LOCUS       BBa_K180012             2953 bp    DNA     linear       22-Nov-2024
DEFINITION  Rock - Primary plasmid (part 2) [tac pR, mCherry and LasI 
            generator]
ACCESSION   BBa_K180012
VERSION     BBa_K180012.1  
FEATURES             Location/Qualifiers
     CDS             1..1082
                     /label=lacIq
     promoter        1325..1352
                     /label=Ptac
     promoter        1..1447
                     /label=BBa_K180000
     misc_feature    1460..1463
                     /label=conserved
     RBS             1456..1467
                     /label=BBa_B0034
     CDS             1474..2181
                     /label=mCherry
     CDS             1474..2181
                     /label=BBa_K180008
     misc_feature    2194..2197
                     /label=conserved
     RBS             2190..2201
                     /label=BBa_B0034
     CDS             2208..2816
                     /label=lasI
     CDS             2208..2816
                     /label=BBa_C0178
     stem_loop       2836..2879
                     /label=stem_loop
     misc_feature    2825..2904
                     /label=BBa_B0010
     terminator      2825..2904
                     /label=BBa_B0010
     stem_loop       2920..2939
                     /label=T7 TE
     misc_feature    2946..2946
                     /label=stop
     polyA_site      2940..2953
                     /label=polya
     misc_feature    2913..2953
                     /label=BBa_B0012
     terminator      2913..2953
                     /label=BBa_B0012
ORIGIN
        1 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
       61 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
      121 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
      181 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
      241 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
      301 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
      361 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
      421 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
      481 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
      541 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
      601 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
      661 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
      721 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
      781 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
      841 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
      901 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
      961 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
     1021 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     1081 tgagcgcaac gcaattaatg taagttagct cactcattag gcacaattct catgtttgac
     1141 agcttatcat cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc atcggaagct
     1201 gtggtatggc tgtgcaggtc gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc
     1261 cgttctggat aatgtttttt gcgccgacat cataacggtt ctggcaaata ttctgaaatg
     1321 agctgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg gataacaatt
     1381 tcacacagga aacagccagt ccgtttaggt gttttcacga gcacttcacc aacaaggacc
     1441 atagcattac tagagaaaga ggagaaatac tagatggtga gcaagggcga ggaggataac
     1501 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     1561 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     1621 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     1681 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     1741 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     1801 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     1861 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     1921 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     1981 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     2041 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     2101 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     2161 ggcatggacg agctgtacaa gtactagaga aagaggagaa atactagatg atcgttcaga
     2221 tcggtcgtcg tgaagagttc gacaaaaaac tgctgggtga aatgcacaaa ctgcgtgctc
     2281 aggttttcaa agaacgtaaa ggttgggacg tttccgttat cgacgaaatg gaaatcgacg
     2341 gttacgacgc tctgtccccg tactacatgc tgatccagga agacaccccg gaagctcagg
     2401 ttttcggttg ctggcgtatc ttcgacacca ccggtccgta catgctgaaa aacaccttcc
     2461 cggaactgct gcacggtaaa gaagctccgt gctccccgca catctgggaa ctgtcccgtt
     2521 tcgctatcaa ctccggtcag aaaggttccc tgggtttctc cgactgcacc ctggaagcta
     2581 tgcgtgctct ggctcgttac tccttgcaga acgacatcca gaccctggtt accgttacca
     2641 ccgttggtgt tgaaaaaatg atgatccgtg ctggtctgga cgtttcccgt ttcggtccgc
     2701 acctgaaaat cggtatcgaa cgtgctgttg ctctgcgtat cgaactgaac gctaaaaccc
     2761 agatcgctct gtacggtggt gttctggttg aacagcgtct ggctgtttcc taataatact
     2821 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     2881 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     2941 ttctgcgttt ata
//