BBa_K1811333 1 BBa_K1811333 CUP1 metallothionein fused to LamB on outer membrane 2015-08-25T11:00:00Z 2015-08-26T12:52:16Z LamB comes from NCBI ID 948548. CUP1 comes from NCBI ID 856450. LamB is a membrane channel that transports maltose and maltodextrins as well as the receptor for phage lambda. Metallothionein is a protein that sequesters 11 copper atoms and detoxifies radicals produced by copper. This part contains two genes fused together that allows for the expression of metallothionein on the outer surface of E. coli. CUP1, the gene coding for metallothionein from S. cerevisiae, is inserted into amino acid position 153 of the LamB gene sequence. false false _2236_ 20146 20146 9 false The stop codon of CUP1 was removed before being inserted into LamB. In making a G Block for this part to be synthesized, a 20 bp overlap with the plasmid pSB1C3 needed to be added to the ends of the sequence. This needed to be done to allow Gibson Assembly to yield our completed plasmid containing this part in pSB1C3. false Samuel Keating BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1811666 1 BBa_K1811666 CUP1 metallothionein fused to LamB with promoter and RBS 2015-08-25T11:00:00Z 2015-08-26T01:03:50Z LamB comes from NCBI ID 948548. CUP1 comes from NCBI ID 856450. LamB is a membrane channel that transports maltose and maltodextrins as well as the receptor for phage lambda. Metallothionein is a protein that sequesters 11 copper atoms and detoxifies radicals produced by copper. This part is a composite of the CUP1 LamB fusion (BBa_K1811333), a strong constituitive promoter (BBa_J23100), and an RBS (BBa_B0034). false false _2236_ 20146 20146 9 false The stop codon of CUP1 was removed before being inserted into LamB. In making a G Block for this part to be synthesized, a 20 bp overlap with the plasmid pSB1C3 needed to be added to the ends of the sequence. This needed to be done to allow Gibson Assembly to yield our completed plasmid containing this part in pSB1C3. false Samuel Keating component2438304 1 BBa_J23100 component2438307 1 BBa_K1811333 component2438306 1 BBa_B0034 annotation2438304 1 BBa_J23100 range2438304 1 1 35 annotation2438307 1 BBa_K1811333 range2438307 1 62 1585 annotation2438306 1 BBa_B0034 range2438306 1 44 55 BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0034_sequence 1 aaagaggagaaa BBa_K1811666_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgatgattactctgcgcaaacttcctctggcggttgccgtcgcagcgggcgtaatgtctgctcaggcaatggctgttgatttccacggctatgcacgttccggtattggttggacaggtagcggcggtgaacaacagtgtttccagactaccggtgctcaaagtaaataccgtcttggcaacgaatgtgaaacttatgctgaattaaaattgggtcaggaagtgtggaaagagggcgataagagcttctatttcgacactaacgtggcctattccgtcgcacaacagaatgactgggaagctaccgatccggccttccgtgaagcaaacgtgcagggtaaaaacctgatcgaatggctgccaggctccaccatctgggcaggtaagcgcttctaccaacgtcatgacgttcatatgatcgacttctactactgggatatttctggtcctggtgccatgtttagcgagctgatcaacttccagaatgaagggcacgagtgtcaatgccagtgcggcagttgcaagaacaacgaacagtgtcagaaatcctgctcctgtccgaccggctgtaacagtgacgataaatgcccatgtgggaacaaatccgaagagacgaaaaaaagttgctgctcaggcaaaggtctggaaaacatcgatgttggcttcggtaaactctctctggcagcaacccgctcctctgaagctggtggttcttcctctttcgccagcaacaatatttatgactataccaacgaaaccgcgaacgacgttttcgatgtgcgtttagcgcagatggaaatcaacccgggcggcacattagaactgggtgtcgactacggtcgtgccaacttgcgtgataactatcgtctggttgatggcgcatcgaaagacggctggttattcactgctgaacatactcagagtgtcctgaagggctttaacaagtttgttgttcagtacgctactgactcgatgacctcgcagggtaaagggctgtcgcagggttctggcgttgcatttgataacgaaaaatttgcctacaatatcaacaacaacggtcacatgctgcgtatcctcgaccacggtgcgatctccatgggcgacaactgggacatgatgtacgtgggtatgtaccaggatatcaactgggataacgacaacggcaccaagtggtggaccgtcggtattcgcccgatgtacaagtggacgccaatcatgagcaccgtgatggaaatcggctacgacaacgtcgaatcccagcgcaccggcgacaagaacaatcagtacaaaattaccctcgcacaacaatggcaggctggcgacagcatctggtcacgcccggctattcgtgtcttcgcaacctacgccaagtgggatgagaaatggggttacgactacaccggtaacgctgataacaacgcgaacttcggcaaagccgttcctgctgatttcaacggcggcagcttcggtcgtggcgacagcgacgagtggaccttcggtgcccagatggaaatctggtggtaa BBa_K1811333_sequence 1 atgatgattactctgcgcaaacttcctctggcggttgccgtcgcagcgggcgtaatgtctgctcaggcaatggctgttgatttccacggctatgcacgttccggtattggttggacaggtagcggcggtgaacaacagtgtttccagactaccggtgctcaaagtaaataccgtcttggcaacgaatgtgaaacttatgctgaattaaaattgggtcaggaagtgtggaaagagggcgataagagcttctatttcgacactaacgtggcctattccgtcgcacaacagaatgactgggaagctaccgatccggccttccgtgaagcaaacgtgcagggtaaaaacctgatcgaatggctgccaggctccaccatctgggcaggtaagcgcttctaccaacgtcatgacgttcatatgatcgacttctactactgggatatttctggtcctggtgccatgtttagcgagctgatcaacttccagaatgaagggcacgagtgtcaatgccagtgcggcagttgcaagaacaacgaacagtgtcagaaatcctgctcctgtccgaccggctgtaacagtgacgataaatgcccatgtgggaacaaatccgaagagacgaaaaaaagttgctgctcaggcaaaggtctggaaaacatcgatgttggcttcggtaaactctctctggcagcaacccgctcctctgaagctggtggttcttcctctttcgccagcaacaatatttatgactataccaacgaaaccgcgaacgacgttttcgatgtgcgtttagcgcagatggaaatcaacccgggcggcacattagaactgggtgtcgactacggtcgtgccaacttgcgtgataactatcgtctggttgatggcgcatcgaaagacggctggttattcactgctgaacatactcagagtgtcctgaagggctttaacaagtttgttgttcagtacgctactgactcgatgacctcgcagggtaaagggctgtcgcagggttctggcgttgcatttgataacgaaaaatttgcctacaatatcaacaacaacggtcacatgctgcgtatcctcgaccacggtgcgatctccatgggcgacaactgggacatgatgtacgtgggtatgtaccaggatatcaactgggataacgacaacggcaccaagtggtggaccgtcggtattcgcccgatgtacaagtggacgccaatcatgagcaccgtgatggaaatcggctacgacaacgtcgaatcccagcgcaccggcgacaagaacaatcagtacaaaattaccctcgcacaacaatggcaggctggcgacagcatctggtcacgcccggctattcgtgtcttcgcaacctacgccaagtgggatgagaaatggggttacgactacaccggtaacgctgataacaacgcgaacttcggcaaagccgttcctgctgatttcaacggcggcagcttcggtcgtggcgacagcgacgagtggaccttcggtgcccagatggaaatctggtggtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z