LOCUS BBa_K180017 3858 bp DNA linear 22-Nov-2024 DEFINITION Rock-paper-scissors - Scissors primary plasmid ACCESSION BBa_K180017 VERSION BBa_K180017.1 FEATURES Location/Qualifiers misc_feature 1..129 /label=B0015 misc_feature 1..921 /label=K180018 CDS 138..860 /label=lasR misc_feature 138..860 /label=C0179 misc_feature 867..878 /label=B0034 misc_feature 871..874 /label=conserved misc_feature 887..921 /label=J23119 promoter 893..898 /label=-10 promoter 916..921 /label=-35 CDS 930..2011 /label=lacIq misc_feature 930..2376 /label=K180000 misc_feature 930..3858 /label=K180019 promoter 2254..2281 /label=Ptac misc_feature 2385..2396 /label=B0034 misc_feature 2389..2392 /label=conserved misc_feature 2390..2393 /label=conserved misc_feature 2403..3110 /label=K180009 CDS 2403..3110 /label=mBanana misc_feature 3119..3130 /label=B0034 misc_feature 3137..3721 /label=C0161 CDS 3137..3721 /label=lacI misc_feature 3730..3858 /label=B0015 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtatta ttagagagta ataagaccca aattaacggc cataatggcc 181 gctacgcggc gggaggtcac accgaacttc cgccgaatat ttcccatatg gaagttcaca 241 ttggcttccg agcagttgca gataaccgat atctcccaac tggtcttgcc gatggcgcac 301 cactgcaaca cttccttctc ccggctggtc agaaccaccg gtttgctgac cggatgttcg 361 aaggccagtc cggcaccgct ttgcagtgcg tagtccttga gcatccacag ggtcggcagg 421 accgactcta tgaaacggtt ggcctcggcc cggttttccg cttccacgct gaggctcagc 481 gcgccgagtt cgccgcgagc accatgcagc ggcatggtca gcccatacac caggccggcg 541 gccgaggctt cctcgaagaa ctcgtgctgc tttcgcgtct ggtagatgga cggttcccag 601 aaaatcggca gtacgctctg ggtacagtga ctgaccgtcg ggtcgacccg cgcgtagcca 661 gcccggtcgt aatgctcgcg ccaggcggcc gggtagttgc cgacgatgaa ggcgttctcg 721 tagtcctggc tgtccttagg caacaggccg aacaggatct tcgagaatcc aaggtcgctc 781 gccatcttct ggaggatggc gctccactcc aattttccac ttgagcgttc cagctcaaga 841 aaaccgtcaa ccaaggccat ctagtatttc tcctctttct ctagtagcta gcattatacc 901 taggactgag ctagctgtca atactagagg tgaaaccagt aacgttatac gatgtcgcag 961 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 1021 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 1081 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 1141 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 1201 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 1261 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 1321 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 1381 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 1441 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 1501 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 1561 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 1621 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 1681 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 1741 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 1801 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 1861 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 1921 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 1981 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt aagttagctc 2041 actcattagg cacaattctc atgtttgaca gcttatcatc gactgcacgg tgcaccaatg 2101 cttctggcgt caggcagcca tcggaagctg tggtatggct gtgcaggtcg taaatcactg 2161 cataattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc 2221 ataacggttc tggcaaatat tctgaaatga gctgttgaca attaatcatc ggctcgtata 2281 atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagccagtc cgtttaggtg 2341 ttttcacgag cacttcacca acaaggacca tagcattact agagaaagag gagaaatact 2401 agatggtgag caagggcgag gagaataaca tggccgtcat caaggagttc atgcgcttca 2461 aggtgcgcat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg 2521 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc 2581 ccttcgcctg ggacatcctg tcccctcagt tctgttacgg ctccaaggcc tacgtgaagc 2641 accccactgg tatccccgac tacttcaagc tgtccttccc cgagggcttc aagtgggagc 2701 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtggc tcaggactcc tccctgcagg 2761 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg 2821 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg 2881 gcgccctgaa gggcgagatc aagatgaggc tgaagctgaa ggacggcggc cactacagcg 2941 ccgagaccaa gaccacctac aaggccaaga agcccgtgca gttgcccggc gcctacatag 3001 ccggcgagaa gatcgacatc acctcccaca atgaggacta cactatcgtg gaattgtacg 3061 agcgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag tactagagaa 3121 agaggagaaa tactagatga ctataatgat aaaaaaatcg gattttttgg caattccatc 3181 ggaggagtat aaaggtattc taagtcttcg ttatcaagtg tttaagcaaa gacttgagtg 3241 ggacttagtt gtagaaaata accttgaatc agatgagtat gataactcaa atgcagaata 3301 tatttatgct tgtgatgata ctgaaaatgt aagtggatgc tggcgtttat tacctacaac 3361 aggtgattat atgctgaaaa gtgtttttcc tgaattgctt ggtcaacaga gtgctcccaa 3421 agatcctaat atagtcgaat taagtcgttt tgctgtaggt aaaaatagct caaagataaa 3481 taactctgct agtgaaatta caatgaaact atttgaagct atatataaac acgctgttag 3541 tcaaggtatt acagaatatg taacagtaac atcaacagca atagagcgat ttttaaagcg 3601 tattaaagtt ccttgtcatc gtattggaga caaagaaatt catgtattag gtgatactaa 3661 atcggttgta ttgtctatgc ctattaatga acagtttaaa aaagcagtct taaattaata 3721 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 3781 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 3841 ggcctttctg cgtttata //