BBa_K1810002 1 BBa_K1810002 Melittin Optimized for E. coli with Mcherry 2015-09-12T11:00:00Z 2016-02-03T10:46:07Z While the amino acid sequence for melittin was derived from Apis mellifica, the genomic sequence was altered by hand for optimization in E. coli. The mCherry sequence was obtained from Melittin is a antimicrobial peptide found in bee venom that works to cause cell lysis by forming a pore in the membrane of harmful bacteria. While the 2014 Stony Brook iGEM team was able to successful clone the melittin gene into E. coli, expression was inconsistent. The 2015 Stony Brook iGEM team worked to correct this by optimizing the codons for an E. coli cell. Both the optimized and regular versions of melittin were fused with a mCherry tag in order to quantify differences in expression. With the use of a fluorescence plate reader, a near 100 fold increase in expression for the optimized melittin construct was observed. false false _2235_ 4206 26375 9 false TBA false annotation2458005 1 BBa_B0030 range2458005 1 131 145 annotation2458002 1 Melittin Optimus range2458002 1 155 235 annotation2458004 1 BBa_B1002 range2458004 1 959 992 annotation2458003 1 mCherry Tag range2458003 1 236 958 annotation2458006 1 BBa_I13453 range2458006 1 1 130 BBa_K1810002_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagcattaaagaggagaaatactagatgggtattggtgcggttctgaaagttctgaccaccggtctgccggcgctgatctcttggatcaaacgtaaacgtcagcagggtgtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgtaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaaggcctcgagtaataacgcaaaaaaccccgcttcggcggggttttttcgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z