LOCUS       BBa_K180013             1369 bp    DNA     linear       26-Dec-2024
DEFINITION  Rock-paper-scissors - Rock secondary plasmid [rhl pR, LacI 
            generator]
ACCESSION   BBa_K180013
VERSION     BBa_K180013.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=RhlR
     promoter        19..24
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     promoter        1..53
                     /label=BBa_R0071
     misc_feature    66..69
                     /label=conserved
     RBS             62..73
                     /label=BBa_B0034
     misc_feature    1169..1207
                     /label=LVA
     CDS             80..1207
                     /label=lacI-LVA
     misc_feature    80..1207
                     /label=BBa_C0012
     misc_feature    1208..1232
                     /label=Help:Barcodes
     CDS             80..1207
                     /label=BBa_C0012
     stem_loop       1252..1295
                     /label=stem_loop
     misc_feature    1241..1320
                     /label=BBa_B0010
     terminator      1241..1320
                     /label=BBa_B0010
     stem_loop       1336..1355
                     /label=T7 TE
     misc_feature    1362..1362
                     /label=stop
     polyA_site      1356..1369
                     /label=polya
     misc_feature    1329..1369
                     /label=BBa_B0012
     terminator      1329..1369
                     /label=BBa_B0012
     terminator      1241..1369
                     /label=BBa_B0015
ORIGIN
        1 tcctgtgaaa tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttctactaga
       61 gaaagaggag aaatactaga tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
      121 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
      181 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
      241 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
      301 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
      361 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
      421 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
      481 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
      541 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt
      601 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
      661 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
      721 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
      781 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
      841 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
      901 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca
      961 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
     1021 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
     1081 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     1141 acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc
     1201 ttaataactc tgatagtgct agtgtagatc tctactagag ccaggcatca aataaaacga
     1261 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1321 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//