LOCUS       BBa_K180020             1473 bp    DNA     linear       23-Dec-2024
DEFINITION  Rock-paper-scissors - Scissors secondary plasmid [las pR, LacI 
            generator]
ACCESSION   BBa_K180020
VERSION     BBa_K180020.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=OP2
     misc_feature    106..125
                     /label=OP1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..157
                     /label=BBa_R0079
     misc_feature    170..173
                     /label=conserved
     RBS             166..177
                     /label=BBa_B0034
     misc_feature    1273..1311
                     /label=LVA
     CDS             184..1311
                     /label=lacI-LVA
     misc_feature    184..1311
                     /label=BBa_C0012
     misc_feature    1312..1336
                     /label=Help:Barcodes
     CDS             184..1311
                     /label=BBa_C0012
     stem_loop       1356..1399
                     /label=stem_loop
     misc_feature    1345..1424
                     /label=BBa_B0010
     terminator      1345..1424
                     /label=BBa_B0010
     stem_loop       1440..1459
                     /label=T7 TE
     misc_feature    1466..1466
                     /label=stop
     polyA_site      1460..1473
                     /label=polya
     misc_feature    1433..1473
                     /label=BBa_B0012
     terminator      1433..1473
                     /label=BBa_B0012
     terminator      1345..1473
                     /label=BBa_B0015
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagaaaga ggagaaatac
      181 tagatggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct
      241 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa
      301 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg
      361 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg
      421 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg
      481 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg
      541 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg
      601 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc
      661 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca
      721 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg
      781 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg
      841 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc
      901 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc
      961 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc
     1021 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg
     1081 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat
     1141 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc
     1201 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     1261 gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag
     1321 tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1381 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1441 gctcaccttc gggtgggcct ttctgcgttt ata
//