LOCUS       BBa_K1809032            1241 bp    DNA     linear       13-May-2024
DEFINITION  Const. Promoter-RBS-BclA-MpAFP-DT
ACCESSION   BBa_K1809032
VERSION     BBa_K1809032.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    64..126
                     /label=BBa_K1423000
     CDS             133..1104
                     /label=BBa_K1809031
     stem_loop       1124..1167
                     /label=stem_loop
     misc_feature    1113..1192
                     /label=BBa_B0010
     terminator      1113..1192
                     /label=BBa_B0010
     stem_loop       1208..1227
                     /label=T7 TE
     misc_feature    1234..1234
                     /label=stop
     polyA_site      1228..1241
                     /label=polya
     misc_feature    1201..1241
                     /label=BBa_B0012
     terminator      1201..1241
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gaggcgttcg atcctaatct ggtaggaccc acccttccgc cgatcccgcc attcacactg
      121 ccaacatact agatgaacgt ctctcaatcc aactcttttg ggttctggga tggtaccagt
      181 acccaggcgg agatcactca ttcgttcgat cattacattg gctcggcctt tgacgcgtca
      241 aacaataacg tggctgtgac aggcaacgtt tccgcaacct tgaacgtgct ggcgggggac
      301 gacaaggtaa gcatcgatgg taatgtggaa gatgttttgg tcgctgcaaa cgtggcggtc
      361 ctggacatgg gcactggaaa tgaccagctc tacgtggccg gcgatgtgct ggggaaaatt
      421 gacgcaggta caggcaatga tgagatttat atcaaaggcg atgtgtctgc tgccgtggat
      481 gcgggtaccg gtaatgatga agtttatatt ggcggcaatt tgagcggcga cctggacgcc
      541 ggtacggata atgacaatat ccagattggc ggggatgtca acgcagcact caacgcaggt
      601 accggcaacg ataacttgat catcggccat gacgttagcg ggattgttaa catgggtacg
      661 gataacgata cggttgaagt cggtcggacc attaatgctt cgggaaaagt cctgctggat
      721 accggcgatg attcgctgtt ggtgtccgga gacctgttcg gtgaagtaga cggtgggacc
      781 ggtaacgata ccattattat tgcgggtaag gtgtcgggca acatccaggg tggcacgggc
      841 aatgacatcg ttcgtgtgca atctcaggtt tgggccgaag ctaatatttc ccttggtact
      901 ggtgacgacg tacttattgt agagcacgaa ttacatggga ctgtggccgg taacgaaggg
      961 gacgattcaa tttatctcaa attctatacc aaagagcagt ataacaataa cagcgatctg
     1021 cgtaaccgtg tggccaattt tgaacatatc cgggtcagcg atggcgtggt caaagggagc
     1081 ccggccgact ttgccgacta ctaatactag agccaggcat caaataaaac gaaaggctca
     1141 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1201 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//