BBa_K1808005 1 BBa_K1808005 ADH1 HISTAG from artemisia annua 6 His tagged 2015-09-15T11:00:00Z 2015-09-16T10:45:43Z Artemisia annua This is the coding region of the ADH1 gene from Artemisia annua with added biobrick suffix and prefix in a pSB1C3 plasmid backbone with a 6 His tag. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The Dbr2 gene encodes the enzyme alcohol dehydrogenase 1, which catalyses the conversion of artemisinic alcohol to artemisinic aldehyde with NAD+ as the electron donor substrate. The product can be acted upon by the Artemisia annua DBR2 enzyme (see part BBa_K1808000) to give rise to dihydroartemisinic acid, which can be isolated and converted to the anti-malarial artemisinin. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification. false false _2233_ 26124 26124 9 false This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification. false Remsha Afzal annotation2461165 1 ADH1 HIS range2461165 1 1 1155 annotation2461221 1 6 HIS range2461221 1 1135 1152 BBa_K1808005_sequence 1 atggcgcagaaagcacctggggtgatcacatgtaaagcggcagttgtttgggaaagtagtggcccggtcgtcctggaggagattcgcgttgatccgccgaaagcgagtgaagtgcgtattaaaatgctgtgtgcttccctgtgccacaccgatgtgctttgtaccaaaggctttccgatcccgttgtttccccggattccaggccatgagggagtcggtgtgattgagagcattggcaaggatgcgaaagggttgaaaccaggtgacattgtgatgccgctctatctgggcgagtgtggccaatgtctcaactgcaaaacgggcaagactaacctgtgtcacgtctatccgccctcctttagcggcctcatgaatgatggaacctctcgtatgtcaattgcgcgcactggtgaatcgatttatcattttgcctcgtgcagcacgtggaccgaatacgccgtggcggattgcaattatgttctgaagatcaacccgaagatttcgtatccacacgccagcttcctgagctgcggcttcacgacgggatttggtgccacgtggcgggaaactcaggtttcgaaaggttctagtgtggccgtcttcggtatcggcacagttggtcttggcgtgattaaaggtgcgcagttacaaggagcctctaaaattattggagtcgacgtgaatcagtacaaagccgccaaagggaaagtatttggcatgacggattttattaacccgaaggaccatccggataaatcagtctctgaattggtcaaagaactgacacatggtctgggtgtggaccattgttttgaatgtacaggtgtaccgtctttgcttaatgaagccttggaagcgagcaaaatcggtattggaaccgtggtgccgatcggcgccggcggcgaagctagtgttgccattaactcattgatcctcttcagtggacgcacgctgaaatttacagccttcggcggggtacgtacgcagagcgacctgccagtcattatcgataagtgtcttaacaaagaaatccagctggacgaattgctcacgcatgagatccaccttgataacattcaggaggcattcgagatccttaagaaacctgactgcgtgaaaatcctgatcaagttccatcaccatcaccatcactaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z