##gff-version 3
##sequence-region BBa_K1808012 1 2907
BBa_K1808012	.	engineered_region	1	1225	.	+	0	ID=BBa_S05305;Name=BBa_S05305
BBa_K1808012	.	promoter	1	35	.	+	0	ID=BBa_K823005;Name=BBa_K823005;Parent=BBa_S05305
BBa_K1808012	.	promoter	1	35	.	+	0	ID=annotation2182509;Name=J23101;Parent=BBa_K823005
BBa_K1808012	.	non_covalent_binding_site	1	6	.	+	0	ID=annotation2182510;Name=-35 box;Parent=BBa_K823005
BBa_K1808012	.	non_covalent_binding_site	24	29	.	+	0	ID=annotation2182511;Name=-10 box;Parent=BBa_K823005
BBa_K1808012	.	engineered_region	44	1225	.	+	0	ID=BBa_S05304;Name=BBa_S05304;Parent=BBa_S05305
BBa_K1808012	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S05304
BBa_K1808012	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1808012	.	CDS	62	1225	.	+	0	ID=BBa_K1808000;Name=BBa_K1808000;Parent=BBa_S05304
BBa_K1808012	.	CDS	62	1225	.	+	0	ID=annotation2460888;Name=DBR2;Parent=BBa_K1808000
BBa_K1808012	.	engineered_region	1234	2907	.	+	0	ID=BBa_S05306;Name=BBa_S05306
BBa_K1808012	.	terminator	2779	2907	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S05306
BBa_K1808012	.	terminator	2867	2907	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1808012	.	polyA_site	2894	2907	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1808012	.	stop_codon	2900	2900	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1808012	.	stem_loop	2874	2893	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1808012	.	engineered_region	2867	2907	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1808012	.	terminator	2779	2858	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1808012	.	stem_loop	2790	2833	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1808012	.	engineered_region	2779	2858	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1808012	.	engineered_region	1234	2770	.	+	0	ID=BBa_S05303;Name=BBa_S05303;Parent=BBa_S05306
BBa_K1808012	.	CDS	1253	2770	.	+	0	ID=BBa_K1808004;Name=BBa_K1808004;Parent=BBa_S05303
BBa_K1808012	.	tag	2749	2767	.	+	0	ID=annotation2461246;Name=6 His;Parent=BBa_K1808004
BBa_K1808012	.	CDS	1253	2770	.	+	0	ID=annotation2461243;Name=ALDH1 6 His tagged;Parent=BBa_K1808004
BBa_K1808012	.	ribosome_entry_site	1234	1246	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05303
BBa_K1808012	.	engineered_region	1234	1246	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1808012	.	ribosome_entry_site	1234	1246	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1808012	.	ribosome_entry_site	1240	1243	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
>BBa_K1808012
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgtctgaaaaaccaaca
ttatttagcgcgtataaaatggggaaatttaatctgtcgcaccgcgtggtgctggcgccaatgacccgttgccgcgcca
ttaacgccattccgaatgaagccctggtggaatattatcgccaacgcagtaccgcaggtggctttcttatcactgaagg
aaccatgatctctccctcttctgcgggctttccacatgtaccgggcatttttactaaagagcaagtcgaaggttggaag
aaagtcgtggatgcggcccacaaagagggagctgtgatcttctgtcaactctggcatgtcggtcgtgcctctcatcaag
tttatcagccgggcggggctgcacctatcagtagcacctcaaaaccaattagtaaaaaatgggaaattctgttacctga
tgcaacatatggcacgtacccggaaccgcgtcctctggctgccaacgaaattttagaggttgtcgaagattatcgcgtg
gcggccattaacgcgatcgaagcgggctttgacggcatcgaaatccatggagcgcatggctatctgcttgatcaattca
tgaaagatggtatcaatgatcgtactgatgaatacggaggctctctggaaaaccgctgtaaattcatccttcaagtcgt
acaggctgtcagcgcagcaatcggaacggatcgtgtcggtatccgtatcagcccggcaattgaccataccgacgccatg
gacagcgatccgcgctccttaggtctggctgttattgagcgcctgaataaacttcagttcaaactgggttcccgcttag
cgtatttacatgttacgcaaccacgttatactgccgatggccacggccagacagaagccggggcaaacggctctgaaga
ggaagtagcgcaactgatgaagacgtggcgcggtgcgtacgtgggcaccttcatctgttgtggcggttatactcgcgag
ttaggactccaagcggtggctcaaggggacgctgatctggtcgccttcggacgttactttgtaagtaaccctgacttgg
tactgcgcttaaagcttaatgcgccgctgaaccgctacgatcgtgcaaccttttacactcacgatccggtggtcggcta
cacggattatccttccctggacaaaggtagtcttttataatactagagtcacacaggaaagtactagatgtcctcaggg
gcgaatggctcgagtaagtctgcatcacataaaatcaaattcactaaactgtttattaacggtgaatttgtagattcga
tcagtggtaacaccttcgataccatcaaccctgcgactgaagaagtgctggcaacagttgcggagggacgcaaagaaga
tattgacctggctgtgaaagctgcgcgcgaggcctttgataacggtccctggccgcggatgtctggggaggcgcggcgc
aagatcatgctgaaatttgccgatttaattgacgaaaacgccgacgaactgacgacacttgaagtaattgatggcggga
agttgtttggtcctgtgcgtcactttgaggttccggtgtctagcgatacttttcgttacttcgccggtgcagcagataa
aattcgtggggcgacgctgaaaatgtcttcgaatatccaggcgtacactttacgcgagccgattggcgtagtaggtcac
atcatcccgtggaacggtccagccttcatgtttgcaaccaaggtagctccggcactcgcggcgggatgcaccatggtga
ttaagccagcggaacacacaccactgacggtgctgtttctggcgcacctgagcaaactcgcgggagttccggacggtgt
tatcaacgttgtcaacgggtttggcaaaacggctggcgcggcggttagctcacatatggacatcgacatggtgaccttc
accggatcaaccgaggtgggtcgtacggtgatgcaggctgccgctctgagcaacctgaagccggtatcgctggagctgg
ggggcaaaagcccgctgatcgtttttgacgatgccgatgtggacaaagctgccgaatttgccatcttgggtaattttac
caacaaaggagaaatgtgtgttgctggaagccgtgtttttgtgcaggagggcatccatgatgtcttcgtgaaaaagctg
gaaggggctgttaaagcctgggcgacacgtgacccattcgacctggccacccgtcatggcccgcaaaacaataaacagc
aatatgacaaagtactcagttgtatcaatcatggaaaaaaagaaggggcgaccctggttacgggcggcaagccctttgg
gaaaaaaggctattatatcgaacctaccctgttcacgaatgtcaccgatgacatgaccatcgcgaaagaagaaatcttt
gggcctgtcattagcgtgttaaaattcaaaacagtggaggaggttattaaacgtgccaacgcgacgaagtatggtctcg
cgtcaggcgtgttcactaagaatattgatgtagtgaatacggtgagccgttcaatccgtgcgggtgcggtttgggtcaa
ttgttatttagccttggaccgggacgcacctcatggtgggtataaaatgtcggggttcggccgcgaacaggggttagaa
gcgctggaacattatttacagattaaaaccgtggcaacgccaatctacgatagcccatggctccatcaccatcaccatc
actaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata