BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 annotation7027 1 BBa_B0032 range7027 1 1 13 BBa_K823005 1 BBa_K823005 Anderson promoter J23101 2012-09-07T11:00:00Z 2015-07-07T11:44:25Z Partsregistry Released HQ 2013 Anderson promoter J23101 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon. false false _1081_ 4206 12081 9 In stock false no considerations false Korinna Kraft annotation2182510 1 -35 box range2182510 1 1 6 annotation2182509 1 J23101 range2182509 1 1 35 annotation2182511 1 -10 box range2182511 1 24 29 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_S05305 1 BBa_S05305 K823005:S05304 2015-09-15T11:00:00Z 2015-09-16T11:46:21Z false false _9_ 26124 26124 9 false false Remsha Afzal component2461446 1 BBa_K823005 component2461451 1 BBa_S05304 annotation2461446 1 BBa_K823005 range2461446 1 1 35 annotation2461451 1 BBa_S05304 range2461451 1 44 1225 BBa_K1808012 1 BBa_K1808012 DBR2 and ALDH1 generator "Alpha" 2015-09-15T11:00:00Z 2015-09-16T12:20:28Z Artemisia annua The Alpha construct false false _2233_ 26113 26113 9 false This part is BioBrick 3A assembly, E. coli codon and synthesis optimised. This part is partially 6 His tagged to allow purification of ALDH1 protein. false Arnas Petrauskas component2461599 1 BBa_S05305 component2461614 1 BBa_S05306 annotation2461599 1 BBa_S05305 range2461599 1 1 1225 annotation2461614 1 BBa_S05306 range2461614 1 1234 2907 BBa_S05304 1 BBa_S05304 B0034:K1808000 2015-09-15T11:00:00Z 2015-09-16T11:43:55Z false false _9_ 26125 26125 9 false false D??ire Gannon component2461430 1 BBa_K1808000 component2461428 1 BBa_B0034 annotation2461430 1 BBa_K1808000 range2461430 1 19 1182 annotation2461428 1 BBa_B0034 range2461428 1 1 12 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1808000 1 BBa_K1808000 DBR2 gene from Artemisia annua 2015-09-15T11:00:00Z 2015-09-16T08:55:06Z Artemissia annua This is the coding region of the Dbr2 gene from Artemisia annua with added biobrick suffix and prefix in a pSB1C3 plasmid backbone. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The Dbr2 gene encodes the enzyme artemisinic aldehyde delta11(13)-reductase, which catalyses the conversion of artemisinic aldehyde to (11R)-dihydroartemisinic aldehyde with NADPH as the electron donor substrate. The product can be acted upon by the Artemisia annua aldehyde dehydrogenase 1 enzyme (see part BBa_K1808001) to give rise to dihydroartemisinic acid, which can be isolated and converted to the anti-malarial artemisinin. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells. false false _2233_ 26113 26113 9 false This part is BioBrick 3A assembly, E. coli codon and synthesis optimised. false Arnas Petrauskas annotation2460888 1 DBR2 range2460888 1 1 1164 BBa_S05303 1 BBa_S05303 B0032:K1808004 2015-09-15T11:00:00Z 2015-09-16T11:34:09Z false false _9_ 26125 26125 9 false false D??ire Gannon component2461382 1 BBa_B0032 component2461386 1 BBa_K1808004 annotation2461382 1 BBa_B0032 range2461382 1 1 13 annotation2461386 1 BBa_K1808004 range2461386 1 20 1537 BBa_S05306 1 BBa_S05306 S05303:B0015 2015-09-15T11:00:00Z 2015-09-16T11:47:49Z false false _9_ 26113 26113 9 false false Arnas Petrauskas component2461465 1 BBa_B0015 component2461458 1 BBa_S05303 annotation2461465 1 BBa_B0015 range2461465 1 1546 1674 annotation2461458 1 BBa_S05303 range2461458 1 1 1537 BBa_K1808004 1 BBa_K1808004 ALDH1 from Artemisia annua, 6 His tagged 2015-09-15T11:00:00Z 2015-09-18T06:12:06Z Artemisia annua This is the coding region of the ALDH1 gene from Artemisia annua with added biobrick suffix, prefix and a 6 His tag in a pSB1C3 plasmid backbone. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The ALDH1 gene encodes the enzyme aldehyde dehydrogenase 1, which catalyses the conversion of (11R)-dihydroartemisinic aldehyde to dihydroartemisinic acid as well as the conversion of artemisinic aldehyde to artemisinic acid with NADP+ as the substrate. Artemisinic acid and dihydroartemisinic acid can be extracted from E. coli cells through lysis and chemically converted to the anti-malarial artemisinin. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification. false false _2233_ 26113 26113 9 true This part is BioBrick 3A assembly, E. coli codon and synthesis optimised. This part is 6 His tagged to allow protein purification. false Arnas Petrauskas annotation2461243 1 ALDH1 6 His tagged range2461243 1 1 1518 annotation2461246 1 6 His range2461246 1 1497 1515 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_S05304_sequence 1 aaagaggagaaatactagatgtctgaaaaaccaacattatttagcgcgtataaaatggggaaatttaatctgtcgcaccgcgtggtgctggcgccaatgacccgttgccgcgccattaacgccattccgaatgaagccctggtggaatattatcgccaacgcagtaccgcaggtggctttcttatcactgaaggaaccatgatctctccctcttctgcgggctttccacatgtaccgggcatttttactaaagagcaagtcgaaggttggaagaaagtcgtggatgcggcccacaaagagggagctgtgatcttctgtcaactctggcatgtcggtcgtgcctctcatcaagtttatcagccgggcggggctgcacctatcagtagcacctcaaaaccaattagtaaaaaatgggaaattctgttacctgatgcaacatatggcacgtacccggaaccgcgtcctctggctgccaacgaaattttagaggttgtcgaagattatcgcgtggcggccattaacgcgatcgaagcgggctttgacggcatcgaaatccatggagcgcatggctatctgcttgatcaattcatgaaagatggtatcaatgatcgtactgatgaatacggaggctctctggaaaaccgctgtaaattcatccttcaagtcgtacaggctgtcagcgcagcaatcggaacggatcgtgtcggtatccgtatcagcccggcaattgaccataccgacgccatggacagcgatccgcgctccttaggtctggctgttattgagcgcctgaataaacttcagttcaaactgggttcccgcttagcgtatttacatgttacgcaaccacgttatactgccgatggccacggccagacagaagccggggcaaacggctctgaagaggaagtagcgcaactgatgaagacgtggcgcggtgcgtacgtgggcaccttcatctgttgtggcggttatactcgcgagttaggactccaagcggtggctcaaggggacgctgatctggtcgccttcggacgttactttgtaagtaaccctgacttggtactgcgcttaaagcttaatgcgccgctgaaccgctacgatcgtgcaaccttttacactcacgatccggtggtcggctacacggattatccttccctggacaaaggtagtcttttataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K823005_sequence 1 tttacagctagctcagtcctaggtattatgctagc BBa_S05305_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgtctgaaaaaccaacattatttagcgcgtataaaatggggaaatttaatctgtcgcaccgcgtggtgctggcgccaatgacccgttgccgcgccattaacgccattccgaatgaagccctggtggaatattatcgccaacgcagtaccgcaggtggctttcttatcactgaaggaaccatgatctctccctcttctgcgggctttccacatgtaccgggcatttttactaaagagcaagtcgaaggttggaagaaagtcgtggatgcggcccacaaagagggagctgtgatcttctgtcaactctggcatgtcggtcgtgcctctcatcaagtttatcagccgggcggggctgcacctatcagtagcacctcaaaaccaattagtaaaaaatgggaaattctgttacctgatgcaacatatggcacgtacccggaaccgcgtcctctggctgccaacgaaattttagaggttgtcgaagattatcgcgtggcggccattaacgcgatcgaagcgggctttgacggcatcgaaatccatggagcgcatggctatctgcttgatcaattcatgaaagatggtatcaatgatcgtactgatgaatacggaggctctctggaaaaccgctgtaaattcatccttcaagtcgtacaggctgtcagcgcagcaatcggaacggatcgtgtcggtatccgtatcagcccggcaattgaccataccgacgccatggacagcgatccgcgctccttaggtctggctgttattgagcgcctgaataaacttcagttcaaactgggttcccgcttagcgtatttacatgttacgcaaccacgttatactgccgatggccacggccagacagaagccggggcaaacggctctgaagaggaagtagcgcaactgatgaagacgtggcgcggtgcgtacgtgggcaccttcatctgttgtggcggttatactcgcgagttaggactccaagcggtggctcaaggggacgctgatctggtcgccttcggacgttactttgtaagtaaccctgacttggtactgcgcttaaagcttaatgcgccgctgaaccgctacgatcgtgcaaccttttacactcacgatccggtggtcggctacacggattatccttccctggacaaaggtagtcttttataa BBa_S05306_sequence 1 tcacacaggaaagtactagatgtcctcaggggcgaatggctcgagtaagtctgcatcacataaaatcaaattcactaaactgtttattaacggtgaatttgtagattcgatcagtggtaacaccttcgataccatcaaccctgcgactgaagaagtgctggcaacagttgcggagggacgcaaagaagatattgacctggctgtgaaagctgcgcgcgaggcctttgataacggtccctggccgcggatgtctggggaggcgcggcgcaagatcatgctgaaatttgccgatttaattgacgaaaacgccgacgaactgacgacacttgaagtaattgatggcgggaagttgtttggtcctgtgcgtcactttgaggttccggtgtctagcgatacttttcgttacttcgccggtgcagcagataaaattcgtggggcgacgctgaaaatgtcttcgaatatccaggcgtacactttacgcgagccgattggcgtagtaggtcacatcatcccgtggaacggtccagccttcatgtttgcaaccaaggtagctccggcactcgcggcgggatgcaccatggtgattaagccagcggaacacacaccactgacggtgctgtttctggcgcacctgagcaaactcgcgggagttccggacggtgttatcaacgttgtcaacgggtttggcaaaacggctggcgcggcggttagctcacatatggacatcgacatggtgaccttcaccggatcaaccgaggtgggtcgtacggtgatgcaggctgccgctctgagcaacctgaagccggtatcgctggagctggggggcaaaagcccgctgatcgtttttgacgatgccgatgtggacaaagctgccgaatttgccatcttgggtaattttaccaacaaaggagaaatgtgtgttgctggaagccgtgtttttgtgcaggagggcatccatgatgtcttcgtgaaaaagctggaaggggctgttaaagcctgggcgacacgtgacccattcgacctggccacccgtcatggcccgcaaaacaataaacagcaatatgacaaagtactcagttgtatcaatcatggaaaaaaagaaggggcgaccctggttacgggcggcaagccctttgggaaaaaaggctattatatcgaacctaccctgttcacgaatgtcaccgatgacatgaccatcgcgaaagaagaaatctttgggcctgtcattagcgtgttaaaattcaaaacagtggaggaggttattaaacgtgccaacgcgacgaagtatggtctcgcgtcaggcgtgttcactaagaatattgatgtagtgaatacggtgagccgttcaatccgtgcgggtgcggtttgggtcaattgttatttagccttggaccgggacgcacctcatggtgggtataaaatgtcggggttcggccgcgaacaggggttagaagcgctggaacattatttacagattaaaaccgtggcaacgccaatctacgatagcccatggctccatcaccatcaccatcactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_S05303_sequence 1 tcacacaggaaagtactagatgtcctcaggggcgaatggctcgagtaagtctgcatcacataaaatcaaattcactaaactgtttattaacggtgaatttgtagattcgatcagtggtaacaccttcgataccatcaaccctgcgactgaagaagtgctggcaacagttgcggagggacgcaaagaagatattgacctggctgtgaaagctgcgcgcgaggcctttgataacggtccctggccgcggatgtctggggaggcgcggcgcaagatcatgctgaaatttgccgatttaattgacgaaaacgccgacgaactgacgacacttgaagtaattgatggcgggaagttgtttggtcctgtgcgtcactttgaggttccggtgtctagcgatacttttcgttacttcgccggtgcagcagataaaattcgtggggcgacgctgaaaatgtcttcgaatatccaggcgtacactttacgcgagccgattggcgtagtaggtcacatcatcccgtggaacggtccagccttcatgtttgcaaccaaggtagctccggcactcgcggcgggatgcaccatggtgattaagccagcggaacacacaccactgacggtgctgtttctggcgcacctgagcaaactcgcgggagttccggacggtgttatcaacgttgtcaacgggtttggcaaaacggctggcgcggcggttagctcacatatggacatcgacatggtgaccttcaccggatcaaccgaggtgggtcgtacggtgatgcaggctgccgctctgagcaacctgaagccggtatcgctggagctggggggcaaaagcccgctgatcgtttttgacgatgccgatgtggacaaagctgccgaatttgccatcttgggtaattttaccaacaaaggagaaatgtgtgttgctggaagccgtgtttttgtgcaggagggcatccatgatgtcttcgtgaaaaagctggaaggggctgttaaagcctgggcgacacgtgacccattcgacctggccacccgtcatggcccgcaaaacaataaacagcaatatgacaaagtactcagttgtatcaatcatggaaaaaaagaaggggcgaccctggttacgggcggcaagccctttgggaaaaaaggctattatatcgaacctaccctgttcacgaatgtcaccgatgacatgaccatcgcgaaagaagaaatctttgggcctgtcattagcgtgttaaaattcaaaacagtggaggaggttattaaacgtgccaacgcgacgaagtatggtctcgcgtcaggcgtgttcactaagaatattgatgtagtgaatacggtgagccgttcaatccgtgcgggtgcggtttgggtcaattgttatttagccttggaccgggacgcacctcatggtgggtataaaatgtcggggttcggccgcgaacaggggttagaagcgctggaacattatttacagattaaaaccgtggcaacgccaatctacgatagcccatggctccatcaccatcaccatcactaa BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1808012_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgtctgaaaaaccaacattatttagcgcgtataaaatggggaaatttaatctgtcgcaccgcgtggtgctggcgccaatgacccgttgccgcgccattaacgccattccgaatgaagccctggtggaatattatcgccaacgcagtaccgcaggtggctttcttatcactgaaggaaccatgatctctccctcttctgcgggctttccacatgtaccgggcatttttactaaagagcaagtcgaaggttggaagaaagtcgtggatgcggcccacaaagagggagctgtgatcttctgtcaactctggcatgtcggtcgtgcctctcatcaagtttatcagccgggcggggctgcacctatcagtagcacctcaaaaccaattagtaaaaaatgggaaattctgttacctgatgcaacatatggcacgtacccggaaccgcgtcctctggctgccaacgaaattttagaggttgtcgaagattatcgcgtggcggccattaacgcgatcgaagcgggctttgacggcatcgaaatccatggagcgcatggctatctgcttgatcaattcatgaaagatggtatcaatgatcgtactgatgaatacggaggctctctggaaaaccgctgtaaattcatccttcaagtcgtacaggctgtcagcgcagcaatcggaacggatcgtgtcggtatccgtatcagcccggcaattgaccataccgacgccatggacagcgatccgcgctccttaggtctggctgttattgagcgcctgaataaacttcagttcaaactgggttcccgcttagcgtatttacatgttacgcaaccacgttatactgccgatggccacggccagacagaagccggggcaaacggctctgaagaggaagtagcgcaactgatgaagacgtggcgcggtgcgtacgtgggcaccttcatctgttgtggcggttatactcgcgagttaggactccaagcggtggctcaaggggacgctgatctggtcgccttcggacgttactttgtaagtaaccctgacttggtactgcgcttaaagcttaatgcgccgctgaaccgctacgatcgtgcaaccttttacactcacgatccggtggtcggctacacggattatccttccctggacaaaggtagtcttttataatactagagtcacacaggaaagtactagatgtcctcaggggcgaatggctcgagtaagtctgcatcacataaaatcaaattcactaaactgtttattaacggtgaatttgtagattcgatcagtggtaacaccttcgataccatcaaccctgcgactgaagaagtgctggcaacagttgcggagggacgcaaagaagatattgacctggctgtgaaagctgcgcgcgaggcctttgataacggtccctggccgcggatgtctggggaggcgcggcgcaagatcatgctgaaatttgccgatttaattgacgaaaacgccgacgaactgacgacacttgaagtaattgatggcgggaagttgtttggtcctgtgcgtcactttgaggttccggtgtctagcgatacttttcgttacttcgccggtgcagcagataaaattcgtggggcgacgctgaaaatgtcttcgaatatccaggcgtacactttacgcgagccgattggcgtagtaggtcacatcatcccgtggaacggtccagccttcatgtttgcaaccaaggtagctccggcactcgcggcgggatgcaccatggtgattaagccagcggaacacacaccactgacggtgctgtttctggcgcacctgagcaaactcgcgggagttccggacggtgttatcaacgttgtcaacgggtttggcaaaacggctggcgcggcggttagctcacatatggacatcgacatggtgaccttcaccggatcaaccgaggtgggtcgtacggtgatgcaggctgccgctctgagcaacctgaagccggtatcgctggagctggggggcaaaagcccgctgatcgtttttgacgatgccgatgtggacaaagctgccgaatttgccatcttgggtaattttaccaacaaaggagaaatgtgtgttgctggaagccgtgtttttgtgcaggagggcatccatgatgtcttcgtgaaaaagctggaaggggctgttaaagcctgggcgacacgtgacccattcgacctggccacccgtcatggcccgcaaaacaataaacagcaatatgacaaagtactcagttgtatcaatcatggaaaaaaagaaggggcgaccctggttacgggcggcaagccctttgggaaaaaaggctattatatcgaacctaccctgttcacgaatgtcaccgatgacatgaccatcgcgaaagaagaaatctttgggcctgtcattagcgtgttaaaattcaaaacagtggaggaggttattaaacgtgccaacgcgacgaagtatggtctcgcgtcaggcgtgttcactaagaatattgatgtagtgaatacggtgagccgttcaatccgtgcgggtgcggtttgggtcaattgttatttagccttggaccgggacgcacctcatggtgggtataaaatgtcggggttcggccgcgaacaggggttagaagcgctggaacattatttacagattaaaaccgtggcaacgccaatctacgatagcccatggctccatcaccatcaccatcactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0032_sequence 1 tcacacaggaaag BBa_K1808000_sequence 1 atgtctgaaaaaccaacattatttagcgcgtataaaatggggaaatttaatctgtcgcaccgcgtggtgctggcgccaatgacccgttgccgcgccattaacgccattccgaatgaagccctggtggaatattatcgccaacgcagtaccgcaggtggctttcttatcactgaaggaaccatgatctctccctcttctgcgggctttccacatgtaccgggcatttttactaaagagcaagtcgaaggttggaagaaagtcgtggatgcggcccacaaagagggagctgtgatcttctgtcaactctggcatgtcggtcgtgcctctcatcaagtttatcagccgggcggggctgcacctatcagtagcacctcaaaaccaattagtaaaaaatgggaaattctgttacctgatgcaacatatggcacgtacccggaaccgcgtcctctggctgccaacgaaattttagaggttgtcgaagattatcgcgtggcggccattaacgcgatcgaagcgggctttgacggcatcgaaatccatggagcgcatggctatctgcttgatcaattcatgaaagatggtatcaatgatcgtactgatgaatacggaggctctctggaaaaccgctgtaaattcatccttcaagtcgtacaggctgtcagcgcagcaatcggaacggatcgtgtcggtatccgtatcagcccggcaattgaccataccgacgccatggacagcgatccgcgctccttaggtctggctgttattgagcgcctgaataaacttcagttcaaactgggttcccgcttagcgtatttacatgttacgcaaccacgttatactgccgatggccacggccagacagaagccggggcaaacggctctgaagaggaagtagcgcaactgatgaagacgtggcgcggtgcgtacgtgggcaccttcatctgttgtggcggttatactcgcgagttaggactccaagcggtggctcaaggggacgctgatctggtcgccttcggacgttactttgtaagtaaccctgacttggtactgcgcttaaagcttaatgcgccgctgaaccgctacgatcgtgcaaccttttacactcacgatccggtggtcggctacacggattatccttccctggacaaaggtagtcttttataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1808004_sequence 1 atgtcctcaggggcgaatggctcgagtaagtctgcatcacataaaatcaaattcactaaactgtttattaacggtgaatttgtagattcgatcagtggtaacaccttcgataccatcaaccctgcgactgaagaagtgctggcaacagttgcggagggacgcaaagaagatattgacctggctgtgaaagctgcgcgcgaggcctttgataacggtccctggccgcggatgtctggggaggcgcggcgcaagatcatgctgaaatttgccgatttaattgacgaaaacgccgacgaactgacgacacttgaagtaattgatggcgggaagttgtttggtcctgtgcgtcactttgaggttccggtgtctagcgatacttttcgttacttcgccggtgcagcagataaaattcgtggggcgacgctgaaaatgtcttcgaatatccaggcgtacactttacgcgagccgattggcgtagtaggtcacatcatcccgtggaacggtccagccttcatgtttgcaaccaaggtagctccggcactcgcggcgggatgcaccatggtgattaagccagcggaacacacaccactgacggtgctgtttctggcgcacctgagcaaactcgcgggagttccggacggtgttatcaacgttgtcaacgggtttggcaaaacggctggcgcggcggttagctcacatatggacatcgacatggtgaccttcaccggatcaaccgaggtgggtcgtacggtgatgcaggctgccgctctgagcaacctgaagccggtatcgctggagctggggggcaaaagcccgctgatcgtttttgacgatgccgatgtggacaaagctgccgaatttgccatcttgggtaattttaccaacaaaggagaaatgtgtgttgctggaagccgtgtttttgtgcaggagggcatccatgatgtcttcgtgaaaaagctggaaggggctgttaaagcctgggcgacacgtgacccattcgacctggccacccgtcatggcccgcaaaacaataaacagcaatatgacaaagtactcagttgtatcaatcatggaaaaaaagaaggggcgaccctggttacgggcggcaagccctttgggaaaaaaggctattatatcgaacctaccctgttcacgaatgtcaccgatgacatgaccatcgcgaaagaagaaatctttgggcctgtcattagcgtgttaaaattcaaaacagtggaggaggttattaaacgtgccaacgcgacgaagtatggtctcgcgtcaggcgtgttcactaagaatattgatgtagtgaatacggtgagccgttcaatccgtgcgggtgcggtttgggtcaattgttatttagccttggaccgggacgcacctcatggtgggtataaaatgtcggggttcggccgcgaacaggggttagaagcgctggaacattatttacagattaaaaccgtggcaacgccaatctacgatagcccatggctccatcaccatcaccatcactaa BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z