BBa_K1808007 1 BBa_K1808007 CYP71AV1 from Artemisia annua 2015-09-15T11:00:00Z 2015-09-16T11:26:10Z Artemisia annua This is the coding region of the CYP71AV1 gene from Artemisia annua with added biobrick suffix, prefix and a 6 His tag in a pSB1C3 plasmid backbone. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The CYP71AV1 gene encodes the enzyme Amorphadiene oxidase, which catalyses the conversion of amorpha-4,11-diene to artemisinic alcohol, subsequent conversion of the alcohol to artemisinic aldehyde and the subsequent and final conversion of the artemisinic aldehyde to artemisinic acid with NADPH as the substrate. Artemisinic acid and dihydroartemisinic acid can be extracted from E. coli cells through lysis and chemically converted to the anti-malarial artemisinin. The Amorphadiene oxidase enzyme is a memberofthe cytochrome p450 family of hemoproteins and is the rate limiting enzyme in the synthesis pathway for artemisinic acid developed by Amyris. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification. false false _2233_ 26113 26113 9 false This part is BioBrick 3A assembly, E. coli codon and synthesis optimised. This part is 6 His tagged to allow protein purification. false Arnas Petrauskas annotation2461371 1 6 His range2461371 1 1485 1503 annotation2461370 1 CYP71AV1 6 His tagged range2461370 1 1 1506 BBa_K1808007_sequence 1 atgaaaagcattttgaaagctatggccctttcactgacgacctcgattgcacttgcgacgattctgctttttgtatataaattcgcaacccgctcgaaaagtactaaaaaatcgttgccagaaccttggcgtcttccgatcattggtcacatgcatcatctgatcggcacgaccccacatcgcggcgtgcgcgatctggctcgcaaatacggctcgttaatgcatctgcaattaggtgaggtgccgaccattgtggtaagcagtcccaaatgggccaaagagatccttacgacgtatgacatcacctttgcgaatcgccccgaaaccttaactggcgaaattgtgttgtatcacaatacggatgtggtcctggcgccgtatggggaatattggcgccagctgcgtaaaatctgcacccttgagctgttatccgttaagaaggtgaagagtttccaatcattgcgtgaagaagagtgttggaacttggttcaagagattaaagcatcaggatcgggccggccggtcaacctgtccgagaacgttttcaaactgatcgccacaattctctctcgcgccgcgtttgggaaaggtatcaaggatcaaaaagaactgaccgagattgtcaaagaaattctccgccagactggcggtttcgatgtggccgatatttttccgtctaaaaaatttctgcaccatttatccggcaagcgcgctcgtctcacctcactgcgcaaaaaaattgataacctgatcgacaatttggtggcagaacatacagtaaatacatcgagtaagaccaatgagacgctgttggatgttttgctgcgtctcaaagattccgccgagtttccactgacgtccgataacattaaagcaattattctggacatgtttggggcaggcaccgacaccagcagctcaacgatcgaatgggccatctctgagttgattaaatgtcccaaagctatggaaaaagtgcaggccgaactgcgtaaggcattgaacgggaaagaaaaaattcatgaagaagatattcaagagcttagttacctgaatatggtgattaaagaaacactgcggctccacccgccactcccgctggtcctgccgcgggaatgtcgccaacctgtgaatcttgcaggttacaacattcccaataagactaaattgatcgtcaatgtgtttgcgatcaatcgcgacccggaatattggaaagatgccgaagccttcatcccggagcgtttcgaaaatagtagcgctaccgtaatgggcgcggagtacgaatacttaccgtttggtgcaggacgtcgtatgtgcccaggcgcggctctcgggctggctaatgtgcaactgcctttggcgaacattttataccattttaactggaaactgccgaatggggtgagttatgatcaaatcgacatgaccgaaagcagtggggcgaccatgcagcgtaagacggaacttctgctggttccgtctttccatcaccatcaccatcactaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z