BBa_K1808003 1 BBa_K1808003 DBR2 gene from Artemisia annua, 6 His tagged 2015-09-15T11:00:00Z 2015-09-16T10:34:22Z Artemisia annua This is the coding region of the Dbr2 gene from Artemisia annua with added biobrick suffix, prefix and a 6 His tag in a pSB1C3 plasmid backbone. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The Dbr2 gene encodes the enzyme artemisinic aldehyde delta11(13)-reductase, which catalyses the conversion of artemisinic aldehyde to (11R)-dihydroartemisinic aldehyde with NADPH as the electron donor substrate. The product can be acted upon by the Artemisia annua aldehyde dehydrogenase 1 enzyme (see part BBa_K1808001) to give rise to dihydroartemisinic acid, which can be isolated and converted to the anti-malarial artemisinin. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification. false false _2233_ 26113 26113 9 false This part is BioBrick 3A assembly, E. coli codon and synthesis optimised. This part is 6 His tagged to allow protein purification. false Arnas Petrauskas annotation2461187 1 6 His range2461187 1 1161 1179 annotation2461186 1 DBR2 6His tagged range2461186 1 1 1182 BBa_K1808003_sequence 1 atgtctgaaaaaccaacattatttagcgcgtataaaatggggaaatttaatctgtcgcaccgcgtggtgctggcgccaatgacccgttgccgcgccattaacgccattccgaatgaagccctggtggaatattatcgccaacgcagtaccgcaggtggctttcttatcactgaaggaaccatgatctctccctcttctgcgggctttccacatgtaccgggcatttttactaaagagcaagtcgaaggttggaagaaagtcgtggatgcggcccacaaagagggagctgtgatcttctgtcaactctggcatgtcggtcgtgcctctcatcaagtttatcagccgggcggggctgcacctatcagtagcacctcaaaaccaattagtaaaaaatgggaaattctgttacctgatgcaacatatggcacgtacccggaaccgcgtcctctggctgccaacgaaattttagaggttgtcgaagattatcgcgtggcggccattaacgcgatcgaagcgggctttgacggcatcgaaatccatggagcgcatggctatctgcttgatcaattcatgaaagatggtatcaatgatcgtactgatgaatacggaggctctctggaaaaccgctgtaaattcatccttcaagtcgtacaggctgtcagcgcagcaatcggaacggatcgtgtcggtatccgtatcagcccggcaattgaccataccgacgccatggacagcgatccgcgctccttaggtctggctgttattgagcgcctgaataaacttcagttcaaactgggttcccgcttagcgtatttacatgttacgcaaccacgttatactgccgatggccacggccagacagaagccggggcaaacggctctgaagaggaagtagcgcaactgatgaagacgtggcgcggtgcgtacgtgggcaccttcatctgttgtggcggttatactcgcgagttaggactccaagcggtggctcaaggggacgctgatctggtcgccttcggacgttactttgtaagtaaccctgacttggtactgcgcttaaagcttaatgcgccgctgaaccgctacgatcgtgcaaccttttacactcacgatccggtggtcggctacacggattatccttccctggacaaaggtagtcttttacatcaccatcaccatcactaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z