LOCUS BBa_K180014 3952 bp DNA linear 22-Nov-2024 DEFINITION Rock-paper-scissors - Paper primary plasmid ACCESSION BBa_K180014 VERSION BBa_K180014.1 FEATURES Location/Qualifiers misc_feature 1..979 /label=K180005 misc_feature 988..3952 /label=K180015 misc_feature 1..129 /label=B0015 misc_feature 163..918 /label=C0062 CDS 169..918 /label=luxR misc_feature 2443..2454 /label=B0034 misc_feature 2447..2450 /label=conserved misc_feature 3189..3200 /label=B0034 misc_feature 3193..3196 /label=conserved misc_feature 925..936 /label=B0034 misc_feature 929..932 /label=conserved misc_feature 945..979 /label=J23119 promoter 975..979 /label=-35 promoter 951..956 /label=-10 misc_feature 988..2434 /label=K180000 CDS 988..2069 /label=lacIq promoter 2312..2339 /label=Ptac misc_feature 2461..3180 /label=E0040 CDS 2461..3180 /label=GFPmut3b misc_feature 3207..3815 /label=C0170 CDS 3207..3815 /label=rhlI misc_feature 3824..3952 /label=B0015 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaat ttttaaagta 181 tgggcaatca attgctcctg ttaaaattgc tttagaaata ctttggcagc ggtttgttgt 241 attgagtttc atttgcgcat tggttaaatg gaaagtgaca gtacgctcac tgcaacctaa 301 tatttttgaa atatcccaag agctttttcc ttcgcatgcc cacgctaaac attctttttc 361 tcttttggtt aaatcgttgt ttgatttatt atttgctata tttatttttc gataattatc 421 aactagagaa ggaacaatta atggtatgtt catacacgca tgtaaaaata aactatctat 481 atagttgtct ttttctgaat gtgcaaaact aagcattccg aagccattgt tagccgtatg 541 aatagggaaa ctaaacccag tgataagacc tgatgttttc gcttctttaa ttacatttgg 601 agatttttta tttacagcat tgttttcaaa tatattccaa ttaattggtg aatgattgga 661 gttagaataa tctactatag gatcatattt tattaaatta gcgtcatcat aatattgcct 721 ccatttttta gggtaattat ctaggattga aatatcagat ttaaccatag aatgaggata 781 aatgatcgcg agtaaataat attcacaatg taccatttta gtcatatcag ataagcattg 841 attaatatca ttattgcttc tacaagcttt aattttatta attattctgt atgtgtcgtc 901 ggcatttatg tttttcatct agtatttctc ctctttctct agtagctagc attataccta 961 ggactgagct agctgtcaat actagaggtg aaaccagtaa cgttatacga tgtcgcagag 1021 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct 1081 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc 1141 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg 1201 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt 1261 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg 1321 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag 1381 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct 1441 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg 1501 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct 1561 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag 1621 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa 1681 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg 1741 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg 1801 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag 1861 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag 1921 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg 1981 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 2041 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtaa gttagctcac 2101 tcattaggca caattctcat gtttgacagc ttatcatcga ctgcacggtg caccaatgct 2161 tctggcgtca ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta aatcactgca 2221 taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg ccgacatcat 2281 aacggttctg gcaaatattc tgaaatgagc tgttgacaat taatcatcgg ctcgtataat 2341 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agccagtccg tttaggtgtt 2401 ttcacgagca cttcaccaac aaggaccata gcattactag agaaagagga gaaatactag 2461 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt 2521 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga 2581 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt 2641 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag 2701 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc 2761 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt 2821 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa 2881 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga 2941 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac 3001 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac 3061 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt 3121 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa 3181 tactagagaa agaggagaaa tactagatga tcgaactgct gtccgaatcc ctggaaggtc 3241 tgtccgctgc tatgatcgct gaactgggtc gttaccgtca ccaggttttc atcgaaaaac 3301 tgggttggga cgttgtttcc acctcccgtg ttcgtgacca ggagttcgac cagttcgacc 3361 acccgcagac ccgttacatc gttgctatgt cccgtcaggg tatctgcggt tgcgctcgtc 3421 tgctgccgac caccgacgct tacctgctga aagacgtttt cgcttacctg tgctccgaaa 3481 ccccgccgtc cgacccgtcc gtttgggaac tgtcccgtta cgctgcttcc gctgctgacg 3541 acccgcagct ggctatgaaa atcttctggt cctccctcca gtgcgcttgg tacctgggtg 3601 cttcctccgt tgttgctgtt accaccaccg ctatggaacg ttacttcgtt cgtaacggtg 3661 ttatcctcca gcgtctgggt ccgccgcaga aagttaaagg tgaaaccctg gttgctatct 3721 ccttcccggc ttaccaggaa cgtggtctgg aaatgctgct gcgttaccac ccggaatggc 3781 tccagggtgt tccgctgtcc atggctgttt aataatacta gagccaggca tcaaataaaa 3841 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 3901 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //