LOCUS       BBa_K1808010            1717 bp    DNA     linear       23-May-2024
DEFINITION  ALDH1 generator
ACCESSION   BBa_K1808010
VERSION     BBa_K1808010.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=J23101
     misc_feature    1..6
                     /label=-35 box
     misc_feature    24..29
                     /label=-10 box
     promoter        1..35
                     /label=BBa_K823005
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             63..1580
                     /label=ALDH1 6 His tagged
     misc_feature    1559..1577
                     /label=6 His
     CDS             63..1580
                     /label=BBa_K1808004
     misc_feature    44..1580
                     /label=BBa_S05303
     stem_loop       1600..1643
                     /label=stem_loop
     misc_feature    1589..1668
                     /label=BBa_B0010
     terminator      1589..1668
                     /label=BBa_B0010
     stem_loop       1684..1703
                     /label=T7 TE
     misc_feature    1710..1710
                     /label=stop
     polyA_site      1704..1717
                     /label=polya
     misc_feature    1677..1717
                     /label=BBa_B0012
     terminator      1677..1717
                     /label=BBa_B0012
     terminator      1589..1717
                     /label=BBa_B0015
     misc_feature    44..1717
                     /label=BBa_S05306
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatgtcctc aggggcgaat ggctcgagta agtctgcatc acataaaatc aaattcacta
      121 aactgtttat taacggtgaa tttgtagatt cgatcagtgg taacaccttc gataccatca
      181 accctgcgac tgaagaagtg ctggcaacag ttgcggaggg acgcaaagaa gatattgacc
      241 tggctgtgaa agctgcgcgc gaggcctttg ataacggtcc ctggccgcgg atgtctgggg
      301 aggcgcggcg caagatcatg ctgaaatttg ccgatttaat tgacgaaaac gccgacgaac
      361 tgacgacact tgaagtaatt gatggcggga agttgtttgg tcctgtgcgt cactttgagg
      421 ttccggtgtc tagcgatact tttcgttact tcgccggtgc agcagataaa attcgtgggg
      481 cgacgctgaa aatgtcttcg aatatccagg cgtacacttt acgcgagccg attggcgtag
      541 taggtcacat catcccgtgg aacggtccag ccttcatgtt tgcaaccaag gtagctccgg
      601 cactcgcggc gggatgcacc atggtgatta agccagcgga acacacacca ctgacggtgc
      661 tgtttctggc gcacctgagc aaactcgcgg gagttccgga cggtgttatc aacgttgtca
      721 acgggtttgg caaaacggct ggcgcggcgg ttagctcaca tatggacatc gacatggtga
      781 ccttcaccgg atcaaccgag gtgggtcgta cggtgatgca ggctgccgct ctgagcaacc
      841 tgaagccggt atcgctggag ctggggggca aaagcccgct gatcgttttt gacgatgccg
      901 atgtggacaa agctgccgaa tttgccatct tgggtaattt taccaacaaa ggagaaatgt
      961 gtgttgctgg aagccgtgtt tttgtgcagg agggcatcca tgatgtcttc gtgaaaaagc
     1021 tggaaggggc tgttaaagcc tgggcgacac gtgacccatt cgacctggcc acccgtcatg
     1081 gcccgcaaaa caataaacag caatatgaca aagtactcag ttgtatcaat catggaaaaa
     1141 aagaaggggc gaccctggtt acgggcggca agccctttgg gaaaaaaggc tattatatcg
     1201 aacctaccct gttcacgaat gtcaccgatg acatgaccat cgcgaaagaa gaaatctttg
     1261 ggcctgtcat tagcgtgtta aaattcaaaa cagtggagga ggttattaaa cgtgccaacg
     1321 cgacgaagta tggtctcgcg tcaggcgtgt tcactaagaa tattgatgta gtgaatacgg
     1381 tgagccgttc aatccgtgcg ggtgcggttt gggtcaattg ttatttagcc ttggaccggg
     1441 acgcacctca tggtgggtat aaaatgtcgg ggttcggccg cgaacagggg ttagaagcgc
     1501 tggaacatta tttacagatt aaaaccgtgg caacgccaat ctacgatagc ccatggctcc
     1561 atcaccatca ccatcactaa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1621 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1681 actggctcac cttcgggtgg gcctttctgc gtttata
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