LOCUS       BBa_K1813001            1588 bp    DNA     linear       21-May-2024
DEFINITION  <i>cch2</i> Expression Cassette
ACCESSION   BBa_K1813001
VERSION     BBa_K1813001.1  
FEATURES             Location/Qualifiers
     promoter        1..40
                     /label=Ptac
     promoter        7..12
                     /label=-35
     promoter        29..33
                     /label=-10
     misc_feature    36..61
                     /label=lacO1 site
     promoter        1..61
                     /label=BBa_K864400
     misc_feature    74..77
                     /label=conserved
     RBS             70..81
                     /label=BBa_B0034
     CDS             88..1485
                     /label=BBa_K1813000
     stem_loop       1501..1520
                     /label=T7 TE
     misc_feature    1527..1527
                     /label=stop
     polyA_site      1521..1534
                     /label=polya
     misc_feature    1494..1534
                     /label=BBa_B0012
     terminator      1494..1534
                     /label=BBa_B0012
     stem_loop       1555..1577
                     /label=stem_loop
     misc_feature    1543..1588
                     /label=BBa_B0011
     terminator      1543..1588
                     /label=BBa_B0011
     terminator      1494..1588
                     /label=BBa_B0014
     terminator      1494..1588
                     /label=BBa_K823017
ORIGIN
        1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat
       61 ttactagaga aagaggagaa atactagatg tcattgattg caattaagaa cgctgactgg
      121 atcttaacca tggatggcgc ccgtcgcgta atcgcagacg gctctatttt aatcgataaa
      181 gaccgtattc gcgccatcgg taaaagtgct gtgatcgaga ttcctccaga gacaaccaaa
      241 gtgattgacg gtcgcggcaa agtggttttg ccaggcttaa ttgacagtca tacccaccac
      301 tcattacact taggccgtgg cttggctgac gaatgtgaca ttcagacatt cttatatcgt
      361 cgcttgtacc caatcgaagc cagtttaaat gacgaggatg cctatatctc agcattatta
      421 tgccagttag aaatgattaa aagtggtacc acctctatca tcgatgctgg caactacttt
      481 ccagaggcta ccttacgtgc atttggcaca acaggtatgc gtggcgttgt tgcacgttca
      541 acatttgaca ttcctacttc atctttgggt tctttacctg ctcaggtgtt tgcagaggag
      601 acaaatgtag ccttaaaacg tgccgaagaa tttgtagagc gtaactctgg cgcatgcgac
      661 ggccgtgtgc aggcatggtt acagttgcgc gttttaccta attgtagtga tgagttgtgc
      721 cgtggtttga aatctattgc tgaccgtttg ggcgttcgct atgaagcaca cgccgccttt
      781 accaaagaag tgtatgaagc tagtaaatta cagttcggca agagtgaagt gcgtcgtttg
      841 gatgatttag gtatcttagg cgaaggttta ttattagctc atatgggttg gttaacccca
      901 cgcgatattt tgttattgat tagttcaaaa actaatgttg tattatgccc aacctctagt
      961 gtacaccagg ctatgggttc tatcgccttc ggccacgtgc cagaattatt agaaatgggt
     1021 gtgaacgttg cattaggtac tgatggtggt cctcacggca caaatgactt ggtacgccag
     1081 attttcgtgg ccgcaggcgg ctataaagaa gttcgcttgg atgctacaat catgcctcca
     1141 gaaacagttt tagaaatggc aaccgttaat ggtgcacgcg ccatgggtat gtctgaccag
     1201 gtaggctcaa tcgagccagg taaaaaagcc gacattacaa tcttcgactc tcgccgccct
     1261 gaatggcgcc cattacataa tcctgttgca aatttagtat attgcgcaaa cggtaattca
     1321 gcagataccg tgattgtaga cggccgcatt ttaatggaaa accgtaaaat cttaaccttt
     1381 aacgaggatg acgtgattac cgaagcacag cgtcgctcag ttgaaatcgg tgctcgtgct
     1441 ggcttgttgg aatacggtcg cccacgctgg ccagttcatt aataatacta gagtcacact
     1501 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagagaata taaaaagcca
     1561 gattattaat ccggcttttt tattattt
//