LOCUS BBa_K180020 1473 bp DNA linear 31-Oct-2024 DEFINITION Rock-paper-scissors - Scissors secondary plasmid [las pR, LacI generator] ACCESSION BBa_K180020 VERSION BBa_K180020.1 FEATURES Location/Qualifiers misc_feature 46..63 /label=OP2 misc_feature 106..125 /label=OP1 promoter 117..122 /label=-35 promoter 140..145 /label=-10 promoter 1..157 /label=BBa_R0079 misc_feature 170..173 /label=conserved RBS 166..177 /label=BBa_B0034 misc_feature 1273..1311 /label=LVA CDS 184..1311 /label=lacI-LVA misc_feature 184..1311 /label=BBa_C0012 misc_feature 1312..1336 /label=Help:Barcodes CDS 184..1311 /label=BBa_C0012 stem_loop 1356..1399 /label=stem_loop misc_feature 1345..1424 /label=BBa_B0010 terminator 1345..1424 /label=BBa_B0010 stem_loop 1440..1459 /label=T7 TE misc_feature 1466..1466 /label=stop polyA_site 1460..1473 /label=polya misc_feature 1433..1473 /label=BBa_B0012 terminator 1433..1473 /label=BBa_B0012 terminator 1345..1473 /label=BBa_B0015 ORIGIN 1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct 61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg 121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagaaaga ggagaaatac 181 tagatggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct 241 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa 301 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg 361 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg 421 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg 481 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg 541 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg 601 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc 661 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca 721 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg 781 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg 841 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc 901 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc 961 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc 1021 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg 1081 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat 1141 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc 1201 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 1261 gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag 1321 tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 1381 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 1441 gctcaccttc gggtgggcct ttctgcgttt ata //