BBa_K1808009 1 BBa_K1808009 CYTOCHROME P450 REDUCTASE HIS TAGGED 2015-09-15T11:00:00Z 2015-09-16T11:42:57Z Artemisia annua This is the coding region of the CPR gene from Artemisia annua with added biobrick suffix and prefix in a pSB1C3 plasmid backbone as well as a 6 his tagged component. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The CPR gene encodes the enzyme Cytochrome P450 reductase, which catalyses the conversion of NADPH back to NAD+ by transference of electron between Cytochrome P450. The product is used in the production of dihydroartemisinic acid, which can be isolated and converted to the anti-malarial artemisinin. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification. false false _2233_ 26124 26124 9 false This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification. false Remsha Afzal annotation2461425 1 CPR range2461425 1 1 2133 annotation2461426 1 6 HIS TAG range2461426 1 2113 2130 BBa_K1808009_sequence 1 atgcagtccacgaccagcgttaaactcagccctttcgatctgatgactgcactcttaaatggcaaggttagcttcgacacttcgaacacctctgacaccaatattccgctggcagtgtttatggaaaaccgtgaactgttgatgatcttgactacaagtgtggcagtgctgatcggctgcgtggtggtcctggtgtggcgtcgctctagctctgcggccaaaaaagcggccgaaagtccggttatcgtcgtcccgaaaaaagtcactgaagacgaagtagacgatgggcgcaagaaggtcacggtgttctttggcacacaaactggtaccgcagaaggctttgcgaaggcgttagtagaagaagcaaaagcgcggtacgaaaaggcggtctttaaagttattgatctggatgattacgccgcggaagacgatgaatatgaggaaaaattaaaaaaggaaagtctggcgttcttctttctcgcaacatatggcgacggcgagccaaccgacaatgcggcgcgcttctacaaatggtttacagaaggtgaagagaaaggagaatggctggataagcttcagtatgcagtgtttgggctggggaaccgccaatatgaacactttaataaaattgcaaaagtagtggatgagaaactggtagagcagggcgcgaaacgtttagttccagtcggaatgggcgatgatgatcaatgcattgaggacgatttcacggcctggaaagaactggtgtggcccgaactggatcagttgctccgtgatgaagatgataccagcgtggcgacgccgtatacggccgcagttggtgaatatcgtgtagtttttcacgataaaccggagacttacgatcaggaccaactcacgaacggccatgctgtacatgatgcgcagcatccttgccgctctaatgtggccgtcaaaaaagaactgcacagcccactgtccgaccggtcatgcacccacttagagtttgacatctctaacaccggcctcagctatgaaactggcgatcacgttggtgtgtatgttgaaaacctgtctgaggtcgttgatgaagcggaaaaattaattggcctcccgcctcatacctacttctctgttcataccgataatgaagatggcacgcccctggggggcgcgtcactgccaccgcctttcccgccgtgcacacttcggaaagcgctggcttcgtatgctgatgtcttatctagcccgaaaaaatccgctctgctggccttagccgctcatgcaaccgactctacggaagcggatcgtctgaaattctttgcgagtccggccggaaaagatgaatatgctcaatggattgtggcgagtcatcggtctttattagaagtcatggaggcctttccaagcgcgaaaccgccactgggggtttttttcgcttcagtcgcccctcggttacaaccgcgttactactccatttcatccagtccaaagtttgcaccgaaccgcatccatgtcacctgcgcgctcgtttatgaacaaacccccagcggtcgggttcacaaaggcgtctgctcgacgtggatgaaaaacgcggtaccgatgaccgaatcgcaggactgttcatgggccccaatttacgttcgtacctcaaacttccgcctgcctagcgatccgaaagtgcccgttattatgatcggtccgggaaccggtctggctcccttccggggatttctccaggaacgtctggcccagaaagaagctggcacagagctcggtactgccatcctgtttttcggctgtcgtaaccgcaaagttgacttcatctacgaagatgaactgaacaattttgttgagaccggcgcactgagcgaattggtcaccgcgtttagccgcgaaggcgcgacgaaagaatacgtgcagcacaagatgactcagaaagcttctgacatctggaatcttctctccgagggcgcctatctgtatgtttgtggagatgctaagggtatggccaaagatgtgcaccgtacattgcataccatcgtgcaggaacaggggtcgttagacagttcaaaagctgagctgtatgtcaaaaatctgcaaatggcggggcgctacctgcgcgatgtttggcatcaccatcaccatcactaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z