BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1809000
1
BBa_K1809000
GaAFP
2015-07-21T11:00:00Z
2016-01-27T10:32:22Z
Composite of parts BBa_J23119, BBa_B0015, BBa_B0034 and BBa_K652004.
Antifreeze protein construct
false
false
_2234_
4206
26152
9
Not in stock
false
Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.
false
Edward Dring
annotation2433988
1
GaAFP
range2433988
1
1
531
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_K1809001
1
BBa_K1809001
Const. Promoter-RBS-GaAFP-DT
2015-07-23T11:00:00Z
2015-08-03T03:50:44Z
Composite of parts BBa_K1423005 and BBa_K652004
This part contains the constitutive promoter BBa_J23119, an RBS, the cell surface expression tag BclA, Zoarces elongatus antifreeze protein (ZeAFP), and a double terminator.
false
false
_2234_
26152
26152
9
false
Spliced together a cell surface tag with the antifreeze protein to test cell surface activity.
false
Edward Dring
component2434063
1
BBa_B0034
component2434061
1
BBa_J23119
component2434066
1
BBa_B0010
component2434068
1
BBa_B0012
component2434065
1
BBa_K1809000
annotation2434066
1
BBa_B0010
range2434066
1
601
680
annotation2434061
1
BBa_J23119
range2434061
1
1
35
annotation2434065
1
BBa_K1809000
range2434065
1
62
592
annotation2434063
1
BBa_B0034
range2434063
1
44
55
annotation2434068
1
BBa_B0012
range2434068
1
689
729
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_K1809000_sequence
1
atgcgtagcaattttcacccgttagccgccagcttcattgtgcgttgcgcgtttctgcattcacgtcgcttcaccgattccctttttcagttgctgagctccctgattagcttgactagcgcggcgactgccatcgatctgggcgtggctggccaatacgacgtcgtggcgcgcagtgctatcaccctgggcgctttagccgaaattacgggcaacgtaggtctgagtccgggactgagtacggccctgacaggattcacgctggtaccggtagaagaccatggcacgttttgctccgcaggagtaaagtattgtggcgcagactccctttctacttctgcaacgtccctcttagttaaaggccgtatcgatgcgcctgactttccctcctcgccggccattttagggcaagcagcgactgatgtggttgcggcttggaaatccgcattttctcaggaactttctcccgctgactacaccaaacgtgactttgcaggtggtcttctgtctgacctcactttggcaccaggt
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1809001_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgcgtagcaattttcacccgttagccgccagcttcattgtgcgttgcgcgtttctgcattcacgtcgcttcaccgattccctttttcagttgctgagctccctgattagcttgactagcgcggcgactgccatcgatctgggcgtggctggccaatacgacgtcgtggcgcgcagtgctatcaccctgggcgctttagccgaaattacgggcaacgtaggtctgagtccgggactgagtacggccctgacaggattcacgctggtaccggtagaagaccatggcacgttttgctccgcaggagtaaagtattgtggcgcagactccctttctacttctgcaacgtccctcttagttaaaggccgtatcgatgcgcctgactttccctcctcgccggccattttagggcaagcagcgactgatgtggttgcggcttggaaatccgcattttctcaggaactttctcccgctgactacaccaaacgtgactttgcaggtggtcttctgtctgacctcactttggcaccaggttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z