LOCUS BBa_K1807007 3934 bp DNA linear 29-Nov-2024 DEFINITION Synthetic Phosphate-specific Transporter Operon ACCESSION BBa_K1807007 VERSION BBa_K1807007.1 FEATURES Location/Qualifiers misc_feature 1..54 /label=BBa_R0011 misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 68..71 /label=conserved misc_feature 64..75 /label=BBa_B0034 CDS 82..1101 /label=PstS RBS 1108..1119 /label=BBa_B0034 CDS 1126..2103 /label=PstC RBS 2101..2112 /label=BBa_B0034 CDS 2128..2994 /label=PstA RBS 3001..3012 /label=BBa_B0034 CDS 3019..3804 /label=PstB misc_feature 82..3804 /label=BBa_K1807013 misc_feature 3813..3892 /label=BBa_B0010 stem_loop 3824..3867 /label=stem_loop misc_feature 3901..3941 /label=BBa_B0012 stem_loop 3908..3927 /label=T7 TE polyA_site 3928..3941 /label=polya misc_feature 3934..3934 /label=stop misc_feature 3813..3941 /label=BBa_B0015 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacga 61 gaaagaggag aaatacccca tgacgaccca tcgcacactg tgtaaattgt ccaccctggc 121 ggcggcgctg agtcttagtc tgagtacgct ggcagctgat atcaccggtg cgggtgccac 181 cttcccgtac gcggtatata ccaaatgggc agaagcgtac cagaccgcga cccgcaaccg 241 tgtgaactat caaggaattg gctcgtctgg tggtgtgaag cagattaaag cgcgtacggt 301 ggattttggt ggtacagatg ccccgctgtc ggagagcgac ctggacgccg cgggcttagt 361 gcaggtgccg accgtcatgg ggggcgtgac attggtcatc aacgtgccgg gagtggaatc 421 cggcaaactg aaactggacg gcccgaccgt agccgatctc ttccgcggtg caatccgtcg 481 ctggaatgat cctgcaatcg tcaatttgaa tccggggtta aagctgccag atctcgcaat 541 tacggtcgcc tttcgcagcg atggctccgg cactacgttt gtcttcagca actaccttgc 601 gcgccaaagc gccagcttta aaaacgaagt gggtgcgggc aataccgtca actggccggc 661 aaatggcgtg ggcggtaaag gtaatccggg cgtagcagca aacgtccaga aaatcgccgg 721 cgcgattgga tacgtagata ttgctgatgc aaccaaaaac aagatgaatt tcgttgcatt 781 gaagaataag ttcggtcact atatcgtccc gactcaggac gctgtggaag ctgccgccgc 841 gggggcaaat tttaaagtaa aaggcatggc gcctgacctg ctggaccagg cgcataaaga 901 tgcatggccg atcaccagtg ccacgtatat cctggcgtac gaaaagggca cggatgcggc 961 taaacaaaaa ggcgttgtag attttttcgg ctgggcgttg aacaacggcc aaaaaatggc 1021 cgaagatttg ggttttgtac cactgcccgc ctcggttgta aaaatggtgg aagcagagat 1081 gaagaacatc aagtaatgat acgagaaaga ggagaaatca agtatgagca ccagccgccc 1141 ggtccgttcg gacctgcaag cgcgtcagat ccgtaaattc aaactccagg ataccctgtt 1201 ccatcagctg acgttattgt tcgctcttct ggtgctcgcc gcgttaacag gcattttgat 1261 tgcgttggct gttgaagcac tgcctgcctt tcgcgaattt ggtgcggctt ttttatggac 1321 gccggtttgg aacgttccgg aagcacagtt cggtgcgctt tcggcgattt acggcacggt 1381 ggtgacgagt gcgatcgccc tgctcattgc cgtcccggtc tcgtttggaa ttgccttgtt 1441 tttgaccgag acgtgtccgg tgtggttacg ccgccctctg ggcacagcta ttgagctctt 1501 agcaggagta ccatctatca tctacgggat ttggggcctg tttgtgtttg ccccgttatt 1561 tgcgcgccac gtccaaccct atctgcaaac ggtgttcggc gatttgccgg taattggcgg 1621 ttggtttgca ggcccgccga ttggcgttgg cattttaacg gccgcaatcg tgctgagcct 1681 tatggtgatc ccttttgttg cgagcgtcat gcgtgacgtt tttgagacgg tgccggcgca 1741 actgaaagaa tcagcgtacg gcctgggttg cactacgtgg gaagtcgtca caggcgtggt 1801 tctgccatac actcgtgttg gcgttatcgg cggtatcatg ttagggctgg ggcgcgcact 1861 tggcgaaacc atggcggtta cctttgttat tgggaacgcg aaccgtattg ttggctcctt 1921 attcgcaccg ggcacttcta tcgcctctac gctggccaat gagttcggtg aggctgaccc 1981 tggactgcac attagtgcat tgtttgcgct cggcctggtc ttgttcgtga tcaccttcgt 2041 ggtactggcg atttcccgct ggctgattca gcgtgggtta gctcgtcagg ggaaataatg 2101 atacgagaaa gaggagaaat caagtatgcg ccgcggccgc ccagttatga atcgttattc 2161 tcgccgtatc ctgaccaacc gcatcggaat tggtcttagt atgctggcga tggcgttcgg 2221 tttactggcc ctgctgtgga tcttatggac cctgttttac aacggctttg ccgccctgtc 2281 ttgggatctg tttgctcagg atacgccggc gcccggttcc gaaggtggcg gcctgcgcaa 2341 cgcgattgtg gggtctttgc tgatcgttgg agccaccgct ctggtctcca ctccggtcgg 2401 catttttgcc gggatctacc tcgcagagtt tggtggtaag tcgcgttttg ccgctgcaac 2461 gcgtttcgta accgatatca tgttgagcgc cccatcgatc gttgtcggtc tttttgttta 2521 tgccctggca gtcgaaaccc tcggcggttt ttccggctgg gccggttccc tggcgttaag 2581 cctgatcgct attccggtag ttgtccgcac gaccgaaaac atgctgctgt tagtgccggg 2641 ctcgctgcgt gaagcggcct ttgcggtcgg cgccccgcac tggcaggtca gcctgaaagt 2701 gaccttacgc gccgtcaaag cgggtgtcct taccggcatt ctgctggcca tcgcccgtgt 2761 gaccggtgaa actgcaccgc tgctgttcac cgcactgaat aatcagtttt atagcactga 2821 catgtcgcag ccgatgggca atctgcctgt tgtgattttc cagtttgcta tgtcaccgta 2881 tgacaactgg gtcaatctgg cgtggggcgg agccttactc attgcgctga tggtgctcgc 2941 gatcaacatt gtcgcacgtg tcctgttccg ccgcaaatcc tcagactaat gatacgagaa 3001 agaggagaaa tcaagtatgt cggcccacca gcaggccgtg gatggcgaag cgattctggc 3061 agttcgtgac ttaagctttt attacggcga ttttcgtggg ttaaataaag tatcacttga 3121 tattgcccag aacaaagtga ccgcgtttat tggtccttcc ggttgtggga agagcaccct 3181 cttgcgtacg ttcaaccgca tgtatgatct gtatccgggg caacgtgcag aaggccaaat 3241 catttttcat gggcgtaatc tgctggaccg taaacaggat gtgaatcttc ttcgtgctcg 3301 tatcggtatg gtattccaga agccaacgcc attcccgatg actatttacg agaacattgc 3361 attcggggtg cgtctgtacg aaccgctggc caaatcgggc atggatgaac gcgtggaatg 3421 ggccctgcgc aaggccgcac tgtgggaaga aacccgtaat aaactgcaac agtcaggact 3481 gagtctgagt ggcggtcagc agcaacgtct gtgtattgct cgcaccatcg ctgtaaaacc 3541 agacgtggtg ttacttgatg aaccgacgag cgctttagat cctctgagta ctgcaaaaat 3601 tgaagaactg attaatgagc tcaagagtga ctacagcatc gcaattgtca cccacaatat 3661 gcagcaagct gcccgcatta gcgactatac cgcgttcatg taccttggag aactgattga 3721 atttggcaat acgcgtgaac tctttgtaaa accgaagaaa cagcaaacgg aagactacat 3781 cacgggtcgc ttcgggtaat gagggccagg catcaaataa aacgaaaggc tcagtcgaaa 3841 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 3901 ggctcacctt cgggtgggcc tttctgcgtt tata //