BBa_K1808012
type: ComponentDefinition
persistent identity: BBa K1808012
display id: BBa_K1808012
version: 1
was generated by: iGEM to SBOL conversion
description: DBR2 and ALDH1 generator "Alpha"
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T12:20:28Z
mutable provenance: Artemisia annua
owned by: james
owned by: myers
mutable description: The Alpha construct
discontinued: false
dominant: false
group u list: _2233_
m user id: 26113
owner id: 26113
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This part is BioBrick 3A assembly, E. coli codon and synthesis optimised. This part is partially 6 His tagged to allow purification of ALDH1 protein.
star: false
creator: Arnas Petrauskas
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_S05305
component: BBa_S05306
sequence annotation: BBa_S05305
sequence annotation: BBa_S05306
sequence: https://synbiohub.org/public/igem/BBa_K1808012_sequence/1

BBa_S05305
type: Component
persistent identity: component2461599
display id: component2461599
version: 1
top level: BBa_K1808012
definition: BBa_S05305
access: public

BBa_S05306
type: Component
persistent identity: component2461614
display id: component2461614
version: 1
top level: BBa_K1808012
definition: BBa_S05306
access: public

BBa_S05305
type: SequenceAnnotation
persistent identity: annotation2461599
display id: annotation2461599
version: 1
top level: BBa_K1808012
location: https://synbiohub.org/public/igem/BBa_K1808012/annotation2461599/range2461599/1
component: BBa_S05305

https://synbiohub.org/public/igem/BBa_K1808012/annotation2461599/range2461599/1
type: Range
persistent identity: range2461599
display id: range2461599
version: 1
top level: BBa_K1808012
direction: direction/reverse
start: 1
end: 1225
orientation: inline

BBa_S05306
type: SequenceAnnotation
persistent identity: annotation2461614
display id: annotation2461614
version: 1
top level: BBa_K1808012
location: https://synbiohub.org/public/igem/BBa_K1808012/annotation2461614/range2461614/1
component: BBa_S05306

https://synbiohub.org/public/igem/BBa_K1808012/annotation2461614/range2461614/1
type: Range
persistent identity: range2461614
display id: range2461614
version: 1
top level: BBa_K1808012
direction: direction/reverse
start: 1234
end: 2907
orientation: inline

BBa_S05305
type: ComponentDefinition
persistent identity: BBa S05305
display id: BBa_S05305
version: 1
was generated by: iGEM to SBOL conversion
description: K823005:S05304
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T11:46:21Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 26124
owner id: 26124
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Remsha Afzal
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K823005
component: BBa_S05304
sequence annotation: BBa_K823005
sequence annotation: BBa_S05304
sequence: https://synbiohub.org/public/igem/BBa_S05305_sequence/1

BBa_K823005
type: Component
persistent identity: component2461446
display id: component2461446
version: 1
top level: BBa_S05305
definition: BBa_K823005
access: public

BBa_S05304
type: Component
persistent identity: component2461451
display id: component2461451
version: 1
top level: BBa_S05305
definition: BBa_S05304
access: public

BBa_K823005
type: SequenceAnnotation
persistent identity: annotation2461446
display id: annotation2461446
version: 1
top level: BBa_S05305
location: https://synbiohub.org/public/igem/BBa_S05305/annotation2461446/range2461446/1
component: BBa_K823005

https://synbiohub.org/public/igem/BBa_S05305/annotation2461446/range2461446/1
type: Range
persistent identity: range2461446
display id: range2461446
version: 1
top level: BBa_S05305
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_S05304
type: SequenceAnnotation
persistent identity: annotation2461451
display id: annotation2461451
version: 1
top level: BBa_S05305
location: https://synbiohub.org/public/igem/BBa_S05305/annotation2461451/range2461451/1
component: BBa_S05304

https://synbiohub.org/public/igem/BBa_S05305/annotation2461451/range2461451/1
type: Range
persistent identity: range2461451
display id: range2461451
version: 1
top level: BBa_S05305
direction: direction/reverse
start: 44
end: 1225
orientation: inline

BBa_S05306
type: ComponentDefinition
persistent identity: BBa S05306
display id: BBa_S05306
version: 1
was generated by: iGEM to SBOL conversion
description: S05303:B0015
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T11:47:49Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 26113
owner id: 26113
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Arnas Petrauskas
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_S05303
component: BBa_B0015
sequence annotation: BBa_S05303
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_S05306_sequence/1

BBa_S05303
type: Component
persistent identity: component2461458
display id: component2461458
version: 1
top level: BBa_S05306
definition: BBa_S05303
access: public

BBa_B0015
type: Component
persistent identity: component2461465
display id: component2461465
version: 1
top level: BBa_S05306
definition: BBa_B0015
access: public

BBa_S05303
type: SequenceAnnotation
persistent identity: annotation2461458
display id: annotation2461458
version: 1
top level: BBa_S05306
location: https://synbiohub.org/public/igem/BBa_S05306/annotation2461458/range2461458/1
component: BBa_S05303

https://synbiohub.org/public/igem/BBa_S05306/annotation2461458/range2461458/1
type: Range
persistent identity: range2461458
display id: range2461458
version: 1
top level: BBa_S05306
direction: direction/reverse
start: 1
end: 1537
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2461465
display id: annotation2461465
version: 1
top level: BBa_S05306
location: https://synbiohub.org/public/igem/BBa_S05306/annotation2461465/range2461465/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_S05306/annotation2461465/range2461465/1
type: Range
persistent identity: range2461465
display id: range2461465
version: 1
top level: BBa_S05306
direction: direction/reverse
start: 1546
end: 1674
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_S05303
type: ComponentDefinition
persistent identity: BBa S05303
display id: BBa_S05303
version: 1
was generated by: iGEM to SBOL conversion
description: B0032:K1808004
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T11:34:09Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 26125
owner id: 26125
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: D??ire Gannon
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0032
component: BBa_K1808004
sequence annotation: BBa_B0032
sequence annotation: BBa_K1808004
sequence: https://synbiohub.org/public/igem/BBa_S05303_sequence/1

BBa_B0032
type: Component
persistent identity: component2461382
display id: component2461382
version: 1
top level: BBa_S05303
definition: BBa_B0032
access: public

BBa_K1808004
type: Component
persistent identity: component2461386
display id: component2461386
version: 1
top level: BBa_S05303
definition: BBa_K1808004
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2461382
display id: annotation2461382
version: 1
top level: BBa_S05303
location: https://synbiohub.org/public/igem/BBa_S05303/annotation2461382/range2461382/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_S05303/annotation2461382/range2461382/1
type: Range
persistent identity: range2461382
display id: range2461382
version: 1
top level: BBa_S05303
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_K1808004
type: SequenceAnnotation
persistent identity: annotation2461386
display id: annotation2461386
version: 1
top level: BBa_S05303
location: https://synbiohub.org/public/igem/BBa_S05303/annotation2461386/range2461386/1
component: BBa_K1808004

https://synbiohub.org/public/igem/BBa_S05303/annotation2461386/range2461386/1
type: Range
persistent identity: range2461386
display id: range2461386
version: 1
top level: BBa_S05303
direction: direction/reverse
start: 20
end: 1537
orientation: inline

BBa_S05304
type: ComponentDefinition
persistent identity: BBa S05304
display id: BBa_S05304
version: 1
was generated by: iGEM to SBOL conversion
description: B0034:K1808000
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T11:43:55Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 26125
owner id: 26125
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: D??ire Gannon
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_K1808000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1808000
sequence: https://synbiohub.org/public/igem/BBa_S05304_sequence/1

BBa_B0034
type: Component
persistent identity: component2461428
display id: component2461428
version: 1
top level: BBa_S05304
definition: BBa_B0034
access: public

BBa_K1808000
type: Component
persistent identity: component2461430
display id: component2461430
version: 1
top level: BBa_S05304
definition: BBa_K1808000
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2461428
display id: annotation2461428
version: 1
top level: BBa_S05304
location: https://synbiohub.org/public/igem/BBa_S05304/annotation2461428/range2461428/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S05304/annotation2461428/range2461428/1
type: Range
persistent identity: range2461428
display id: range2461428
version: 1
top level: BBa_S05304
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1808000
type: SequenceAnnotation
persistent identity: annotation2461430
display id: annotation2461430
version: 1
top level: BBa_S05304
location: https://synbiohub.org/public/igem/BBa_S05304/annotation2461430/range2461430/1
component: BBa_K1808000

https://synbiohub.org/public/igem/BBa_S05304/annotation2461430/range2461430/1
type: Range
persistent identity: range2461430
display id: range2461430
version: 1
top level: BBa_S05304
direction: direction/reverse
start: 19
end: 1182
orientation: inline

BBa_K823005
type: ComponentDefinition
persistent identity: BBa K823005
display id: BBa_K823005
version: 1
was generated by: iGEM to SBOL conversion
description: Anderson promoter J23101
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:44:25Z
mutable provenance: Partsregistry
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Anderson promoter J23101 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations
star: false
creator: Korinna Kraft
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23101
sequence annotation: -35 box
sequence annotation: -10 box
sequence: https://synbiohub.org/public/igem/BBa_K823005_sequence/1

J23101
type: SequenceAnnotation
persistent identity: annotation2182509
display id: annotation2182509
version: 1
top level: BBa_K823005
location: https://synbiohub.org/public/igem/BBa_K823005/annotation2182509/range2182509/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K823005/annotation2182509/range2182509/1
type: Range
persistent identity: range2182509
display id: range2182509
version: 1
top level: BBa_K823005
direction: direction/forward
start: 1
end: 35
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182510
display id: annotation2182510
version: 1
top level: BBa_K823005
location: https://synbiohub.org/public/igem/BBa_K823005/annotation2182510/range2182510/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K823005/annotation2182510/range2182510/1
type: Range
persistent identity: range2182510
display id: range2182510
version: 1
top level: BBa_K823005
direction: direction/forward
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182511
display id: annotation2182511
version: 1
top level: BBa_K823005
location: https://synbiohub.org/public/igem/BBa_K823005/annotation2182511/range2182511/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K823005/annotation2182511/range2182511/1
type: Range
persistent identity: range2182511
display id: range2182511
version: 1
top level: BBa_K823005
direction: direction/forward
start: 24
end: 29
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K1808000
type: ComponentDefinition
persistent identity: BBa K1808000
display id: BBa_K1808000
version: 1
was generated by: iGEM to SBOL conversion
description: DBR2 gene from Artemisia annua
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T08:55:06Z
mutable provenance: Artemissia annua
owned by: james
owned by: myers
mutable description: This is the coding region of the Dbr2 gene from Artemisia annua with added biobrick suffix and prefix in a pSB1C3 plasmid backbone. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The Dbr2 gene encodes the enzyme artemisinic aldehyde delta11(13)-reductase, which catalyses the conversion of artemisinic aldehyde to (11R)-dihydroartemisinic aldehyde with NADPH as the electron donor substrate. The product can be acted upon by the Artemisia annua aldehyde dehydrogenase 1 enzyme (see part BBa_K1808001) to give rise to dihydroartemisinic acid, which can be isolated and converted to the anti-malarial artemisinin. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells.
discontinued: false
dominant: false
group u list: _2233_
m user id: 26113
owner id: 26113
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This part is BioBrick 3A assembly, E. coli codon and synthesis optimised.
star: false
creator: Arnas Petrauskas
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: DBR2
sequence: https://synbiohub.org/public/igem/BBa_K1808000_sequence/1

DBR2
type: SequenceAnnotation
persistent identity: annotation2460888
display id: annotation2460888
version: 1
top level: BBa_K1808000
location: https://synbiohub.org/public/igem/BBa_K1808000/annotation2460888/range2460888/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1808000/annotation2460888/range2460888/1
type: Range
persistent identity: range2460888
display id: range2460888
version: 1
top level: BBa_K1808000
direction: direction/forward
start: 1
end: 1164
orientation: inline

BBa_K1808004
type: ComponentDefinition
persistent identity: BBa K1808004
display id: BBa_K1808004
version: 1
was generated by: iGEM to SBOL conversion
description: ALDH1 from Artemisia annua, 6 His tagged
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T06:12:06Z
mutable provenance: Artemisia annua
owned by: james
owned by: myers
mutable description: This is the coding region of the ALDH1 gene from Artemisia annua with added biobrick suffix, prefix and a 6 His tag in a pSB1C3 plasmid backbone. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The ALDH1 gene encodes the enzyme aldehyde dehydrogenase 1, which catalyses the conversion of (11R)-dihydroartemisinic aldehyde to dihydroartemisinic acid as well as the conversion of artemisinic aldehyde to artemisinic acid with NADP+ as the substrate. Artemisinic acid and dihydroartemisinic acid can be extracted from E. coli cells through lysis and chemically converted to the anti-malarial artemisinin. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2233_
m user id: 26113
owner id: 26113
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: This part is BioBrick 3A assembly, E. coli codon and synthesis optimised. This part is 6 His tagged to allow protein purification.
star: false
creator: Arnas Petrauskas
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ALDH1 6 His tagged
sequence annotation: 6 His
sequence: https://synbiohub.org/public/igem/BBa_K1808004_sequence/1

ALDH1 6 His tagged
type: SequenceAnnotation
persistent identity: annotation2461243
display id: annotation2461243
version: 1
top level: BBa_K1808004
location: https://synbiohub.org/public/igem/BBa_K1808004/annotation2461243/range2461243/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1808004/annotation2461243/range2461243/1
type: Range
persistent identity: range2461243
display id: range2461243
version: 1
top level: BBa_K1808004
direction: direction/forward
start: 1
end: 1518
orientation: inline

6 His
type: SequenceAnnotation
persistent identity: annotation2461246
display id: annotation2461246
version: 1
top level: BBa_K1808004
location: https://synbiohub.org/public/igem/BBa_K1808004/annotation2461246/range2461246/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K1808004/annotation2461246/range2461246/1
type: Range
persistent identity: range2461246
display id: range2461246
version: 1
top level: BBa_K1808004
direction: direction/forward
start: 1497
end: 1515
orientation: inline

https://synbiohub.org/public/igem/BBa_K1808012_sequence/1
type: Sequence
persistent identity: BBa K1808012 sequence
display id: BBa_K1808012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgtctgaaaaaccaacattatttagcgcgtataaaatggggaaatttaatctgtcgcaccgcgtggtgctggcgccaatgacccgttgccgcgccattaacgccattccgaatgaagccctggtggaatattatcgccaacgcagtaccgcaggtggctttcttatcactgaaggaaccatgatctctccctcttctgcgggctttccacatgtaccgggcatttttactaaagagcaagtcgaaggttggaagaaagtcgtggatgcggcccacaaagagggagctgtgatcttctgtcaactctggcatgtcggtcgtgcctctcatcaagtttatcagccgggcggggctgcacctatcagtagcacctcaaaaccaattagtaaaaaatgggaaattctgttacctgatgcaacatatggcacgtacccggaaccgcgtcctctggctgccaacgaaattttagaggttgtcgaagattatcgcgtggcggccattaacgcgatcgaagcgggctttgacggcatcgaaatccatggagcgcatggctatctgcttgatcaattcatgaaagatggtatcaatgatcgtactgatgaatacggaggctctctggaaaaccgctgtaaattcatccttcaagtcgtacaggctgtcagcgcagcaatcggaacggatcgtgtcggtatccgtatcagcccggcaattgaccataccgacgccatggacagcgatccgcgctccttaggtctggctgttattgagcgcctgaataaacttcagttcaaactgggttcccgcttagcgtatttacatgttacgcaaccacgttatactgccgatggccacggccagacagaagccggggcaaacggctctgaagaggaagtagcgcaactgatgaagacgtggcgcggtgcgtacgtgggcaccttcatctgttgtggcggttatactcgcgagttaggactccaagcggtggctcaaggggacgctgatctggtcgccttcggacgttactttgtaagtaaccctgacttggtactgcgcttaaagcttaatgcgccgctgaaccgctacgatcgtgcaaccttttacactcacgatccggtggtcggctacacggattatccttccctggacaaaggtagtcttttataatactagagtcacacaggaaagtactagatgtcctcaggggcgaatggctcgagtaagtctgcatcacataaaatcaaattcactaaactgtttattaacggtgaatttgtagattcgatcagtggtaacaccttcgataccatcaaccctgcgactgaagaagtgctggcaacagttgcggagggacgcaaagaagatattgacctggctgtgaaagctgcgcgcgaggcctttgataacggtccctggccgcggatgtctggggaggcgcggcgcaagatcatgctgaaatttgccgatttaattgacgaaaacgccgacgaactgacgacacttgaagtaattgatggcgggaagttgtttggtcctgtgcgtcactttgaggttccggtgtctagcgatacttttcgttacttcgccggtgcagcagataaaattcgtggggcgacgctgaaaatgtcttcgaatatccaggcgtacactttacgcgagccgattggcgtagtaggtcacatcatcccgtggaacggtccagccttcatgtttgcaaccaaggtagctccggcactcgcggcgggatgcaccatggtgattaagccagcggaacacacaccactgacggtgctgtttctggcgcacctgagcaaactcgcgggagttccggacggtgttatcaacgttgtcaacgggtttggcaaaacggctggcgcggcggttagctcacatatggacatcgacatggtgaccttcaccggatcaaccgaggtgggtcgta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05305_sequence/1
type: Sequence
persistent identity: BBa S05305 sequence
display id: BBa_S05305_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgtctgaaaaaccaacattatttagcgcgtataaaatggggaaatttaatctgtcgcaccgcgtggtgctggcgccaatgacccgttgccgcgccattaacgccattccgaatgaagccctggtggaatattatcgccaacgcagtaccgcaggtggctttcttatcactgaaggaaccatgatctctccctcttctgcgggctttccacatgtaccgggcatttttactaaagagcaagtcgaaggttggaagaaagtcgtggatgcggcccacaaagagggagctgtgatcttctgtcaactctggcatgtcggtcgtgcctctcatcaagtttatcagccgggcggggctgcacctatcagtagcacctcaaaaccaattagtaaaaaatgggaaattctgttacctgatgcaacatatggcacgtacccggaaccgcgtcctctggctgccaacgaaattttagaggttgtcgaagattatcgcgtggcggccattaacgcgatcgaagcgggctttgacggcatcgaaatccatggagcgcatggctatctgcttgatcaattcatgaaagatggtatcaatgatcgtactgatgaatacggaggctctctggaaaaccgctgtaaattcatccttcaagtcgtacaggctgtcagcgcagcaatcggaacggatcgtgtcggtatccgtatcagcccggcaattgaccataccgacgccatggacagcgatccgcgctccttaggtctggctgttattgagcgcctgaataaacttcagttcaaactgggttcccgcttagcgtatttacatgttacgcaaccacgttatactgccgatggccacggccagacagaagccggggcaaacggctctgaagaggaagtagcgcaactgatgaagacgtggcgcggtgcgtacgtgggcaccttcatctgttgtggcggttatactcgcgagttaggactccaagcggtggctcaaggggacgctgatctggtcgccttcggacgttactttgtaagtaaccctgacttggtactgcgcttaaagcttaatgcgccgctgaaccgctacgatcgtgcaaccttttacactcacgatccggtggtcggctacacggattatccttccctggacaaaggtagtcttttataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05306_sequence/1
type: Sequence
persistent identity: BBa S05306 sequence
display id: BBa_S05306_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgtcctcaggggcgaatggctcgagtaagtctgcatcacataaaatcaaattcactaaactgtttattaacggtgaatttgtagattcgatcagtggtaacaccttcgataccatcaaccctgcgactgaagaagtgctggcaacagttgcggagggacgcaaagaagatattgacctggctgtgaaagctgcgcgcgaggcctttgataacggtccctggccgcggatgtctggggaggcgcggcgcaagatcatgctgaaatttgccgatttaattgacgaaaacgccgacgaactgacgacacttgaagtaattgatggcgggaagttgtttggtcctgtgcgtcactttgaggttccggtgtctagcgatacttttcgttacttcgccggtgcagcagataaaattcgtggggcgacgctgaaaatgtcttcgaatatccaggcgtacactttacgcgagccgattggcgtagtaggtcacatcatcccgtggaacggtccagccttcatgtttgcaaccaaggtagctccggcactcgcggcgggatgcaccatggtgattaagccagcggaacacacaccactgacggtgctgtttctggcgcacctgagcaaactcgcgggagttccggacggtgttatcaacgttgtcaacgggtttggcaaaacggctggcgcggcggttagctcacatatggacatcgacatggtgaccttcaccggatcaaccgaggtgggtcgtacggtgatgcaggctgccgctctgagcaacctgaagccggtatcgctggagctggggggcaaaagcccgctgatcgtttttgacgatgccgatgtggacaaagctgccgaatttgccatcttgggtaattttaccaacaaaggagaaatgtgtgttgctggaagccgtgtttttgtgcaggagggcatccatgatgtcttcgtgaaaaagctggaaggggctgttaaagcctgggcgacacgtgacccattcgacctggccacccgtcatggcccgcaaaacaataaacagcaatatgacaaagtactcagttgtatcaatcatggaaaaaaagaaggggcgaccctggttacgggcggcaagccctttgggaaaaaaggctattatatcgaacctaccctgttcacgaatgtcaccgatgacatgaccatcgcgaaagaagaaatctttgggcctgtcattagcgtgttaaaattcaaaacagtggaggaggttattaaacgtgccaacgcgacgaagtatggtctcgcgtcaggcgtgttcactaagaatattgatgtagtgaatacggtgagccgttcaatccgtgcgggtgcggtttgggtcaattgttatttagccttggaccgggacgcacctcatggtgggtataaaatgtcggggttcggccgcgaacaggggttagaagcgctggaacattatttacagattaaaaccgtggcaacgccaatctacgatagcccatggctccatcaccatcaccatcactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05303_sequence/1
type: Sequence
persistent identity: BBa S05303 sequence
display id: BBa_S05303_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgtcctcaggggcgaatggctcgagtaagtctgcatcacataaaatcaaattcactaaactgtttattaacggtgaatttgtagattcgatcagtggtaacaccttcgataccatcaaccctgcgactgaagaagtgctggcaacagttgcggagggacgcaaagaagatattgacctggctgtgaaagctgcgcgcgaggcctttgataacggtccctggccgcggatgtctggggaggcgcggcgcaagatcatgctgaaatttgccgatttaattgacgaaaacgccgacgaactgacgacacttgaagtaattgatggcgggaagttgtttggtcctgtgcgtcactttgaggttccggtgtctagcgatacttttcgttacttcgccggtgcagcagataaaattcgtggggcgacgctgaaaatgtcttcgaatatccaggcgtacactttacgcgagccgattggcgtagtaggtcacatcatcccgtggaacggtccagccttcatgtttgcaaccaaggtagctccggcactcgcggcgggatgcaccatggtgattaagccagcggaacacacaccactgacggtgctgtttctggcgcacctgagcaaactcgcgggagttccggacggtgttatcaacgttgtcaacgggtttggcaaaacggctggcgcggcggttagctcacatatggacatcgacatggtgaccttcaccggatcaaccgaggtgggtcgtacggtgatgcaggctgccgctctgagcaacctgaagccggtatcgctggagctggggggcaaaagcccgctgatcgtttttgacgatgccgatgtggacaaagctgccgaatttgccatcttgggtaattttaccaacaaaggagaaatgtgtgttgctggaagccgtgtttttgtgcaggagggcatccatgatgtcttcgtgaaaaagctggaaggggctgttaaagcctgggcgacacgtgacccattcgacctggccacccgtcatggcccgcaaaacaataaacagcaatatgacaaagtactcagttgtatcaatcatggaaaaaaagaaggggcgaccctggttacgggcggcaagccctttgggaaaaaaggctattatatcgaacctaccctgttcacgaatgtcaccgatgacatgaccatcgcgaaagaagaaatctttgggcctgtcattagcgtgttaaaattcaaaacagtggaggaggttattaaacgtgccaacgcgacgaagtatggtctcgcgtcaggcgtgttcactaagaatattgatgtagtgaatacggtgagccgttcaatccgtgcgggtgcggtttgggtcaattgttatttagccttggaccgggacgcacctcatggtgggtataaaatgtcggggttcggccgcgaacaggggttagaagcgctggaacattatttacagattaaaaccgtggcaacgccaatctacgatagcccatggctccatcaccatcaccatcactaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05304_sequence/1
type: Sequence
persistent identity: BBa S05304 sequence
display id: BBa_S05304_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtctgaaaaaccaacattatttagcgcgtataaaatggggaaatttaatctgtcgcaccgcgtggtgctggcgccaatgacccgttgccgcgccattaacgccattccgaatgaagccctggtggaatattatcgccaacgcagtaccgcaggtggctttcttatcactgaaggaaccatgatctctccctcttctgcgggctttccacatgtaccgggcatttttactaaagagcaagtcgaaggttggaagaaagtcgtggatgcggcccacaaagagggagctgtgatcttctgtcaactctggcatgtcggtcgtgcctctcatcaagtttatcagccgggcggggctgcacctatcagtagcacctcaaaaccaattagtaaaaaatgggaaattctgttacctgatgcaacatatggcacgtacccggaaccgcgtcctctggctgccaacgaaattttagaggttgtcgaagattatcgcgtggcggccattaacgcgatcgaagcgggctttgacggcatcgaaatccatggagcgcatggctatctgcttgatcaattcatgaaagatggtatcaatgatcgtactgatgaatacggaggctctctggaaaaccgctgtaaattcatccttcaagtcgtacaggctgtcagcgcagcaatcggaacggatcgtgtcggtatccgtatcagcccggcaattgaccataccgacgccatggacagcgatccgcgctccttaggtctggctgttattgagcgcctgaataaacttcagttcaaactgggttcccgcttagcgtatttacatgttacgcaaccacgttatactgccgatggccacggccagacagaagccggggcaaacggctctgaagaggaagtagcgcaactgatgaagacgtggcgcggtgcgtacgtgggcaccttcatctgttgtggcggttatactcgcgagttaggactccaagcggtggctcaaggggacgctgatctggtcgccttcggacgttactttgtaagtaaccctgacttggtactgcgcttaaagcttaatgcgccgctgaaccgctacgatcgtgcaaccttttacactcacgatccggtggtcggctacacggattatccttccctggacaaaggtagtcttttataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K823005_sequence/1
type: Sequence
persistent identity: BBa K823005 sequence
display id: BBa_K823005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1808000_sequence/1
type: Sequence
persistent identity: BBa K1808000 sequence
display id: BBa_K1808000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtctgaaaaaccaacattatttagcgcgtataaaatggggaaatttaatctgtcgcaccgcgtggtgctggcgccaatgacccgttgccgcgccattaacgccattccgaatgaagccctggtggaatattatcgccaacgcagtaccgcaggtggctttcttatcactgaaggaaccatgatctctccctcttctgcgggctttccacatgtaccgggcatttttactaaagagcaagtcgaaggttggaagaaagtcgtggatgcggcccacaaagagggagctgtgatcttctgtcaactctggcatgtcggtcgtgcctctcatcaagtttatcagccgggcggggctgcacctatcagtagcacctcaaaaccaattagtaaaaaatgggaaattctgttacctgatgcaacatatggcacgtacccggaaccgcgtcctctggctgccaacgaaattttagaggttgtcgaagattatcgcgtggcggccattaacgcgatcgaagcgggctttgacggcatcgaaatccatggagcgcatggctatctgcttgatcaattcatgaaagatggtatcaatgatcgtactgatgaatacggaggctctctggaaaaccgctgtaaattcatccttcaagtcgtacaggctgtcagcgcagcaatcggaacggatcgtgtcggtatccgtatcagcccggcaattgaccataccgacgccatggacagcgatccgcgctccttaggtctggctgttattgagcgcctgaataaacttcagttcaaactgggttcccgcttagcgtatttacatgttacgcaaccacgttatactgccgatggccacggccagacagaagccggggcaaacggctctgaagaggaagtagcgcaactgatgaagacgtggcgcggtgcgtacgtgggcaccttcatctgttgtggcggttatactcgcgagttaggactccaagcggtggctcaaggggacgctgatctggtcgccttcggacgttactttgtaagtaaccctgacttggtactgcgcttaaagcttaatgcgccgctgaaccgctacgatcgtgcaaccttttacactcacgatccggtggtcggctacacggattatccttccctggacaaaggtagtcttttataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1808004_sequence/1
type: Sequence
persistent identity: BBa K1808004 sequence
display id: BBa_K1808004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcctcaggggcgaatggctcgagtaagtctgcatcacataaaatcaaattcactaaactgtttattaacggtgaatttgtagattcgatcagtggtaacaccttcgataccatcaaccctgcgactgaagaagtgctggcaacagttgcggagggacgcaaagaagatattgacctggctgtgaaagctgcgcgcgaggcctttgataacggtccctggccgcggatgtctggggaggcgcggcgcaagatcatgctgaaatttgccgatttaattgacgaaaacgccgacgaactgacgacacttgaagtaattgatggcgggaagttgtttggtcctgtgcgtcactttgaggttccggtgtctagcgatacttttcgttacttcgccggtgcagcagataaaattcgtggggcgacgctgaaaatgtcttcgaatatccaggcgtacactttacgcgagccgattggcgtagtaggtcacatcatcccgtggaacggtccagccttcatgtttgcaaccaaggtagctccggcactcgcggcgggatgcaccatggtgattaagccagcggaacacacaccactgacggtgctgtttctggcgcacctgagcaaactcgcgggagttccggacggtgttatcaacgttgtcaacgggtttggcaaaacggctggcgcggcggttagctcacatatggacatcgacatggtgaccttcaccggatcaaccgaggtgggtcgtacggtgatgcaggctgccgctctgagcaacctgaagccggtatcgctggagctggggggcaaaagcccgctgatcgtttttgacgatgccgatgtggacaaagctgccgaatttgccatcttgggtaattttaccaacaaaggagaaatgtgtgttgctggaagccgtgtttttgtgcaggagggcatccatgatgtcttcgtgaaaaagctggaaggggctgttaaagcctgggcgacacgtgacccattcgacctggccacccgtcatggcccgcaaaacaataaacagcaatatgacaaagtactcagttgtatcaatcatggaaaaaaagaaggggcgaccctggttacgggcggcaagccctttgggaaaaaaggctattatatcgaacctaccctgttcacgaatgtcaccgatgacatgaccatcgcgaaagaagaaatctttgggcctgtcattagcgtgttaaaattcaaaacagtggaggaggttattaaacgtgccaacgcgacgaagtatggtctcgcgtcaggcgtgttcactaagaatattgatgtagtgaatacggtgagccgttcaatccgtgcgggtgcggtttgggtcaattgttatttagccttggaccgggacgcacctcatggtgggtataaaatgtcggggttcggccgcgaacaggggttagaagcgctggaacattatttacagattaaaaccgtggcaacgccaatctacgatagcccatggctccatcaccatcaccatcactaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z