LOCUS       BBa_K1808012            2907 bp    DNA     linear       22-Nov-2024
DEFINITION  DBR2 and ALDH1 generator "Alpha"
ACCESSION   BBa_K1808012
VERSION     BBa_K1808012.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=J23101
     misc_feature    1..6
                     /label=-35 box
     misc_feature    24..29
                     /label=-10 box
     promoter        1..35
                     /label=BBa_K823005
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1225
                     /label=DBR2
     CDS             62..1225
                     /label=BBa_K1808000
     misc_feature    44..1225
                     /label=BBa_S05304
     misc_feature    1..1225
                     /label=BBa_S05305
     RBS             1234..1246
                     /label=RBS-3\Weak
     RBS             1240..1243
                     /label=RBS
     misc_feature    1234..1246
                     /label=BBa_B0032
     RBS             1234..1246
                     /label=BBa_B0032
     CDS             1253..2770
                     /label=ALDH1 6 His tagged
     misc_feature    2749..2767
                     /label=6 His
     CDS             1253..2770
                     /label=BBa_K1808004
     misc_feature    1234..2770
                     /label=BBa_S05303
     stem_loop       2790..2833
                     /label=stem_loop
     misc_feature    2779..2858
                     /label=BBa_B0010
     terminator      2779..2858
                     /label=BBa_B0010
     stem_loop       2874..2893
                     /label=T7 TE
     misc_feature    2900..2900
                     /label=stop
     polyA_site      2894..2907
                     /label=polya
     misc_feature    2867..2907
                     /label=BBa_B0012
     terminator      2867..2907
                     /label=BBa_B0012
     terminator      2779..2907
                     /label=BBa_B0015
     misc_feature    1234..2907
                     /label=BBa_S05306
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgtctgaa aaaccaacat tatttagcgc gtataaaatg gggaaattta atctgtcgca
      121 ccgcgtggtg ctggcgccaa tgacccgttg ccgcgccatt aacgccattc cgaatgaagc
      181 cctggtggaa tattatcgcc aacgcagtac cgcaggtggc tttcttatca ctgaaggaac
      241 catgatctct ccctcttctg cgggctttcc acatgtaccg ggcattttta ctaaagagca
      301 agtcgaaggt tggaagaaag tcgtggatgc ggcccacaaa gagggagctg tgatcttctg
      361 tcaactctgg catgtcggtc gtgcctctca tcaagtttat cagccgggcg gggctgcacc
      421 tatcagtagc acctcaaaac caattagtaa aaaatgggaa attctgttac ctgatgcaac
      481 atatggcacg tacccggaac cgcgtcctct ggctgccaac gaaattttag aggttgtcga
      541 agattatcgc gtggcggcca ttaacgcgat cgaagcgggc tttgacggca tcgaaatcca
      601 tggagcgcat ggctatctgc ttgatcaatt catgaaagat ggtatcaatg atcgtactga
      661 tgaatacgga ggctctctgg aaaaccgctg taaattcatc cttcaagtcg tacaggctgt
      721 cagcgcagca atcggaacgg atcgtgtcgg tatccgtatc agcccggcaa ttgaccatac
      781 cgacgccatg gacagcgatc cgcgctcctt aggtctggct gttattgagc gcctgaataa
      841 acttcagttc aaactgggtt cccgcttagc gtatttacat gttacgcaac cacgttatac
      901 tgccgatggc cacggccaga cagaagccgg ggcaaacggc tctgaagagg aagtagcgca
      961 actgatgaag acgtggcgcg gtgcgtacgt gggcaccttc atctgttgtg gcggttatac
     1021 tcgcgagtta ggactccaag cggtggctca aggggacgct gatctggtcg ccttcggacg
     1081 ttactttgta agtaaccctg acttggtact gcgcttaaag cttaatgcgc cgctgaaccg
     1141 ctacgatcgt gcaacctttt acactcacga tccggtggtc ggctacacgg attatccttc
     1201 cctggacaaa ggtagtcttt tataatacta gagtcacaca ggaaagtact agatgtcctc
     1261 aggggcgaat ggctcgagta agtctgcatc acataaaatc aaattcacta aactgtttat
     1321 taacggtgaa tttgtagatt cgatcagtgg taacaccttc gataccatca accctgcgac
     1381 tgaagaagtg ctggcaacag ttgcggaggg acgcaaagaa gatattgacc tggctgtgaa
     1441 agctgcgcgc gaggcctttg ataacggtcc ctggccgcgg atgtctgggg aggcgcggcg
     1501 caagatcatg ctgaaatttg ccgatttaat tgacgaaaac gccgacgaac tgacgacact
     1561 tgaagtaatt gatggcggga agttgtttgg tcctgtgcgt cactttgagg ttccggtgtc
     1621 tagcgatact tttcgttact tcgccggtgc agcagataaa attcgtgggg cgacgctgaa
     1681 aatgtcttcg aatatccagg cgtacacttt acgcgagccg attggcgtag taggtcacat
     1741 catcccgtgg aacggtccag ccttcatgtt tgcaaccaag gtagctccgg cactcgcggc
     1801 gggatgcacc atggtgatta agccagcgga acacacacca ctgacggtgc tgtttctggc
     1861 gcacctgagc aaactcgcgg gagttccgga cggtgttatc aacgttgtca acgggtttgg
     1921 caaaacggct ggcgcggcgg ttagctcaca tatggacatc gacatggtga ccttcaccgg
     1981 atcaaccgag gtgggtcgta cggtgatgca ggctgccgct ctgagcaacc tgaagccggt
     2041 atcgctggag ctggggggca aaagcccgct gatcgttttt gacgatgccg atgtggacaa
     2101 agctgccgaa tttgccatct tgggtaattt taccaacaaa ggagaaatgt gtgttgctgg
     2161 aagccgtgtt tttgtgcagg agggcatcca tgatgtcttc gtgaaaaagc tggaaggggc
     2221 tgttaaagcc tgggcgacac gtgacccatt cgacctggcc acccgtcatg gcccgcaaaa
     2281 caataaacag caatatgaca aagtactcag ttgtatcaat catggaaaaa aagaaggggc
     2341 gaccctggtt acgggcggca agccctttgg gaaaaaaggc tattatatcg aacctaccct
     2401 gttcacgaat gtcaccgatg acatgaccat cgcgaaagaa gaaatctttg ggcctgtcat
     2461 tagcgtgtta aaattcaaaa cagtggagga ggttattaaa cgtgccaacg cgacgaagta
     2521 tggtctcgcg tcaggcgtgt tcactaagaa tattgatgta gtgaatacgg tgagccgttc
     2581 aatccgtgcg ggtgcggttt gggtcaattg ttatttagcc ttggaccggg acgcacctca
     2641 tggtgggtat aaaatgtcgg ggttcggccg cgaacagggg ttagaagcgc tggaacatta
     2701 tttacagatt aaaaccgtgg caacgccaat ctacgatagc ccatggctcc atcaccatca
     2761 ccatcactaa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     2821 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     2881 cttcgggtgg gcctttctgc gtttata
//