LOCUS       BBa_K1809017             716 bp    DNA     linear       22-Dec-2024
DEFINITION  Const. Promoter-RBS-BclA-ChAFP-DT
ACCESSION   BBa_K1809017
VERSION     BBa_K1809017.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    64..126
                     /label=BBa_K1423000
     CDS             133..579
                     /label=ChAFP
     CDS             133..579
                     /label=BBa_K1809015
     stem_loop       599..642
                     /label=stem_loop
     misc_feature    588..667
                     /label=BBa_B0010
     terminator      588..667
                     /label=BBa_B0010
     stem_loop       683..702
                     /label=T7 TE
     misc_feature    709..709
                     /label=stop
     polyA_site      703..716
                     /label=polya
     misc_feature    676..716
                     /label=BBa_B0012
     terminator      676..716
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gaggcgttcg atcctaatct ggtaggaccc acccttccgc cgatcccgcc attcacactg
      121 ccaacatact agatgatgct gacggtctcc ctgctggtgt gcgctatcgt tgccttgaca
      181 cgcgctgcgg acgaatgtcc gaccgactgg aaaatgttca acggccgttg ctttctgttt
      241 aacccgctgc aactgcattg ggcagacgct caggagtcct gtatgaaaga aggtgctaac
      301 ctggccagta ttcatagcct tgaggagtct acttttgtga aagagctcac ttccgccgac
      361 ttgattccga gctggattgg cggcaccgat tgtcaggtga gtacgcgctg gttttggatg
      421 gatagtacaa gtatggatta cgcagattgg tgcgcagctc agccggatac aacactgacc
      481 gaatgttgca ttcaaatgaa tgtcggtatc ggcaaatgct ggaacgatac tccgtgcacc
      541 catctgcaca gcagtatttg tgctaagccc ctgaaatgat actagagcca ggcatcaaat
      601 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      661 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//