##gff-version 3
##sequence-region BBa_K180020 1 1473
BBa_K180020	.	ribosome_entry_site	166	177	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K180020	.	conserved_region	170	173	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K180020	.	promoter	1	157	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_K180020	.	non_covalent_binding_site	46	63	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_K180020	.	promoter	140	145	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_K180020	.	promoter	117	122	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_K180020	.	non_covalent_binding_site	106	125	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_K180020	.	terminator	1345	1473	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K180020	.	terminator	1433	1473	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K180020	.	stem_loop	1440	1459	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K180020	.	engineered_region	1433	1473	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K180020	.	stop_codon	1466	1466	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K180020	.	polyA_site	1460	1473	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K180020	.	terminator	1345	1424	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K180020	.	engineered_region	1345	1424	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K180020	.	stem_loop	1356	1399	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K180020	.	CDS	184	1311	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K180020	.	engineered_tag	1312	1336	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K180020	.	CDS	184	1311	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K180020	.	tag	1273	1311	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K180020	.	engineered_region	184	1311	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
>BBa_K180020
gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaaca
tacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagct
actagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtc
tcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcga
tggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccac
ctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtg
gtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtg
ggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatt
tcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcat
ctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctg
gctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttt
tcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggc
gcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagaca
gctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgct
gcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcg
cccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaa
gcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata