BBa_K1806002 1 BBa_K1806002 T7 lacO pelB RFP 6xHis 2015-09-16T11:00:00Z 2015-09-17T07:13:43Z This is a composite part composed of several parts and designed in the light of literary findings. The part has been designed as a backbone part to integrate the T7 lacO pelB + 6xHis system to a targeted sequence. The RFP has BamH1 and Xho1 restriction sites to be cut out and replaced by the targeted sequence. T7 lacO allows the system to be inducible by iPTG and the pelB signal peptide allows the cells to excrete the translated proteins placed in the following sequence. The 6xHis is present for protein analysis. false false _2231_ 11153 11153 9 false none false İBRAHİM YASİR ORHAN BBa_K1806002_sequence 1 gagatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcccctccagaaataattttgtttaactttaagaaggagatatacatatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatggatatcggaattaattcggatccgatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcgtacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcgtccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaactcgagcaccaccatcaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z