BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1811222 1 BBa_K1811222 codon optimized CUP1 2015-08-25T11:00:00Z 2015-08-26T08:34:02Z The CUP1 gene comes from NCBI ID 856450 and is native to S. cerevisiae. The gene sequence was then codon optimized for E. coli tRNA abundance. Metallothionein is a heavy metal binding protein native to S. cerevisiae that can bind up to 11 copper atoms. This part contains the gene sequence for CUP1 that has been codon optimized for E. coli tRNA abundance. false false _2236_ 20146 20146 9 false In making a G Block for this gene to be synthesized, a 20 bp overlap with the plasmid pSB1C3 needed to be added to the ends of the sequence. false Samuel Keating BBa_K1811888 1 BBa_K1811888 codon optimized CUP1 copper resistance gene with RBS and promoter 2015-08-25T11:00:00Z 2015-08-26T08:41:22Z The CUP1 gene comes from NCBI ID 856450 and is native to S. cerevisiae. The gene sequence was then codon optimized for E. coli tRNA abundance. Metallothionein is a copper binding protein native to S. cerevisiae that gives cells resistance to copper toxicity by binding 11 copper atoms. It prevents copper from reacting to form toxic molecules when bound and detoxifies free radicals. The part contains the metallothionein gene CUP1 (BBa_K1811222), a strong constituitive promoter (BBa_J23100), and an RBS (BBa_B0034). The CUP1 gene used in this part was codon optimized for E. coli tRNA abundance. false false _2236_ 20146 20146 9 false In making a G Block for this part to be synthesized, a 20 bp overlap with the plasmid pSB1C3 needed to be added to the ends of the sequence. This needed to be done to allow Gibson Assembly to yield our completed plasmid containing this part in pSB1C3. false Samuel Keating component2438109 1 BBa_J23100 component2438112 1 BBa_K1811222 component2438111 1 BBa_B0034 annotation2438109 1 BBa_J23100 range2438109 1 1 35 annotation2438112 1 BBa_K1811222 range2438112 1 62 247 annotation2438111 1 BBa_B0034 range2438111 1 44 55 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_K1811222_sequence 1 atgtttagcgagctgatcaacttccagaatgaagggcacgagtgtcaatgccagtgcggcagttgcaagaacaacgaacagtgtcagaaatcctgctcctgtccgaccggctgtaacagtgacgataaatgcccatgtgggaacaaatccgaagagacgaaaaaaagttgctgctcaggcaaataa BBa_K1811888_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgtttagcgagctgatcaacttccagaatgaagggcacgagtgtcaatgccagtgcggcagttgcaagaacaacgaacagtgtcagaaatcctgctcctgtccgaccggctgtaacagtgacgataaatgcccatgtgggaacaaatccgaagagacgaaaaaaagttgctgctcaggcaaataa BBa_B0034_sequence 1 aaagaggagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z