##gff-version 3 ##sequence-region BBa_K180016 1 1371 BBa_K180016 . terminator 1243 1371 . + 0 ID=BBa_B0015;Name=BBa_B0015 BBa_K180016 . terminator 1243 1322 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K180016 . stem_loop 1254 1297 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K180016 . engineered_region 1243 1322 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K180016 . terminator 1331 1371 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K180016 . polyA_site 1358 1371 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K180016 . stem_loop 1338 1357 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K180016 . engineered_region 1331 1371 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K180016 . stop_codon 1364 1364 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K180016 . ribosome_entry_site 64 75 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_K180016 . conserved_region 68 71 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K180016 . CDS 82 1209 . + 0 ID=BBa_C0012;Name=BBa_C0012 BBa_K180016 . engineered_region 82 1209 . + 0 ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012 BBa_K180016 . CDS 82 1209 . + 0 ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012 BBa_K180016 . engineered_tag 1210 1234 . + 0 ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012 BBa_K180016 . tag 1171 1209 . + 0 ID=annotation1722;Name=LVA;Parent=BBa_C0012 BBa_K180016 . promoter 1 55 . + 0 ID=BBa_R0062;Name=BBa_R0062 BBa_K180016 . promoter 20 25 . + 0 ID=annotation2046;Name=-35;Parent=BBa_R0062 BBa_K180016 . non_covalent_binding_site 1 20 . + 0 ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062 BBa_K180016 . promoter 42 47 . + 0 ID=annotation2047;Name=-10;Parent=BBa_R0062 BBa_K180016 . engineered_region 1 55 . + 0 ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062 BBa_K180016 . start_codon 53 53 . + 0 ID=annotation2048;Name=start;Parent=BBa_R0062 >BBa_K180016 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact agatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgt ggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattccc aaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgc cgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaag cggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctg gatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacac ccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagca aatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgc aatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctga atgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtc cgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgtta accaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcgg tgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctcc ccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaa aactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaa ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca ccttcgggtgggcctttctgcgtttata