LOCUS       BBa_K1809001             729 bp    DNA     linear       22-Nov-2024
DEFINITION  Const. Promoter-RBS-GaAFP-DT
ACCESSION   BBa_K1809001
VERSION     BBa_K1809001.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..592
                     /label=GaAFP
     CDS             62..592
                     /label=BBa_K1809000
     stem_loop       612..655
                     /label=stem_loop
     misc_feature    601..680
                     /label=BBa_B0010
     terminator      601..680
                     /label=BBa_B0010
     stem_loop       696..715
                     /label=T7 TE
     misc_feature    722..722
                     /label=stop
     polyA_site      716..729
                     /label=polya
     misc_feature    689..729
                     /label=BBa_B0012
     terminator      689..729
                     /label=BBa_B0012
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatgcgtagc aattttcacc cgttagccgc cagcttcatt gtgcgttgcg cgtttctgca
      121 ttcacgtcgc ttcaccgatt ccctttttca gttgctgagc tccctgatta gcttgactag
      181 cgcggcgact gccatcgatc tgggcgtggc tggccaatac gacgtcgtgg cgcgcagtgc
      241 tatcaccctg ggcgctttag ccgaaattac gggcaacgta ggtctgagtc cgggactgag
      301 tacggccctg acaggattca cgctggtacc ggtagaagac catggcacgt tttgctccgc
      361 aggagtaaag tattgtggcg cagactccct ttctacttct gcaacgtccc tcttagttaa
      421 aggccgtatc gatgcgcctg actttccctc ctcgccggcc attttagggc aagcagcgac
      481 tgatgtggtt gcggcttgga aatccgcatt ttctcaggaa ctttctcccg ctgactacac
      541 caaacgtgac tttgcaggtg gtcttctgtc tgacctcact ttggcaccag gttactagag
      601 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      661 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      721 gcgtttata
//