BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1809000 1 BBa_K1809000 GaAFP 2015-07-21T11:00:00Z 2016-01-27T10:32:22Z Composite of parts BBa_J23119, BBa_B0015, BBa_B0034 and BBa_K652004. Antifreeze protein construct false false _2234_ 4206 26152 9 Not in stock false Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites. false Edward Dring annotation2433988 1 GaAFP range2433988 1 1 531 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_K1809001 1 BBa_K1809001 Const. Promoter-RBS-GaAFP-DT 2015-07-23T11:00:00Z 2015-08-03T03:50:44Z Composite of parts BBa_K1423005 and BBa_K652004 This part contains the constitutive promoter BBa_J23119, an RBS, the cell surface expression tag BclA, Zoarces elongatus antifreeze protein (ZeAFP), and a double terminator. false false _2234_ 26152 26152 9 false Spliced together a cell surface tag with the antifreeze protein to test cell surface activity. false Edward Dring component2434063 1 BBa_B0034 component2434061 1 BBa_J23119 component2434066 1 BBa_B0010 component2434068 1 BBa_B0012 component2434065 1 BBa_K1809000 annotation2434066 1 BBa_B0010 range2434066 1 601 680 annotation2434061 1 BBa_J23119 range2434061 1 1 35 annotation2434065 1 BBa_K1809000 range2434065 1 62 592 annotation2434063 1 BBa_B0034 range2434063 1 44 55 annotation2434068 1 BBa_B0012 range2434068 1 689 729 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K1809000_sequence 1 atgcgtagcaattttcacccgttagccgccagcttcattgtgcgttgcgcgtttctgcattcacgtcgcttcaccgattccctttttcagttgctgagctccctgattagcttgactagcgcggcgactgccatcgatctgggcgtggctggccaatacgacgtcgtggcgcgcagtgctatcaccctgggcgctttagccgaaattacgggcaacgtaggtctgagtccgggactgagtacggccctgacaggattcacgctggtaccggtagaagaccatggcacgttttgctccgcaggagtaaagtattgtggcgcagactccctttctacttctgcaacgtccctcttagttaaaggccgtatcgatgcgcctgactttccctcctcgccggccattttagggcaagcagcgactgatgtggttgcggcttggaaatccgcattttctcaggaactttctcccgctgactacaccaaacgtgactttgcaggtggtcttctgtctgacctcactttggcaccaggt BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K1809001_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgcgtagcaattttcacccgttagccgccagcttcattgtgcgttgcgcgtttctgcattcacgtcgcttcaccgattccctttttcagttgctgagctccctgattagcttgactagcgcggcgactgccatcgatctgggcgtggctggccaatacgacgtcgtggcgcgcagtgctatcaccctgggcgctttagccgaaattacgggcaacgtaggtctgagtccgggactgagtacggccctgacaggattcacgctggtaccggtagaagaccatggcacgttttgctccgcaggagtaaagtattgtggcgcagactccctttctacttctgcaacgtccctcttagttaaaggccgtatcgatgcgcctgactttccctcctcgccggccattttagggcaagcagcgactgatgtggttgcggcttggaaatccgcattttctcaggaactttctcccgctgactacaccaaacgtgactttgcaggtggtcttctgtctgacctcactttggcaccaggttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z