 tatacctagg actgagctag ctgtcaatac tagaggtgaa accagtaacg ttatacgatg 961 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc 1021 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc 1081 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct 1141 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc 1201 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag 1261 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg 1321 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg 1381 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac 1441 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat 1501 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc 1561 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa 1621 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga 1681 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct 1741 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca 1801 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc 1861 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca 1921 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 1981 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt 2041 tagctcactc attaggcaca attctcatgt ttgacagctt atcatcgact gcacggtgca 2101 ccaatgcttc tggcgtcagg cagccatcgg aagctgtggt atggctgtgc aggtcgtaaa 2161 tcactgcata attcgtgtcg ctcaaggcgc actcccgttc tggataatgt tttttgcgcc 2221 gacatcataa cggttctggc aaatattctg aaatgagctg ttgacaatta atcatcggct 2281 cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag ccagtccgtt 2341 taggtgtttt cacgagcact tcaccaacaa ggaccatagc attactagag aaagaggaga 2401 aatactagat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc 2461 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg 2521 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc 2581 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg 2641 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt 2701 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc 2761 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg 2821 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg 2881 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact 2941 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct 3001 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac 3061 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtact 3121 agagaaagag gagaaatact agatgatcgt tcagatcggt cgtcgtgaag agttcgacaa 3181 aaaactgctg ggtgaaatgc acaaactgcg tgctcaggtt ttcaaagaac gtaaaggttg 3241 ggacgtttcc gttatcgacg aaatggaaat cgacggttac gacgctctgt ccccgtacta 3301 catgctgatc caggaagaca ccccggaagc tcaggttttc ggttgctggc gtatcttcga 3361 caccaccggt ccgtacatgc tgaaaaacac cttcccggaa ctgctgcacg gtaaagaagc 3421 tccgtgctcc ccgcacatct gggaactgtc ccgtttcgct atcaactccg gtcagaaagg 3481 ttccctgggt ttctccgact gcaccctgga agctatgcgt gctctggctc gttactcctt 3541 gcagaacgac atccagaccc tggttaccgt taccaccgtt ggtgttgaaa aaatgatgat 3601 ccgtgctggt ctggacgttt cccgtttcgg tccgcacctg aaaatcggta tcgaacgtgc 3661 tgttgctctg cgtatcgaac tgaacgctaa aacccagatc gctctgtacg gtggtgttct 3721 ggttgaacag cgtctggctg tttcctaata atactagagc caggcatcaa ataaaacgaa 3781 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 3841 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //