BBa_K1808000 1 BBa_K1808000 DBR2 gene from Artemisia annua 2015-09-15T11:00:00Z 2015-09-16T08:55:06Z Artemissia annua This is the coding region of the Dbr2 gene from Artemisia annua with added biobrick suffix and prefix in a pSB1C3 plasmid backbone. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The Dbr2 gene encodes the enzyme artemisinic aldehyde delta11(13)-reductase, which catalyses the conversion of artemisinic aldehyde to (11R)-dihydroartemisinic aldehyde with NADPH as the electron donor substrate. The product can be acted upon by the Artemisia annua aldehyde dehydrogenase 1 enzyme (see part BBa_K1808001) to give rise to dihydroartemisinic acid, which can be isolated and converted to the anti-malarial artemisinin. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells. false false _2233_ 26113 26113 9 false This part is BioBrick 3A assembly, E. coli codon and synthesis optimised. false Arnas Petrauskas annotation2460888 1 DBR2 range2460888 1 1 1164 BBa_K1808000_sequence 1 atgtctgaaaaaccaacattatttagcgcgtataaaatggggaaatttaatctgtcgcaccgcgtggtgctggcgccaatgacccgttgccgcgccattaacgccattccgaatgaagccctggtggaatattatcgccaacgcagtaccgcaggtggctttcttatcactgaaggaaccatgatctctccctcttctgcgggctttccacatgtaccgggcatttttactaaagagcaagtcgaaggttggaagaaagtcgtggatgcggcccacaaagagggagctgtgatcttctgtcaactctggcatgtcggtcgtgcctctcatcaagtttatcagccgggcggggctgcacctatcagtagcacctcaaaaccaattagtaaaaaatgggaaattctgttacctgatgcaacatatggcacgtacccggaaccgcgtcctctggctgccaacgaaattttagaggttgtcgaagattatcgcgtggcggccattaacgcgatcgaagcgggctttgacggcatcgaaatccatggagcgcatggctatctgcttgatcaattcatgaaagatggtatcaatgatcgtactgatgaatacggaggctctctggaaaaccgctgtaaattcatccttcaagtcgtacaggctgtcagcgcagcaatcggaacggatcgtgtcggtatccgtatcagcccggcaattgaccataccgacgccatggacagcgatccgcgctccttaggtctggctgttattgagcgcctgaataaacttcagttcaaactgggttcccgcttagcgtatttacatgttacgcaaccacgttatactgccgatggccacggccagacagaagccggggcaaacggctctgaagaggaagtagcgcaactgatgaagacgtggcgcggtgcgtacgtgggcaccttcatctgttgtggcggttatactcgcgagttaggactccaagcggtggctcaaggggacgctgatctggtcgccttcggacgttactttgtaagtaaccctgacttggtactgcgcttaaagcttaatgcgccgctgaaccgctacgatcgtgcaaccttttacactcacgatccggtggtcggctacacggattatccttccctggacaaaggtagtcttttataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z