LOCUS       BBa_K180010             3888 bp    DNA     linear       22-Nov-2024
DEFINITION  Rock-paper-scissors - Rock primary plasmid
ACCESSION   BBa_K180010
VERSION     BBa_K180010.1  
FEATURES             Location/Qualifiers
     misc_feature    1..927
                     /label=K180011
     misc_feature    936..3888
                     /label=K180012
     misc_feature    1..129
                     /label=B0015
     misc_feature    138..866
                     /label=C0171
     misc_feature    2391..2402
                     /label=B0034
     misc_feature    3125..3136
                     /label=B0034
     misc_feature    2395..2398
                     /label=conserved
     misc_feature    3129..3132
                     /label=conserved
     misc_feature    873..884
                     /label=B0034
     misc_feature    877..880
                     /label=conserved
     misc_feature    893..927
                     /label=J23119
     promoter        922..927
                     /label=-35
     promoter        899..904
                     /label=-10
     misc_feature    936..2382
                     /label=K180000
     CDS             936..2017
                     /label=lacIq
     promoter        2260..2287
                     /label=Ptac
     misc_feature    2409..3116
                     /label=K180008
     CDS             2409..3116
                     /label=mCherry
     CDS             138..866
                     /label=rhlR
     misc_feature    3143..3751
                     /label=C0178
     CDS             3143..3751
                     /label=lasI
     misc_feature    3760..3888
                     /label=B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggc tctagtatta ttagatgaga cccagcgccg cggcgtaggc ggcagccagc
      181 gtcttgttcg gcgcgtcgaa cttcttctgg atgttcttgt ggtggaagtt caccgtgctc
      241 tcggagatgc tcaggatgat ggcgatttcc ccggaactct tgccgtcggc ggtccattgc
      301 aggatctcgc gttcgcgatg gctcaggcag accgggttgg acatcagcat cggatgctcc
      361 aggtcggtca gcttctgggt cagcaactcg atcatgcaac gcagccgcag gcggatttcc
      421 tcgcgctcga agctggagat gttctgctgg tcgcgcgcca cggaaagcac gctgagcaaa
      481 ttgttcggcg cgcggatcgg caaggtcgcg ccgacacaga ggccccaatc gcgagcctcg
      541 ttccagagca tccggctctg gtcgaacagg ctgtcgctcc agaccaccat ttccgaggag
      601 cgcaggccgt tgaggatcgc cggatccacg gccccgtagt tctgcatctg gtatcgctcc
      661 agccaggcct tgggataggt gccatggacc tcggtcttcg gccgggtgaa gggaatcgtg
      721 tggcgcacgc cataggcgta gtaatcgaag cccaggcgcc gcacttcctt ttccaggacg
      781 gcgaacacgc cctggctgtc gtggatcggc tgcatctcgc tacgcaaacc gtcccaccac
      841 agcaaaaagc ctccgtcatt cctcatctag tatttctcct ctttctctag tagctagcat
      901 tatacctagg actgagctag ctgtcaatac tagaggtgaa accagtaacg ttatacgatg
      961 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc
     1021 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc
     1081 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct
     1141 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc
     1201 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag
     1261 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg
     1321 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg
     1381 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac
     1441 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat
     1501 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc
     1561 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa
     1621 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga
     1681 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct
     1741 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca
     1801 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc
     1861 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca
     1921 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     1981 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt
     2041 tagctcactc attaggcaca attctcatgt ttgacagctt atcatcgact gcacggtgca
     2101 ccaatgcttc tggcgtcagg cagccatcgg aagctgtggt atggctgtgc aggtcgtaaa
     2161 tcactgcata attcgtgtcg ctcaaggcgc actcccgttc tggataatgt tttttgcgcc
     2221 gacatcataa cggttctggc aaatattctg aaatgagctg ttgacaatta atcatcggct
     2281 cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag ccagtccgtt
     2341 taggtgtttt cacgagcact tcaccaacaa ggaccatagc attactagag aaagaggaga
     2401 aatactagat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     2461 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     2521 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     2581 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     2641 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     2701 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     2761 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     2821 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     2881 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     2941 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     3001 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     3061 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtact
     3121 agagaaagag gagaaatact agatgatcgt tcagatcggt cgtcgtgaag agttcgacaa
     3181 aaaactgctg ggtgaaatgc acaaactgcg tgctcaggtt ttcaaagaac gtaaaggttg
     3241 ggacgtttcc gttatcgacg aaatggaaat cgacggttac gacgctctgt ccccgtacta
     3301 catgctgatc caggaagaca ccccggaagc tcaggttttc ggttgctggc gtatcttcga
     3361 caccaccggt ccgtacatgc tgaaaaacac cttcccggaa ctgctgcacg gtaaagaagc
     3421 tccgtgctcc ccgcacatct gggaactgtc ccgtttcgct atcaactccg gtcagaaagg
     3481 ttccctgggt ttctccgact gcaccctgga agctatgcgt gctctggctc gttactcctt
     3541 gcagaacgac atccagaccc tggttaccgt taccaccgtt ggtgttgaaa aaatgatgat
     3601 ccgtgctggt ctggacgttt cccgtttcgg tccgcacctg aaaatcggta tcgaacgtgc
     3661 tgttgctctg cgtatcgaac tgaacgctaa aacccagatc gctctgtacg gtggtgttct
     3721 ggttgaacag cgtctggctg tttcctaata atactagagc caggcatcaa ataaaacgaa
     3781 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     3841 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//