BBa_K1809003 1 BBa_K1809003 RiAFP 2015-08-02T11:00:00Z 2016-01-27T10:33:32Z Taken from the longhorn beetle Rhagium inquisitor. Pubmed ID: 21078390 Antifreeze proteins (AFPs) are molecules with the unique property of binding and shaping ice crystals, preventing their growth into relatively large cell-lysing structures (Davies and Sykes 1997). They exhibit a property known as thermal hysteresis, whereby their activity decreases the freezing point of water without changing the melting point. This property allows organisms which produce AFPs to survive colder conditions. Additionally, AFPs have shown biolfilm inhibbiting properties, potetnially giving them uses in medical applications. false false _2234_ 4206 26152 9 false Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites. false Edward Dring annotation2433989 1 RiAFP range2433989 1 1 336 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K1423000 1 BBa_K1423000 BCLA N-terminal Domain 2014-09-12T11:00:00Z 2015-05-08T01:10:23Z Bacillus anthracis This part is the N-terminal domain of BCLA, and it can be used as an anchoring motif for cell surface display. BCLA is a hair-like glycoprotein found on the outermost layer of the Bacillus anthracis spore. It consists of three parts: an N terminal domain, a central domain (also called collagen-like region ???CLR???), and a C terminal domain. A picture of the structure of BCLA can be seen below. false false _1801_ 0 22136 9 In stock false When designing the part we ensured that BCLA and any following parts would remain in frame. This is essential because as an anchoring motif we expect additional domains to be attached after. false Shawna Henry BBa_K1809005 1 BBa_K1809005 Const. Promoter-RBS-BclA-RiAFP-DT 2015-08-02T11:00:00Z 2015-08-03T04:04:14Z Composite of parts BBa_K1423005 and BBa_K1809008 without the YFP tag. This part contains the constitutive promoter BBa_J23100, an RBS, the cell surface expression tag BclA, Rhagium inquisitor antifreeze protein (RiAFP), and a double terminator. It encodes an antifreeze protein that attaches to the cell surface. false false _2234_ 26152 26152 9 false Spliced together a cell surface expression tag and the antifreeze protein to test cell surface activity. false Edward Dring component2434092 1 BBa_B0012 component2434086 1 BBa_B0034 component2434089 1 BBa_K1809003 component2434090 1 BBa_B0010 component2434087 1 BBa_K1423000 component2434084 1 BBa_J23100 annotation2434084 1 BBa_J23100 range2434084 1 1 35 annotation2434090 1 BBa_B0010 range2434090 1 477 556 annotation2434092 1 BBa_B0012 range2434092 1 565 605 annotation2434086 1 BBa_B0034 range2434086 1 44 55 annotation2434087 1 BBa_K1423000 range2434087 1 64 126 annotation2434089 1 BBa_K1809003 range2434089 1 133 468 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1423000_sequence 1 gcgttcgatcctaatctggtaggacccacccttccgccgatcccgccattcacactgccaaca BBa_B0034_sequence 1 aaagaggagaaa BBa_K1809005_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagaggcgttcgatcctaatctggtaggacccacccttccgccgatcccgccattcacactgccaacatactagatgcagaaacgccaggtgccgggccaatggctcccgggtcgtctgaacctggatcagcaccagcagccgcaacagcaagatgtgggtcctctgccggaccaactgcgccaggaggaggaacagccgcagcggcagccgcaggtgccaccacaacccttgctgatgccgctggtgcgtgaacagcagcagcagcaacctttggatctgttagtggggggccaacaggatcgccagccccaccatcctgcgcacccgagccaacacaaactgaaaccatctccagatcaggcgttcaaacgccgtaaccatctgcacgagaccttaccacaaccgttgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1809003_sequence 1 atgcagaaacgccaggtgccgggccaatggctcccgggtcgtctgaacctggatcagcaccagcagccgcaacagcaagatgtgggtcctctgccggaccaactgcgccaggaggaggaacagccgcagcggcagccgcaggtgccaccacaacccttgctgatgccgctggtgcgtgaacagcagcagcagcaacctttggatctgttagtggggggccaacaggatcgccagccccaccatcctgcgcacccgagccaacacaaactgaaaccatctccagatcaggcgttcaaacgccgtaaccatctgcacgagaccttaccacaaccgttg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z