LOCUS       BBa_K180019             2929 bp    DNA     linear       22-Nov-2024
DEFINITION  Scissors - Primary plasmid (part 2) [tac pR, mBanana and LuxI 
            generator]
ACCESSION   BBa_K180019
VERSION     BBa_K180019.1  
FEATURES             Location/Qualifiers
     CDS             1..1082
                     /label=lacIq
     promoter        1325..1352
                     /label=Ptac
     promoter        1..1447
                     /label=BBa_K180000
     misc_feature    1460..1463
                     /label=conserved
     RBS             1456..1467
                     /label=BBa_B0034
     CDS             1474..2181
                     /label=mBanana
     CDS             1474..2181
                     /label=BBa_K180009
     misc_feature    2194..2197
                     /label=conserved
     RBS             2190..2201
                     /label=BBa_B0034
     CDS             2208..2792
                     /label=LuxI
     CDS             2208..2792
                     /label=BBa_C0161
     stem_loop       2812..2855
                     /label=stem_loop
     misc_feature    2801..2880
                     /label=BBa_B0010
     terminator      2801..2880
                     /label=BBa_B0010
     stem_loop       2896..2915
                     /label=T7 TE
     misc_feature    2922..2922
                     /label=stop
     polyA_site      2916..2929
                     /label=polya
     misc_feature    2889..2929
                     /label=BBa_B0012
     terminator      2889..2929
                     /label=BBa_B0012
ORIGIN
        1 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
       61 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
      121 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
      181 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
      241 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
      301 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
      361 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
      421 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
      481 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
      541 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
      601 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
      661 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
      721 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
      781 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
      841 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
      901 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
      961 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
     1021 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     1081 tgagcgcaac gcaattaatg taagttagct cactcattag gcacaattct catgtttgac
     1141 agcttatcat cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc atcggaagct
     1201 gtggtatggc tgtgcaggtc gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc
     1261 cgttctggat aatgtttttt gcgccgacat cataacggtt ctggcaaata ttctgaaatg
     1321 agctgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg gataacaatt
     1381 tcacacagga aacagccagt ccgtttaggt gttttcacga gcacttcacc aacaaggacc
     1441 atagcattac tagagaaaga ggagaaatac tagatggtga gcaagggcga ggagaataac
     1501 atggccgtca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc
     1561 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     1621 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     1681 ttctgttacg gctccaaggc ctacgtgaag caccccactg gtatccccga ctacttcaag
     1741 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     1801 gtgaccgtgg ctcaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     1861 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     1921 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagatgagg
     1981 ctgaagctga aggacggcgg ccactacagc gccgagacca agaccaccta caaggccaag
     2041 aagcccgtgc agttgcccgg cgcctacata gccggcgaga agatcgacat cacctcccac
     2101 aatgaggact acactatcgt ggaattgtac gagcgcgccg agggccgcca ctccaccggc
     2161 ggcatggacg agctgtacaa gtactagaga aagaggagaa atactagatg actataatga
     2221 taaaaaaatc ggattttttg gcaattccat cggaggagta taaaggtatt ctaagtcttc
     2281 gttatcaagt gtttaagcaa agacttgagt gggacttagt tgtagaaaat aaccttgaat
     2341 cagatgagta tgataactca aatgcagaat atatttatgc ttgtgatgat actgaaaatg
     2401 taagtggatg ctggcgttta ttacctacaa caggtgatta tatgctgaaa agtgtttttc
     2461 ctgaattgct tggtcaacag agtgctccca aagatcctaa tatagtcgaa ttaagtcgtt
     2521 ttgctgtagg taaaaatagc tcaaagataa ataactctgc tagtgaaatt acaatgaaac
     2581 tatttgaagc tatatataaa cacgctgtta gtcaaggtat tacagaatat gtaacagtaa
     2641 catcaacagc aatagagcga tttttaaagc gtattaaagt tccttgtcat cgtattggag
     2701 acaaagaaat tcatgtatta ggtgatacta aatcggttgt attgtctatg cctattaatg
     2761 aacagtttaa aaaagcagtc ttaaattaat aatactagag ccaggcatca aataaaacga
     2821 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2881 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//