LOCUS       BBa_K1809016             645 bp    DNA     linear       23-Dec-2024
DEFINITION  Const. Promoter-RBS-ChAFP-DT
ACCESSION   BBa_K1809016
VERSION     BBa_K1809016.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..508
                     /label=ChAFP
     CDS             62..508
                     /label=BBa_K1809015
     stem_loop       528..571
                     /label=stem_loop
     misc_feature    517..596
                     /label=BBa_B0010
     terminator      517..596
                     /label=BBa_B0010
     stem_loop       612..631
                     /label=T7 TE
     misc_feature    638..638
                     /label=stop
     polyA_site      632..645
                     /label=polya
     misc_feature    605..645
                     /label=BBa_B0012
     terminator      605..645
                     /label=BBa_B0012
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatgatgctg acggtctccc tgctggtgtg cgctatcgtt gccttgacac gcgctgcgga
      121 cgaatgtccg accgactgga aaatgttcaa cggccgttgc tttctgttta acccgctgca
      181 actgcattgg gcagacgctc aggagtcctg tatgaaagaa ggtgctaacc tggccagtat
      241 tcatagcctt gaggagtcta cttttgtgaa agagctcact tccgccgact tgattccgag
      301 ctggattggc ggcaccgatt gtcaggtgag tacgcgctgg ttttggatgg atagtacaag
      361 tatggattac gcagattggt gcgcagctca gccggataca acactgaccg aatgttgcat
      421 tcaaatgaat gtcggtatcg gcaaatgctg gaacgatact ccgtgcaccc atctgcacag
      481 cagtatttgt gctaagcccc tgaaatgata ctagagccag gcatcaaata aaacgaaagg
      541 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      601 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//